Development of a 2D laser ablation inductively coupled plasma mass spectrometry mapping procedure for mercury in maize (Zea mays L.) root cross-sections

Debeljak, Marta; Elteren, Johannes T. van; Vogel‐Mikuš, Katarina

doi:10.1016/j.aca.2013.05.053

Cited by 40 publications

(25 citation statements)

References 37 publications

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“…Kutscher et al 153 developed a method for the determination of ovalbumin labeled with p-hydroxy-mercuribenzoic acid (pHMB). Polyacrylamide GE was used for the protein separation followed by Hg quantication using ICP-MS. Two different quantication strategies were applied: external calibration using standards of the derivatized protein aer 13 C normalization and, as a proof of concept, SSID using pHMB spiked with 199 Hg. Due to the inhomogeneous distribution of the protein within the gel bands, it could be demonstrated that the ID approach was superior in terms of precision and accuracy.…”

Section: Isotope Dilutionmentioning

confidence: 99%

Review of the applications of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to the analysis of biological samples

Pozebon

Scheffler

Dressler

et al. 2014

J. Anal. At. Spectrom.

164

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Section: Isotope Dilutionmentioning

confidence: 99%

Review of the applications of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to the analysis of biological samples

Pozebon

Scheffler

Dressler

et al. 2014

J. Anal. At. Spectrom.

164

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“…2a). This partitioning of mercury was observed also in other plants, such as Rumexinduratus, Marrubium vulgare, Medicagosativa and maize (Carrasco-Gil et al, 2013;Debeljak et al, 2013;Moreno-Jiménez et al, 2006). The highest Hg concentrations were seen in the 50 Hg treatment.…”

Section: Hg and Se Concentrations In Plant Tissuesmentioning

confidence: 60%

“…Hg and Se concentrations in plants(4 per treatment)and Hg concentrations in animals(10 per treatment) were determined by ICP-MS (Agilent 7500ce, Palo Alto, CA USA) after microwave assisted digestion (MarsXPress, CEM, 15 min ramp to 180C, 30 min hold at 180C, 1000 W) in concentrated HNO 3 and stabilization of digests by HCl (Debeljak et al, 2013). The results were validated by measuring standard reference materials (BCR/CRM-061 Aquatic plant, NIST; ERM Tuna fish, Sigma Aldrich).…”

Section: Hg and Se Analysis With Icp-msmentioning

confidence: 99%

The Effect of Selenium on Mercury Transport Along the Food Chain

Zidar¹,

Kržišnik²,

Debeljak³

et al. 2016

AGR

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More than 500 years of mercury (Hg) production in Idrija (Slovenia) resulted in a considerable pollution of Idrija region with Hg. Although the mine is closed for more than 20 years, the total soil concentration of Hg may still reach up to several hundred mgkg -1 dry weightin local gardens and more that thousand inother urban regions. Hg in soil undergoesdifferent chemical transformations and in some formsit may enterplants and higher trophic levelsin food chains, also with biomagnification pattern.The local population is, besides air and dust, thus exposed to mercury also via consumption of locally produced food.Several studies showed that the increased level of selenium in soil may reduce the uptake of mercury in plants but very few include other trophic levels in a food chain as well.In our pilot study we followed an impact of Seon Hg transport from soil to plants (Lactucasativa) and further to soil dwelling animals (Porcellioscaber). Lettuce was planted in a contaminated soil from Idrija and in soil with added HgCl 2 . The leaves of half of the plants weresprayed with Sesolution (5µg L -1 )threeand five weeks after planting.After six weeks plants were analyzed for Hg and Se and offered as food to terrestrial isopods for two weeks. Our preliminary results revealed that foliar treatment of plants with Se may affect Hg accumulation in plants and therefore further transport of Hg across the food chain.

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“…Although the soil scattered the light and modified the circularity of the laser beam, the distortions were minor. The dissections achieved were instantaneous and precise as is typically observed when lasers are used, for example, in mass spectrometry studies (Debeljak et al 2013 ). Coupling large scale dissection to live imaging was more challenging because of cutting debris reducing image contrast.…”

Section: Discussionmentioning

confidence: 84%

In situ laser manipulation of root tissues in transparent soil

Dupuy

MacDonald

2021

Plant Soil

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Aims Laser micromanipulation such as dissection or optical trapping enables remote physical modification of the activity of tissues, cells and organelles. To date, applications of laser manipulation to plant roots grown in soil have been limited. Here, we show laser manipulation can be applied in situ when plant roots are grown in transparent soil. Methods We have developed a Q-switched laser manipulation and imaging instrument to perform controlled dissection of roots and to study light-induced root growth responses. We performed a detailed characterisation of the properties of the cutting beams through the soil, studying dissection and optical ablation. Furthermore, we also studied the use of low light doses to control the root elongation rate of lettuce seedlings (Lactuca sativa) in air, agar, gel and transparent soil. Results We show that whilst soil inhomogeneities affect the thickness and circularity of the beam, those distortions are not inherently limiting. The ability to induce changes in root elongation or complete dissection of microscopic regions of the root is robust to substrate heterogeneity and microscopy set up and is maintained following the limited distortions induced by the transparent soil environment. Conclusions Our findings show that controlled in situ laser dissection of root tissues is possible with a simple and low-cost optical set-up. We also show that, in the absence of dissection, a reduced laser light power density can provide reversible control of root growth, achieving a precise “point and shoot” method for root manipulation.

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Development of a 2D laser ablation inductively coupled plasma mass spectrometry mapping procedure for mercury in maize (Zea mays L.) root cross-sections

Cited by 40 publications

References 37 publications

Review of the applications of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to the analysis of biological samples

Review of the applications of laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) to the analysis of biological samples

The Effect of Selenium on Mercury Transport Along the Food Chain

In situ laser manipulation of root tissues in transparent soil

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