Development of a broodstock diet to improve developmental competence of embryos in European eel,Anguilla anguilla

Abstract

We examined the effect of dietary arachidonic acid (ARA) and eicosapentaenoic acid (EPA) on the production of embryos and hatched larvae in the European eel, Anguilla anguilla. Two diets with high and intermediate levels of ARA and low and intermediate levels of EPA (Feed 1: ARA 1.9%, EPA 4.2%; Feed 2: ARA 1.2%, EPA 5.1% of total fatty acids) were tested against a commercial diet (DE: ARA: 0.5%, EPA: 8.2% of total fatty acids). After 24 weeks of feeding, ARA levels in the muscles and ovaries increased to 0.9% and 1.3% of total fatty acids, respectively, in Feed 1 and were significantly higher than in Feed 2 and DE. Female broodstock was not fed during hormonal treatment to induce vitellogenesis and ovulation. EPA levels in females fed the test diets decreased in the both muscle and ovary and were significantly lower in eggs from females fed Feed 1. The highest percentage of stripped females, producing viable eggs and larvae, were those females fed the highest dietary ARA levels (Feed 1). The level of lipid peroxidation products in eggs was similar among treatment, indicating that the lowest dietary levels of vitamin C and vitamin E were sufficient. In the unfertilized eggs, ARA levels were also highest (1.1% of total fatty acids) in the diet with highest ARA levels (Feed 1).

Dietary amino acids impact sperm performance traits for a catadromous fish, Anguilla anguilla reared in captivity

“…Previous studies have investigated dietary impacts on egg quality (Heinsbroek et al, 2013;Støttrup et al, 2013Støttrup et al, , 2016. Based on these findings, fatty acid composition of the best performing diet for females was used as basis for the fatty acid composition of these tested diets (hereafter EH-4, EH-5, and EH-6).…”

“…During the last decade, different strategies have been employed to improve European eel gamete quality, especially by conducting studies on reproductive performance and broodstock diets at specific developmental windows (Støttrup et al, 2013;Baeza et al, 2014Baeza et al, , 2015aButts et al, 2015;da Silva et al, 2016). For example, supplementation of broodstock diets with lipids [mainly polyunsaturated fatty acid (PUFA)], improved spermiation and milt quality in males (Butts et al, 2015), and oocyte growth and ovarian development in females (Støttrup et al, 2016;da Silva et al, 2016). Therefore, further information on the effects of broodstock diets on European eel gamete performance could enhance the process of domestication for mass production of larvae.…”

Assessment of lipid uptake and fatty acid metabolism of European eel larvae (Anguilla anguilla) determined by 14C in vivo incubation

“…Upon returning to the Sargasso Sea spawning area, silver eels likely cease feeding and undergo maturation while using freshwater obtained fat resources, as was reported for migrating Japanese silver eels (Saito et al, 2015). Not only tissue levels of fat seems of importance, but muscle, visceral fat and ovary of wild female silver eels have revealed significantly higher ARA content than farmed eels fed standard commercial fish meal based diet (Støttrup et al, 2013) and evidence of the significance of supplemented ARA in the reproductive success of farmed European and Japanese eels has been documented (Furuita et al, 2006;Støttrup et al, 2016;Kottmann et al, 2020b). These findings moreover suggest a significant importance of ARA for first-feeding of European eel larvae, although this has been questioned in Japanese pre-leptocephalus larvae for which a low lipid diet (i.e.…”

The Presence of Bioactive Compounds in European Eel (Anguilla anguilla) Skin: A Comparative Study with Edible Tissue

“…European eel ( Anguilla anguilla ), a teleost fish belonging to the family Anguillidae , is a commercially valuable species in Europe and Asia. As a result of recent overfishing on coasts and several biological concerns [ 22 ], great attention has been accorded to the development of this fish species as a farmed product [ 23 , 24 ]. With the extension of farming, the total annual production is assumed to be over 10,500 tonnes, The Netherlands consuming ca.…”