Development of a Biodegradable Composite Scaffold for Bone Tissue Engineering: Physicochemical, Topographical, Mechanical, Degradation, and Biological Properties

Preparation of siloxane-containing vaterite/poly (L-lactic acid) hybrid microbeads with silicate and calcium ions-releasing ability

“…PLLA (LACEA µ ; molecular weight, Mw; ³170 kDa) and SiV particles were melt-blended at 200°C to obtain the SiPVH containing 10, 20 and 60 wt % of SiV, hereafter, these hybrids are denoted by SiPVH 10 , SiPVH 20 and SiPVH 60 , respectively. The beads were prepared via an electrospraying method.…”

“…The beads were prepared via an electrospraying method. 12) For the investigation of SiV particle content on the bead morphology and ion-releasing ability, slurries for the electrospraying was prepared by dissolving 1.1 g of SiPVH 10 , 1.3 g of SiPVH 20 and 2.5 g of SiPVH 60 containing 0.1, 0.3 and 1.5 g of SiV particles, respectively into 19.0 g of chloroform. The final PLLA to chloroform of 5% in mass ratio was achieved for the slurries.…”

“…50 mg of the SiPVH 10 beads prepared with the slurry-feeding rate of 0.7 ml/min and the SiPVH 20 and SiPVH 60 beads prepared with the rate of 0.5 ml/min were soaked in 10 ml of Tris buffer solution of pH 7.4 and maintained at 37°C for 168 h. After the soaking, the beads were filtrated and dried at 50°C for 1 d. The weight-average molecular weights (M w ) of PLLA in the beads after soaking were estimated with a gel permeation chromatograph (GPC) (Shimadzu, RID-10A), equipped with two columns (Shodex, KF-604). Si 4+ and Ca 2+ ion concentrations in the solutions were evaluated with an inductively coupled plasma atomic emission spectrometer (ICP-AES) (Shimadzu, ICPS-7000).…”

“…Figures 1(a c) shows the entire shapes of SiPVH beads. The average diameters of the SiPVH 10 , SiPVH 20 and SiPVH 60 beads were measured to be ³110, ³150 and ³130¯m, respectively. Depressed areas can be observed on the surface of the beads, while some of the beads have shrunk and remained spherical.…”

“…Therefore these surface roughness may enable entanglement and remain in regeneration site. Figures 1(d f) shows the SEM images of the fracture face of SiPVH 10 , SiPVH 20 and SiPVH 60 . In the SiPVH 10 and the SiPVH 20 beads, SiV particles are embedded and enclosed by the PLLA matrix.…”