Development of a bone nonunion in a noncritical segmental tibia defect model in sheep utilizing interlocking nail as an internal fixation system

Lozada-Gallegos, Angel R; Letechipia-Moreno, Jorge; Palma‐Lara, Icela; Montero, Aldo Alessi; Rodríguez, Gerardo; Castro‐Muñozledo, Federico; Cornejo-Cortés, Miguel A.; Juárez-Mosqueda, M. L.

doi:10.1016/j.jss.2013.02.060

Cited by 21 publications

(14 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A post-surgical lameness was determined within both groups which lasted longer in animals of the LAE442 alloy group. Lameness, to a varying extend was reported by different authors using sheep model on intramedullary nailing as well [65][66][67]. In comparison to current available literature the lameness within this study appears to be more pronounced.…”

Section: Discussionsupporting

confidence: 42%

In vivo evaluation of a magnesium-based degradable intramedullary nailing system in a sheep model

et al. 2015

View full text Add to dashboard Cite

Section: Discussionsupporting

confidence: 42%

In vivo evaluation of a magnesium-based degradable intramedullary nailing system in a sheep model

et al. 2015

View full text Add to dashboard Cite

“…In fact, in the present study, some bone callus formation evidences were found in the tibia where a reduction of defect size from 10 mm to about 5 mm occurred. This outcome is in agreement with the study by Lozada‐Gallegos et al (), where the formation of bone callus was observed at 6 weeks in a 5‐mm fracture gap despite the use of a silicone ring, although they did not find any contact between the edges of the osteotomy. Therefore, in a noncritical size defect, the gap dimension seems crucial for achieve a nonunion.…”

Section: Discussionsupporting

confidence: 93%

“…However, preclinical models used for bone nonunion research frequently do not meet these criteria. Current large animal models of atrophic nonunion involve either large segmental osseous defects (Christou, Oliver, Pelletier, & Walsh, ; Den Boer et al, ; Reichert et al, ) or the interposition of foreign materials to isolate the fracture from the surrounding soft tissues in order to prevent the normal healing processes (Lozada‐Gallegos et al, ; Oni, ). None of these models simulates the clinical situation where the gap defect is small enough for fracture healing, but some local biological processes prevent union (Einhorn, ).…”

Section: Introductionmentioning

confidence: 99%

Nonunion fracture healing: Evaluation of effectiveness of demineralized bone matrix and mesenchymal stem cells in a novel sheep bone nonunion model

Dozza

Salamanna

Baleani

et al. 2018

J Tissue Eng Regen Med

View full text Add to dashboard Cite

Nonunion treatment has a high rate of success, although recalcitrant nonunion may determine the need for amputation. Therefore, new treatment options are continuously investigated in order to further reduce the risk of nonunion recurrence. This study aimed to (a) develop a new large animal model for bone atrophic nonunion and (b) compare the efficacy of demineralized bone matrix (DBM) and DBM in combination with mesenchymal stem cells (MSC) in the new nonunion model. The new model consists of a noncritical, full-thickness segmental defect created in the sheep tibia, stabilized by an intramedullary nail, and involves the creation of a locally impaired blood supply achieved through periosteum excision and electrocauterization of the stump ends. Six weeks after defect creation, lack of hard tissue callus and established nonunion was observed in all operated tibiae both by radiographic and clinical evaluation. Nonunion was treated with allogeneic DBM or autologous MSC cultivated on DBM particles (DBM + MSC) for 1 day before implantation. Twelve weeks after treatment, radiographic, microtomographic, histologic, and histomorphometric analysis showed the formation of bone callus in DBM group, whereas the fracture healing appeared at an early stage in DBM + MSC group. Torsional strength and stiffness of the DBM group appeared higher than those of DBM + MSC group, although the differences were not statistically significant. In conclusion, a new sheep bone nonunion model resembling the complexity of the clinical condition was developed. DBM is an effective option for nonunion treatment, whereas MSC do not improve the healing process when cultivated on DBM particles before implantation.

show abstract

“…Previous studies have attributed the regenerative capacity of autografts to periosteum, which accounts for about 70% of bone and cartilage formation at the initiation of autograft healing [1, 2]. Periosteal stripping is known to be associated with a high incidence of bone nonunion, whereas preservation of periosteum or use of a periosteum tube graft significantly improves bone graft incorporation and bone defect reconstruction [3-5]. …”

Section: Introductionmentioning

confidence: 99%

Layer-by-layer nanofiber-enabled engineering of biomimetic periosteum for bone repair and reconstruction

et al. 2018

View full text Add to dashboard Cite

Periosteum plays an indispensable role in bone repair and reconstruction. To recapitulate the remarkable regenerative capacity of periosteum, a biomimetic tissue-engineered periosteum (TEP) was constructed via layer-by-layer bottom-up strategy utilizing polycaprolactone (PCL), collagen, and nano-hydroxyapatite composite nanofiber sheets seeded with bone marrow stromal cells (BMSCs). When combined with a structural bone allograft to repair a 4 mm segmental bone defect created in the mouse femur, TEP restored donor-site periosteal bone formation, reversing the poor biomechanics of bone allograft healing at 6 weeks post-implantation. Further histologic analyses showed that TEP recapitulated the entire periosteal bone repair process, as evidenced by donor-dependent formation of bone and cartilage, induction of distinct CD31 type H endothelium, reconstitution of bone marrow and remodeling of bone allografts. Compared to nanofiber sheets without BMSC seeding, TEP eliminated the fibrotic tissue capsule elicited by nanofiber sheets, leading to a marked improvement of osseointegration at the compromised periosteal site. Taken together, our study demonstrated a novel layer-by-layer engineering platform for construction of a versatile biomimetic periosteum, enabling further assembly of a multi-component and multifunctional periosteum replacement for bone defect repair and reconstruction.

show abstract

Development of a bone nonunion in a noncritical segmental tibia defect model in sheep utilizing interlocking nail as an internal fixation system

Cited by 21 publications

References 60 publications

In vivo evaluation of a magnesium-based degradable intramedullary nailing system in a sheep model

In vivo evaluation of a magnesium-based degradable intramedullary nailing system in a sheep model

Nonunion fracture healing: Evaluation of effectiveness of demineralized bone matrix and mesenchymal stem cells in a novel sheep bone nonunion model

Layer-by-layer nanofiber-enabled engineering of biomimetic periosteum for bone repair and reconstruction

Contact Info

Product

Resources

About