Ocellated ice sh (Chionodraco rastrospinosus, C. rastrospinosu), is played an essential role in the Antarctic food web, which is mainly distributed in the deep waters of the Southern Ocean. The abundance of this species has been drastically reduced due to various impacts, including over shing and climate change. Therefore, in order to sustainably exploit and protect this species, it is urgent to understand the state of its population resources. However, the population genetics and effective molecular markers of this species have not been reported. In this study, 105 novel single nucleotide polymorphism (SNP) markers were developed using double digest restriction site-associated DNA sequencing (ddRAD-Seq). The observed heterozygosity (H o ) and expected heterozygosity (H e ) ranged from 0 to 1 and 0.2188 to 0.5, respectively. The polymorphism information content (PIC) value ranged from 0.194824 to 0.375. After Bonferroni correction, none of the loci deviated signi cantly from Hardy-Weinberg equilibrium (p 0.05). The development of these SNP markers will be useful for the genetic analysis and scienti c management of C. rastrospinosus.
FulltextThe ice sh Chionodraco rastrospinosus (Channichthyidae) is native to the Southern Ocean. It is characterized by having translucent blood, mainly as a result of the lack of oxygen-binding hemoglobin (Kock. 2005). This species is endemic to the Antarctic Peninsula, South Orkneys, South Shetlands, and Elephant Island (Kock et al. 1993). Demersal sh stocks were depleted in these areas by industrial shing from the late 1970s to the early 1980s, which also severely damaged the habitat of C. rastrospinosus (Marschoff et al. 2012). In addition, the morphological characteristics of C. rastrospinosus are similar to other Chionodraco species, which makes it di cult to complete species identi cation based on appearance alone (Wei et al. 2022). Previous studies of C. rastrospinosus focused on analyzing trace elements, its life history, and otolith composition (
