Development of 36 SNP markers in Ophiocephalus argus Cantor based on high-throughput sequencing

Xiao, Mingsong; Hu, Qinsen; Zhao, Yan; Bao, Fangyin; Cui, Feng; Zheng, Rongquan

doi:10.1007/s12686-017-0757-6

Cited by 7 publications

(3 citation statements)

References 5 publications

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“…The argus snakehead ( Ophiocephalus argus Cantor) belongs to the family Channidae, perciformes, and is commonly found in the Yangtze River basin and the lower Yellow River in China, as well as in various river systems in Korea, Japan, and Russia ( Courtenay & Williams, 2004 ). O. argus is a very popular, nutritious, economically-farmed freshwater fish ( Xiao et al, 2017 ). In China, the annual production of O. argus is about 510,000 tons (worth approximately 1.6 billion US dollars) ( Sagada et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

Hematological and cytochemical characteristics of peripheral blood cells in the argus snakehead (Ophiocephalus argus Cantor)

Wang

et al. 2021

PeerJ

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Background The argus snakehead (Ophiocephalus argus Cantor) is a highly nutritious, freshwater, cultured bony fish with a high economic value. The health of the fish is closely related to its blood cells, which are critical for oxygen transport, natural defense, and immunity. We investigated the morphometry, microstructure, and cytochemical characteristics of the peripheral blood cells of O. argus. Our results may provide the basic reference values needed to monitor the health of this fish for large-scale cultivation. Methods The number of blood cells in O. argus were counted on a hemocytometer and their size was measured using a micrometer under light microscope. The morphology and classification of the blood cells were studied using Wright’s staining and the cytochemical characteristics were studied using seven chemical stains including peroxidase (POX), Sudan black B (SBB), periodic acid-Schiff (PAS), acid phosphatase (ACP), alkaline phosphatase (ALP), chloroacetic acid AS-D naphthol esterase (AS-D), and α-naphthol acetate esterase (α-NAE). Results The peripheral blood cells in O. argus can be classified as erythrocytes, leukocytes, and thrombocytes; of which, females had 2.9597 million/mm3, 88,400/mm3, and 43,600/mm3, respectively, and males had 3.0105 million/mm3, 105,500/mm3, and 34,000/mm3, respectively. Leukocytes consisted of neutrophils, monocytes, large lymphocytes, and small lymphocytes. Eosinophils and basophils were not found. Monocytes were the most numerous leukocytes identified, followed by neutrophils and small lymphocytes, while large lymphocytes were the least frequently identified. Cytochemical staining showed that erythrocytes were only positive for PAS staining. Neutrophils were strongly positive for POX, SBB, and ACP, and positive for all the other cytochemical stains. Monocytes were positive for PAS and α-NAE and were weakly positive for ACP and AS-D staining. Large lymphocytes were positive for PAS and were weakly positive for ALP, AS-D, and α-NAE staining. Small lymphocytes were positive for PAS and weakly positive for AS-D and α-NAE staining. Thrombocytes were positive for PAS and were weakly positive for ACP and AS-D, but negative for the remaining cytochemical stains. The morphology of peripheral blood cells in O. argus was generally similar to that of other fish species, while the cytochemical staining patterns showed clear species specificity.

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Section: Introductionmentioning

confidence: 99%

Hematological and cytochemical characteristics of peripheral blood cells in the argus snakehead (Ophiocephalus argus Cantor)

Wang

et al. 2021

PeerJ

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“…In contrast with conventional approaches, RAD-seq approach is considered to be faster and pro table. Furthermore, this method can produce a large number of microsatellite arkers for one time (Peng et al 2018;Xiao et al 2018).…”

Section: Discussionmentioning

confidence: 99%

“…The major advantage of Next generation sequencing (NGS) technique is their ability to produce large amounts of sequence data and it can provide wide genetic information of simple sequence repeat (SSR) or microsatellite loci. Restriction-site associated DNA sequencing (RAD-seq) is a powerful tool to develop microsatellite and single nucleotide polymorphism (SNP) markers, which uses Illumina next-generation sequencing (NGS) platforms to discover informative volume of independent markers in a lot of specimens at the same time (Feng et al 2014;Xiao et al 2018;Khoshkholgh and Nazari, 2020).…”

Section: Introductionmentioning

confidence: 99%

Characterization of novel genotyping-by-sequencing (GBS)-based simple sequence repeats (SSRs) and their application for population genomics of Capoeta aculeata (Valenciennes, 1844)

et al. 2021

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The species Capoeta aculeata (Valenciennes, 1844) is one of the most important freshwater species endemic to Iran. However, the investigation of a population genetic structure of this species is limited by the low number of molecular markers currently described. In this study, we implemented next generation sequencing technology to identify polymorphic microsatellite markers and investigate the population genetic structure of C. aculeata sampled from three geographical sites in Iran. We sequenced 60 individuals from three populations occurring in the Zagros basin. We characterized and developed 36 novel polymorphic microsatellite markers and these loci were examined in 120 individuals from three populations occurring in the Zagros basin. The average number of alleles per locus varied from 1.7 to 16. (average = 7.89). The results showed that, the polymorphism information content (PCI) of these SSR loci varied from 0.254 to 0.888. The observed heterozygosity (Ho) per locus ranged from 0.170 to 0.881, while the expected heterozygosity (He) per locus was from 0.170 to 0.881. Among these SSR loci, 20 loci deviated signi cantly from the Hardy-Weinberg equilibrium after Bonferroni correction (p< 0.05). These microsatellite markers could provide a valuable tool for future population and conservation genetics studies of C. aculeate populations and other closely related species.

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Transcriptome sequencing and de novo analysis of the northern snakehead, Ophiocephalus argus

Xiao

Bao

Zhao

et al. 2019

J Genet

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Development of 36 SNP markers in Ophiocephalus argus Cantor based on high-throughput sequencing

Cited by 7 publications

References 5 publications

Hematological and cytochemical characteristics of peripheral blood cells in the argus snakehead (Ophiocephalus argus Cantor)

Hematological and cytochemical characteristics of peripheral blood cells in the argus snakehead (Ophiocephalus argus Cantor)

Characterization of novel genotyping-by-sequencing (GBS)-based simple sequence repeats (SSRs) and their application for population genomics of Capoeta aculeata (Valenciennes, 1844)

Transcriptome sequencing and de novo analysis of the northern snakehead, Ophiocephalus argus

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