Development of a Biological Dosimeter for Translocation Scoring Based on Two-Color Fluorescence in Situ Hybridization of Chromosome Subsets

Popp, Susanne; Cremer, Thomas

doi:10.1269/jrr.33.supplement_61

Cited by 8 publications

(2 citation statements)

References 24 publications

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“…An RBE of 4.3 ± 0.6 was determined for micronucleus formation in gill cells (7), and RBEs of 3–7 were determined for mutations occurring in the male germ line at specific loci (5, 6). Both used 1.3 MeV average energy fission neutrons, which are near the bottom end of the range studied here.…”

Section: Discussionmentioning

confidence: 99%

Biological Effects of High-Energy Neutrons MeasuredIn VivoUsing a Vertebrate Model

et al. 2009

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Interaction of solar protons and galactic cosmic radiation with the atmosphere and other materials produces high-energy secondary neutrons from below 1 to 1000 MeV and higher. Although secondary neutrons may provide an appreciable component of the radiation dose equivalent received by space and high-altitude air travelers, the biological effects remain poorly defined, particularly in vivo in intact organisms. Here we describe the acute response of Japanese medaka (Oryzias latipes) embryos to a beam of high-energy spallation neutrons that mimics the energy spectrum of secondary neutrons encountered aboard spacecraft and high-altitude aircraft. To determine RBE, embryos were exposed to 0-0.5 Gy of high-energy neutron radiation or 0-15 Gy of reference γ radiation. The radiation response was measured by imaging apoptotic cells in situ in defined volumes of the embryo, an assay that provides a quantifiable, linear dose response. The slope of the dose response in the developing head, relative to reference γ radiation, indicates an RBE of 24.9 (95% CI 13.6-40.7). A higher RBE of 48.1 (95% CI 30.0-66.4) was obtained based on overall survival. A separate analysis of apoptosis in muscle showed an overall nonlinear response, with the greatest effects at doses of less than 0.3 Gy. Results of this experiment indicate that medaka are a useful model for investigating biological damage associated with high-energy neutron exposure.

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Section: Discussionmentioning

confidence: 99%

Biological Effects of High-Energy Neutrons MeasuredIn VivoUsing a Vertebrate Model

et al. 2009

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“…Scoring of chromosome translocations in human lymphocytes could be a useful indicator of cumulative damage [24,26]. Further evaluation of cumulative eects and prospective monitoring of persons with chromosome and micronucleus ®ndings will enable determination of the full signi®cance of exposure to low doses of ionizing radiation emitted by internally incorporated radionuclides and evaluation as to whether they are prognostically associated with an increased risk for adverse health consequences.…”

Section: Discussionmentioning

confidence: 99%

Chromosome analysis of lymphocytes from radiation workers in tritium-applying industry

Joksić

Spasojević-Tišma

1998

International Archives of Occupational and Environmental Health

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Tritium in the chemical form of luminous paint is a more powerful clastogenic agent than tritium gas. Statistical analysis demonstrated that lymphocyte effects are due to the concentration of tritium in urine rather than to the duration of occupational exposure. Since the radiation hazard measured by the MN assay did not differ from that determined by the CA assay, the MN test can be recommended as a rapid assay for screening purposes in cases of occupational exposure to low radiation doses.

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Dose‐ and time‐dependent responses for micronucleus induction by X‐rays and fast neutrons in gill cells of medaka (Oryzias latipes)

Takai

Kagawa

Fujikawa

2004

Environ and Mol Mutagen

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Medaka fish (Oryzias latipes) were exposed to various doses of X-rays or fast neutrons, and the frequency of micronucleated cells (MNCs) was measured in gills sampled at 12- or 24-hr intervals from 12 to 96 hr after exposure. The resulting time course of MNC frequency was biphasic, with a clear peak 24 hr after exposure, irrespective of the kind of radiation applied and the dose used. The half-life of MNCs induced in the gill tissues by the two exposures fluctuated around 28 hr, with no significant dose-dependent trend for either X-ray- or neutron-exposed fish. As assayed 24 hr after exposure, the MNC frequency increased linearly over the control level with increasing doses of both X-rays and fast neutrons. The relative biological effectiveness (RBE) of fast neutrons to X-rays for MNC induction was estimated to be 4.3 +/- 0.6. This value is close to the RBE value of 5.1 +/- 0.3 reported for fast neutron induction of somatic crossing-over mutations in Drosophila melanogaster that arise from recombination repair of DNA double-strand breaks. These results and other data support our conclusion that the medaka gill cell micronucleus assay is a reliable short-term test for detecting potential inducers of DNA double-strand breaks.

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Development of a Biological Dosimeter for Translocation Scoring Based on Two-Color Fluorescence in Situ Hybridization of Chromosome Subsets

Cited by 8 publications

References 24 publications

Biological Effects of High-Energy Neutrons MeasuredIn VivoUsing a Vertebrate Model

Biological Effects of High-Energy Neutrons MeasuredIn VivoUsing a Vertebrate Model

Chromosome analysis of lymphocytes from radiation workers in tritium-applying industry

Dose‐ and time‐dependent responses for micronucleus induction by X‐rays and fast neutrons in gill cells of medaka (Oryzias latipes)

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