Development of 26 microsatellite markers in Bupleurum latissimum (Apiaceae), an endangered plant endemic to Ulleung Island, Korea

Abstract

Premise of the StudyTo enhance our understanding of evolutionary consequences and to establish a suitable conservation strategy, we isolated microsatellite markers for the endangered Bupleurum latissimum (Apiaceae), which is endemic to the oceanic Ulleung Island. We also attempted cross‐amplification in B. euphorbioides and B. longeradiatum to investigate its continental progenitors.Methods and ResultsUsing high‐throughput sequencing data, we developed 26 polymorphic microsatellite loci in three multiplexes, of which 13 loci were polymorphic in the two related species. For B. latissimum, alleles numbered two to four and the observed and expected heterozygosity ranged from 0.000 to 0.500 and 0.061 to 0.529, respectively.ConclusionsThese developed markers will be useful for understanding evolutionary patterns of B. latissimum in an oceanic island system and for establishing suitable conservation strategies at the genetic level.

Rear‐edge, low‐diversity, and haplotypic uniformity in cold‐adapted Bupleurum euphorbioides interglacial refugia populations

“…For the microsatellite analysis, we used 12 nuclear microsatellite markers, Bul007, Bul008, Bul010, Bul015, Bul016, Bul027, Bul030, Bul032, Bul040, Bul045, Bul049, and Bul050, previously developed for B. euphorbioides and B. longiradiatum (Lee, Yoon, Han, & Kim, 2018). PCR amplification was performed as described in Lee et al.…”

“…PCR amplification was performed as described in Lee et al. (2018). The PCR products were analyzed using an ABI 3730 xl DNA Analyzer, with the GeneScan™ 500 LIZ™ Size Standard (Applied Biosystems).…”