Development of 3D bioprinted GelMA-alginate hydrogels with tunable mechanical properties

Aldana, Ana Agustina; Valente, Filippo; Dilley, Rodney J.; Doyle, Barry

doi:10.1016/j.bprint.2020.e00105

Cited by 76 publications

(47 citation statements)

References 37 publications

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“…The blended GelMA-chitosan hydrogel improved mechanical properties compared to the gelatin-chitosan hydrogel, and its biocompatibility was demonstrated by analysis of the spread area of bone mesenchymal stem cells on the blended hydrogel surface. Additionally, the blended GelMA-alginate hydrogels were fabricated by an extrusion-based bioprinting method [19]. GelMA was mixed with alginate hydrogel to optimize printing conditions.…”

Section: Introductionmentioning

confidence: 99%

Conductive GelMA–Collagen–AgNW Blended Hydrogel for Smart Actuator

Lim

Cho

et al. 2021

Polymers

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Blended hydrogels play an important role in enhancing the properties (e.g., mechanical properties and conductivity) of hydrogels. In this study, we generated a conductive blended hydrogel, which was achieved by mixing gelatin methacrylate (GelMA) with collagen, and silver nanowire (AgNW). The ratio of GelMA, collagen and AgNW was optimized and was subsequently gelated by ultraviolet light (UV) and heat. The scanning electron microscope (SEM) image of the conductive blended hydrogels showed that collagen and AgNW were present in the GelMA hydrogel. Additionally, rheological analysis indicated that the mechanical properties of the conductive GelMA–collagen–AgNW blended hydrogels improved. Biocompatibility analysis confirmed that the human umbilical vein endothelial cells (HUVECs) encapsulated within the three-dimensional (3D), conductive blended hydrogels were highly viable. Furthermore, we confirmed that the molecule in the conductive blended hydrogel was released by electrical stimuli-mediated structural deformation. Therefore, this conductive GelMA–collagen–AgNW blended hydrogel could be potentially used as a smart actuator for drug delivery applications.

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Section: Introductionmentioning

confidence: 99%

Conductive GelMA–Collagen–AgNW Blended Hydrogel for Smart Actuator

Lim

Cho

et al. 2021

Polymers

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“…To improve these properties, a variety of covalent crosslinking methods have been used. For example, Aldana et al developed an alginate-based bioink with tunable mechanical properties using blends of alginate and gelatin methacrylamide (GelMA), obtaining a photopolymerizable biomaterial with different printability, accuracy, and mechanical and biological properties, depending on the ratio of alginate:GelMA [19].…”

Section: Alginate Based Bioinksmentioning

confidence: 99%

“…For this reason, the use of derivatives or blends with different polymers are usually required to obtain appropriate mechanical properties for their use. An example is the modification of gelatin with methacrylamide to obtain a photopolymerizable biomaterial that can be used for 3D bioprinting and microfluidics [19][20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Emerging Biofabrication Techniques: A Review on Natural Polymers for Biomedical Applications

2021

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Natural polymers have been widely used for biomedical applications in recent decades. They offer the advantages of resembling the extracellular matrix of native tissues and retaining biochemical cues and properties necessary to enhance their biocompatibility, so they usually improve the cellular attachment and behavior and avoid immunological reactions. Moreover, they offer a rapid degradability through natural enzymatic or chemical processes. However, natural polymers present poor mechanical strength, which frequently makes the manipulation processes difficult. Recent advances in biofabrication, 3D printing, microfluidics, and cell-electrospinning allow the manufacturing of complex natural polymer matrixes with biophysical and structural properties similar to those of the extracellular matrix. In addition, these techniques offer the possibility of incorporating different cell lines into the fabrication process, a revolutionary strategy broadly explored in recent years to produce cell-laden scaffolds that can better mimic the properties of functional tissues. In this review, the use of 3D printing, microfluidics, and electrospinning approaches has been extensively investigated for the biofabrication of naturally derived polymer scaffolds with encapsulated cells intended for biomedical applications (e.g., cell therapies, bone and dental grafts, cardiovascular or musculoskeletal tissue regeneration, and wound healing).

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“…Blended polymers have been used to complement the characteristics of each polymer material to improve printability and mechanical, physicochemical, and biological properties of the bioink [55]. In particular, photo-cross-linkable polymers (e.g., PEGDA (poly(ethylene glycol) diacrylate) and GELMA (gelatin methacrylate)) are broadly used as solidifiers, and contribute to the solidification of blended bioinks using UV light [56,57]. Moreover, several additives (e.g., graphene oxides, hydroxyapatite, nano-cellulose) are added as supplements into polymer bioinks to improve their specific functionality (e.g., fidelity, differentiation) [58][59][60].…”

Section: Technologies For Bioinksmentioning

confidence: 99%

3D Bioprinting of In Vitro Models Using Hydrogel-Based Bioinks

Choi

Park

Ha³

et al. 2021

Polymers

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Coronavirus disease 2019 (COVID-19), which has recently emerged as a global pandemic, has caused a serious economic crisis due to the social disconnection and physical distancing in human society. To rapidly respond to the emergence of new diseases, a reliable in vitro model needs to be established expeditiously for the identification of appropriate therapeutic agents. Such models can be of great help in validating the pathological behavior of pathogens and therapeutic agents. Recently, in vitro models representing human organs and tissues and biological functions have been developed based on high-precision 3D bioprinting. In this paper, we delineate an in-depth assessment of the recently developed 3D bioprinting technology and bioinks. In particular, we discuss the latest achievements and future aspects of the use of 3D bioprinting for in vitro modeling.

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Development of 3D bioprinted GelMA-alginate hydrogels with tunable mechanical properties

Cited by 76 publications

References 37 publications

Conductive GelMA–Collagen–AgNW Blended Hydrogel for Smart Actuator

Conductive GelMA–Collagen–AgNW Blended Hydrogel for Smart Actuator

Emerging Biofabrication Techniques: A Review on Natural Polymers for Biomedical Applications

3D Bioprinting of In Vitro Models Using Hydrogel-Based Bioinks

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