Development of 21 microsatellite primers for Camellia pingguoensis (Theaceae) using 454 sequencing

Lu, Yong-Bin; Liufu, Yongqing; Peng, Guo-Qing; Ye, Quanqing; Tang, Shaoqing

doi:10.1007/s12686-014-0221-9

Cited by 2 publications

(1 citation statement)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Microsatellite markers were selected from previously developed microsatellites in Camellia pingguoensis D. Fang ( Lu et al., 2014 ) and Camellia flavida H. T. Chang ( Liufu et al., 2014 ). PCR amplification was carried out in 20 μL reactions containing 2 μL 10 × PCR buffer (Mg 2+ plus), 0.4 μL dNTP mix (10 mM), 0.2 μL each primer (50 μM), 0.2 μL rTaq DNA Polymerase (all reagents from TaKaRa, China), 1.0 μL DNA (20 ng) and 16 μL double-distilled water.…”

Section: Methodsmentioning

confidence: 99%

Genetic diversity and population structure of Camellia huana (Theaceae), a limestone species with narrow geographic range, based on chloroplast DNA sequence and microsatellite markers

Shang-li

Pei

et al. 2020

Plant Diversity

Self Cite

View full text Add to dashboard Cite

Camellia huana is an endangered species with a narrow distribution in limestone hills of northern Guangxi and southern Guizhou provinces, China. We used one chloroplast DNA (cpDNA) fragment and 12 pairs of microsatellite (simple sequence repeat; SSR) markers to assess the genetic diversity and structure of 12 C. huana populations. A total of 99 alleles were detected for 12 polymorphic loci, and eight haplotypes and nine polymorphic sites were detected within 5200 bp of cpDNA. C. huana populations showed a low level of genetic diversity ( n = 8, Hd = 0.759, Pi = 0.00042 for cpDNA, N A = 3.931, H E = 0.466 for SSRs), but high genetic differentiation between populations ( F ST = 0.2159 for SSRs, F ST = 0.9318 for cpDNA). This can be attributed to the narrow distribution and limestone habitat of C. huana . STRUCTURE analysis divided natural C. huana populations into two groups, consistent with their geographical distribution. Thus, we suggest that five natural C. huana populations should be split into two units to be managed effectively.

show abstract

Section: Methodsmentioning

confidence: 99%

Genetic diversity and population structure of Camellia huana (Theaceae), a limestone species with narrow geographic range, based on chloroplast DNA sequence and microsatellite markers

Shang-li

Pei

et al. 2020

Plant Diversity

Self Cite

View full text Add to dashboard Cite

show abstract

Development of EST-SSR markers for an endangered plant species, Camellia fascicularis (Theaceae)

Liu

Jing

et al. 2022

Plant Genet. Resour.

View full text Add to dashboard Cite

The plant Camellia fascicularis, belonging to family Theaceae, has high ornamental and medicinal value, and rare gene resources for genetic improvement of Camellia crops, but is currently threatened with extinction because of the unique and extremely small wild populations. Molecular markers have clarified the wild plant species’ genetic diversity structure, new gene resources and relationship with crops. This will be beneficial for conservation of these valuable crop-related wild species and crop improvement. In this study, we identified 95,979 microsatellite loci from 155,011 transcriptome unigenes, and developed 14 polymorphic expressed sequence tag-derived simple sequence repeat (EST-SSR) microsatellite markers for C. fascicularis. The number of alleles (Na) per locus was 2–8 with a mean of 4.86. The genetic diversity of 40 individuals from four natural populations of C. fascicularis was analysed using these polymorphic markers. The number of alleles (Na) for EST-SSR ranged from 2 to 5, with the expected heterozygosities (He) and observed heterozygosities (Ho) in all loci ranging from 0.183 to 0.683, and from 0.201 to 0.700, respectively, implying a rich genetic variation present in wild C. fascicularis populations. Moreover, the phylogenetic analysis among four populations, using the 14 EST-SSR markers developed in this study, grouped 40 individuals into three groups, which coincide with their geographic distribution. These results showed that 14 EST-SSR markers are available for the analysis of genetic variation in C. fascicularis populations and genetic improvement of new Camellias cultivars by interspecific hybridization, and are beneficial for conservation of the endangered species.

show abstract

Development of 21 microsatellite primers for Camellia pingguoensis (Theaceae) using 454 sequencing

Cited by 2 publications

References 1 publication

Genetic diversity and population structure of Camellia huana (Theaceae), a limestone species with narrow geographic range, based on chloroplast DNA sequence and microsatellite markers

Genetic diversity and population structure of Camellia huana (Theaceae), a limestone species with narrow geographic range, based on chloroplast DNA sequence and microsatellite markers

Development of EST-SSR markers for an endangered plant species, Camellia fascicularis (Theaceae)

Contact Info

Product

Resources

About