Development of 15 polymorphic microsatellite markers for Ficus virens (Moraceae)

Abstract

Premise of the study:Ficus virens (Moraceae) is distributed widely in South and Southeast Asia, Melanesia, and northern Australia, and it is also cultivated outside its original northern range limit in southwestern China. Therefore, the species is well suited to explore the mechanism of range limits of Ficus species. However, little is known about its genetic background.Methods and Results:Fifteen polymorphic microsatellite markers were developed using the biotin-streptavidin capture method. Polymorphism was tested in 85 F. virens individuals sampled from three populations. The number of alleles ranged from three to 17. The observed and expected heterozygosity of each population varied from 0.0667 to 0.9286 and 0.0650 to 0.8890, respectively. Cross-species amplification was also carried out in eight other Ficus species.Conclusions:These 15 markers will be valuable for studying the genetic variation and population structure of F. virens and related Ficus species.

Dwarfism of Ficus microcarpa in the Ryukyu islands, Okinawa, Japan

“…Genomic DNA was isolated from samples using the cetyl trimethyl ammonium bromide (CTAB) extraction procedure (Stewart and Via 1993). Polymerase chain reaction (PCR) amplification was performed using a TaKaRa PCR Thermal Cycler Dice Gradient (Takara Bio Inc., Otsu, Shiga, Japan) with 11 nuclear microsatellite markers: LMFC15, 23, 32, 36 (Giraldo et al 2005), Car8, 9, 11 andMicr3 (Garcia et al 2012), V183, 188 (Fu et al 2017) andFT14 (Zhang et al 2016). Two-step multiplex PCR was performed using universal primers for fluorescent labeling of PCR fragments (Blacket et al 2012) together with PIG-tailed reverse primers to promote adenylation (Brownstein et al 1996).…”