Development of 3D wet-spun polymeric scaffolds loaded with antimicrobial agents for bone engineering

Abstract

Three-dimensional wet-spun microfibrous meshes of a star poly(ε-caprolactone) were developed as potential scaffolds endowed with antimicrobial activity. The in vitro release kinetics of the meshes, under physiological conditions, was initially fast and then a sustained release for more than one month was observed. Cell cultures of a murine pre-osteoblast cell line showed good cell viability and adhesion on the wet-spun star poly(ε-caprolactone) fiber scaffolds. These promising results indicate a potential application of the developed meshes as engineered bone scaffolds with antimicrobial activit

Microscale Cell Encapsulation Materials and Fabrication Techniques for Type 1 Diabetes

“…They are easy to handle and can be readily used to build a porous 3D scaffold [61]. In contrast to wet spinning, in which the crosslinking agent is added to a bath [62], microfluidic spinning forms microfibers via the coaxial flow of polymer solution and crosslinking agent in a microchannel [63]. Microfluidic spinning allows microfibers to be continuously fabricated and their diameter to be finely tuned from several to a few hundred microns by simply controlling the flow rate.…”

Microtechnologies in the Fabrication of Fibers for Tissue Engineering

“…28 They coated the fibers with poly-L-lysine (PLL) and then seeded with Schwann cells for neural tissue engineering. Cell attachment and proliferation was much higher on the fiber 16 The fabricated fibers were porous and had diameters in the range of 100-300 mm. The fibers were randomly deposited to form a 3D structure, which was then seeded with murine pre-osteoblasts for bone tissue engineering.…”

“…[18][19][20] To achieve chemically and mechanically stable fibers, the pre-polymer and the final polymer should be insoluble in the coagulation solution. 16 Fibers fabricated by this method can be deposited randomly or in a predefined pattern in the coagulation bath to form a porous scaffold or can be collected on a motorized spool and assembled in a consequent process. Wetspinning has been widely used for fabrication of fibers from various biocompatible materials including alginate, collagen-alginate composite, 9 collagen, 13 chitosan, 21 poly e-caprolactone (PCL), 16 starch-PCL composite, 22 chitosan-tripolyphosphate composite, 23 and calcium phosphate cement-alginate composite.…”

“…16 Fibers fabricated by this method can be deposited randomly or in a predefined pattern in the coagulation bath to form a porous scaffold or can be collected on a motorized spool and assembled in a consequent process. Wetspinning has been widely used for fabrication of fibers from various biocompatible materials including alginate, collagen-alginate composite, 9 collagen, 13 chitosan, 21 poly e-caprolactone (PCL), 16 starch-PCL composite, 22 chitosan-tripolyphosphate composite, 23 and calcium phosphate cement-alginate composite. 9 Fiber diameter can be adjusted by controlling the polymer composition and viscosity, the injection flow rate, and the diameter of the spinneret.…”

“…The system is usually simple and includes a reservoir for the pre-polymer, a spinneret, and a coagulation bath (Figure 1.3a). Prepolymers can be injected manually, 13,14 gravitationally, 15 using a syringe pump, 16,17 or using pressurized air. [18][19][20] To achieve chemically and mechanically stable fibers, the pre-polymer and the final polymer should be insoluble in the coagulation solution.…”