Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics

Hackenhaar, Camila Regina; Rosa, Carolina Flores; Flores, Elí Emanuel Esparza; Santagapita, Patricio R.; Klein, Manuela Poletto; Hertz, Plinho Francisco

doi:10.1016/j.carbpol.2022.119483

Cited by 30 publications

(4 citation statements)

References 35 publications

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“…Noteworthy, it was previously reported that both the free and the GA-whey protein isolate grafted Carr beads iβ-GLs manifested their topmost activity at the same pH range 22 . Analogous situations were also recorded after β-GL entrapment within agar 37 and after its covalent binding to tannic acid stabilized silver nano-particles 40 and to genipin grafted alginate-gelatin 41 .…”

Section: Resultsmentioning

confidence: 73%

Glutaraldehyde-pea protein grafted polysaccharide matrices for functioning as covalent immobilizers

Wahba

2023

Sci Rep

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Three polysaccharide matrices (κ-Carrageenan (Carr), gellan gum, and agar) were grafted via glutaraldehyde (GA) and pea protein (PP). The grafted matrices covalently immobilized β-d-galactosidase (β-GL). Nonetheless, grafted Carr acquired the topmost amount of immobilized β-GL (iβ-GL). Thus, its grafting process was honed via Box-Behnken design and was further characterized via FTIR, EDX, and SEM. The optimal GA-PP-Carr grafting comprised processing Carr beads with 10% PP dispersion of pH 1 and 25% GA solution. The optimal GA-PP-Carr beads acquired 11.44 Ug−1 iβ-GL with 45.49% immobilization efficiency. Both free and GA-PP-Carr iβ-GLs manifested their topmost activity at the selfsame temperature and pH. Nonetheless, the β-GL Km and Vmax values were reduced following immobilization. The GA-PP-Carr iβ-GL manifested good operational stability. Moreover, its storage stability was incremented where 91.74% activity was offered after 35 storage days. The GA-PP-Carr iβ-GL was utilized to degrade lactose in whey permeate with 81.90% lactose degradation efficiency.

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Section: Resultsmentioning

confidence: 73%

Glutaraldehyde-pea protein grafted polysaccharide matrices for functioning as covalent immobilizers

Wahba

2023

Sci Rep

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“…There are literature data on another application of enzymes immobilized in gel, particularly their multiple uses in analyses. For example, β-galactosidase was immobilized on a biocomposite of alginate and gelatin crosslinked with genipin, and it maintained 90% relative activity after 11 reuses in a batch process of lactose hydrolysis [68]. The electrode based on glucose oxidase immobilized onto gelatin by cross-linking with chromium III acetate retained more than 60% of its activity after 20 repeated uses [69].…”

Section: Methods Of Producing Preparations Based On Enzymes Immobiliz...mentioning

confidence: 99%

Enzymes Immobilized into Starch- and Gelatin-Based Hydrogels: Properties and Application in Inhibition Assay

Esimbekova,

Torgashina,

Nemtseva

et al. 2023

Micromachines

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The present work is a review of the research on using hydrogels based on natural biodegradable polymers, starch, and gelatin for enzyme immobilization. This review addresses the main properties of starch and gelatin that make them promising materials in biotechnology for producing enzyme preparations stable during use and storage and insensitive to chemical and physical impacts. The authors summarize their achievements in developing the preparations of enzymes immobilized in starch and gelatin gels and assess their activity, stability, and sensitivity for use as biorecognition elements of enzyme inhibition-based biosensors.

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“…This shift arises from the pine residue, which mitigates repulsion between the negatively charged enzyme groups and the pectin by positioning itself within the hydrogel pores, thus increasing the separation between these groups. Similarly, in another study [71], lactose hydrolysis was conducted utilizing β-galactosidase immobilized within a hydrogel composed of alginate and gelatin crosslinked with genipin, a natural crosslinking agent derived from gardenia fruit. The immobilized enzyme exhibited remarkable longevity, retaining 90% of its initial activity after 11 operational cycles in a batch reactor and maintaining 80% of its initial activity even after 175 days of storage in a freezer.…”

Section: Immobilization Of β-Galactosidase By Entrapmentmentioning

confidence: 99%

Hydrolysis of Lactose: Conventional Techniques and Enzyme Immobilization Strategies on Polymeric Supports

Lucas Vallejo-García,

Cutillo-Foraster,

Arnaiz

et al. 2024

Food Science and Nutrition

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This chapter explores lactose hydrolysis, emphasizing conventional techniques and the noteworthy immobilization of β-galactosidase on polymeric matrices to enhance the process. Lactose, present in milk and dairy, poses challenges for lactose-intolerant individuals, requiring enzymatic hydrolysis for lactose-free product development. The presence of other milk components, such as proteins and minerals, can indirectly influence the efficiency of lactose hydrolysis by potentially interacting with β-galactosidase enzyme or affecting its stability and activity, making it necessary to control factors such as enzyme concentration, temperature, pH, and reaction time to improve lactose hydrolysis rates. The chapter delves into established methodologies, covering enzymatic kinetics, reaction conditions, and substrate concentrations. It also describes the innovative approach of immobilizing β-galactosidase on polymeric supports to enhance enzyme stability, reusability, and overall efficiency in lactose hydrolysis. Discussions include the design of suitable polymeric matrices, providing insights into mechanisms governing catalytic performance. This comprehensive exploration contributes to understanding lactose hydrolysis, offering valuable insights for developing efficient and sustainable enzymatic processes applicable to the food and pharmaceutical industries.

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Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics

Cited by 30 publications

References 35 publications

Glutaraldehyde-pea protein grafted polysaccharide matrices for functioning as covalent immobilizers

Glutaraldehyde-pea protein grafted polysaccharide matrices for functioning as covalent immobilizers

Enzymes Immobilized into Starch- and Gelatin-Based Hydrogels: Properties and Application in Inhibition Assay

Hydrolysis of Lactose: Conventional Techniques and Enzyme Immobilization Strategies on Polymeric Supports

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