Development of a broad-spectrum monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for the multi-residue detection of avermectins in edible animal tissues and milk

Ni, Tengteng; Peng, Dapeng; Wang, Yanxin; Pan, Yuanhu; Xie, Shuyu; Chen, Dongmei; Wang, Yulian; Tao, Yanfei; Zhang, Yuan

doi:10.1016/j.foodchem.2019.02.011

Cited by 45 publications

(24 citation statements)

References 35 publications

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“…A cross-reactivity (CR) study was performed to determine the specificity of this mAb. 23 DAS, T-2, HT-2, DON, and NIV were subjected to ic-ELISA. The CR values were calculated by the following formula: …”

Section: Methodsmentioning

confidence: 99%

Development of an ic-ELISA and Immunochromatographic Strip Assay for the Detection of Diacetoxyscirpenol in Rice

Jin

Cui

et al. 2020

ACS Omega

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Diacetoxyscirpenol (DAS) is a highly toxic type A trichothecene, a natural contaminant in food and animal feed, which is a serious hazard to human and animal health. An anti-DAS mAb, 3H10, with high sensitivity and specificity, was prepared, and an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a lateral-flow immunochromatographic strip (ICA strip) were developed for rapid testing of DAS in rice samples. The 50% inhibitory concentration and limit of detection of ic-ELISA were 5.97 and 0.78 ng/mL, respectively. The recovery of rice samples ranged from 99.4 to 110.7%, demonstrating that the analytical method can be used to detect rice samples. The cutoff value of the lateral-flow ICA strip to DAS was 500 ng/g. The developed immunoassay method can provide an effective method of initially detecting and screening DAS in food and feed samples.

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Section: Methodsmentioning

confidence: 99%

Development of an ic-ELISA and Immunochromatographic Strip Assay for the Detection of Diacetoxyscirpenol in Rice

Jin

Cui

et al. 2020

ACS Omega

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“…Indirect competitive immunoassay, containing IC-ELISA and IC-TRFIA which were the most common method for the small molecule hazards (such as small molecule pesticides, biotoxins and antibiotics, etc.) determination (Kavanagh, Elliott, & Campbell, 2015;Ni et al, 2019;Watanabe, Miyake, & Yogo, 2013). Because of its superiority, the key evaluation index of antibody to antigen, containing IC 10 (LOD), IC 50 and IC 20 -IC 80 (linear working range), which could be calculated out directly.…”

Section: Ic-trfia Establishment Of Van and Its Analoguesmentioning

confidence: 99%

Establishment of an ultrasensitive indirect competitive time-resolved fluoroimmunoassay for vancomycin determination

Han

Dong

et al. 2019

Food and Agricultural Immunology

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Vancomycin (Van) is a common antibiotic that pollutes food, feed and ecological environment. In the present study, an anti-Van polyclonal antibodies (pAbs) and Eu 3+-labeled goat anti-rabbit IgG monoclonal antibody based indirect competitive timeresolved fluoroimmunoassay (IC-TRFIA) was established for ultrasensitive determination of Van, and its half-maximum inhibition concentrations (IC 50) was 13.74 ng/L and the limit of detection (LOD) was 1.07 ng/L. The IC-TRFIA showed strong crossreactivity (CRs) of 36.2% for norvancomycin, and less than 0.1% for polymyxin B, kanamycin and ampicillin. It was found that the IC-TRFIA for Van spiked in different milk powder samples determination were with good accuracy, stability and repeatability, and its recoveries are 83.0-97.9%, also with its coefficient of variations (CVs) are 3.0-12.9% in intra-assay and inter-assay. The results showed that the established IC-TRFIA was promising for ultrasensitive determination of Van in food samples. Highlights (1) Prepared high-quality Van-KLH/BSA conjugates. (2) Obtained high activity and purify anti-Van polyclonal antibodies. (3) Prepared high-quality Eu 3+-labelled goat anti-rabbit IgG monoclonal antibody. (4) Firstly established an IC-TRFIA for Van based on the anti-Van polyclonal antibodies and Eu 3+-labelled goat anti-rabbit IgG monoclonal antibody. (5) The IC-TRFIA can be used for ultrasensitive determination of Van in milk powder samples.

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“…The approaches include high-performance liquid chromatography-ultraviolet detection (HPLC-UV) 4 , liquid chromatography-tandem mass spectrometry (LC-MS) 6 , high-performance liquid chromatographyuorescent detector (HPLC-FLD) 7 , enzyme-linked immunosorbent assay (ELISA) 8 and liquid chromatography-tandem mass spectrometry/mass spectrometry (HPLC-MS/MS) 9 . However, these approaches either needed a time-consuming process, high-cost accurate instrument, or high professional operators, which makes them di cult to apply in general laboratories 10 . Herein, considering the potential harm of abamectin B 1 for public health, a convenient and accurate analysis method for residual abamectin B 1 is necessary 10,11 .…”

Section: Introductionmentioning

confidence: 99%

“…However, these approaches either needed a time-consuming process, high-cost accurate instrument, or high professional operators, which makes them di cult to apply in general laboratories 10 . Herein, considering the potential harm of abamectin B 1 for public health, a convenient and accurate analysis method for residual abamectin B 1 is necessary 10,11 . Therefore, it is necessary to explore a convenient analysis method for detection of residual abamectin B 1 to reduce the potential harm to public health.…”

Section: Introductionmentioning

confidence: 99%

A highly sensitive octopus-like azobenzene fluorescent probe for determination of abamectin B1 in apples

Guo

et al. 2020

Preprint

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The development of detecting residual level of abamectin B1 in apples is of great importance to public health. Herein, we synthesized a octopus-like azobenzene fluorescent probe 1,3,5-tris (5'-[(E)-(p-phenoxyazo) diazenyl)] benzene-1,3-dicarboxylic acid) benzene (TPB) for preliminary detection of abamectin B1 in apples. The TPB molecule was characterized by ultraviolet-visible absorption spectrometry, 1H-nuclear magnetic resonance, fourier-transform infrared (FT-IR), electrospray ionization mass spectroscopy (ESI-MS) and fluorescent spectrum. A proper determination condition was optimized, with limit of detection and limit of quantification of 1.3 µg L-1 and 4.4 μg L-1, respectively. The mechanism of this probe to identify abamectin B1 was illustrated in terms of undergoing aromatic nucleophilic substitution, by comparing fluorescence changes, FT-IR and ESI-MS. Furthermore, a facile quantitative detection of the residual abamectin B1 in apples was achieved. Good reproducibility was shown based on relative standard deviation of 2.20%. Six carboxyl recognition sites, three azo groups and unique fluorescence signal towards abamectin B1 of this fluorescent probe decided ideal sensitivity, specificity and selectivity. The results show that the octopus-like azobenzene fluorescent probe may be promising for evaluating abamectin B1 in agricultural foods.

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Development of a broad-spectrum monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for the multi-residue detection of avermectins in edible animal tissues and milk

Cited by 45 publications

References 35 publications

Development of an ic-ELISA and Immunochromatographic Strip Assay for the Detection of Diacetoxyscirpenol in Rice

Development of an ic-ELISA and Immunochromatographic Strip Assay for the Detection of Diacetoxyscirpenol in Rice

Establishment of an ultrasensitive indirect competitive time-resolved fluoroimmunoassay for vancomycin determination

A highly sensitive octopus-like azobenzene fluorescent probe for determination of abamectin B1 in apples

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