Development of a calibration and standardization procedure for LA-ICP-MS using a conventional ink-jet printer for quantification of proteins in electro- and Western-blot assays

Atomic spectrometry update: review of advances in atomic spectrometry and related techniques

“…The problem of measuring Cu and Fe isotope compositions on samples with low concentrations of these elements has been tackled by Costas-Rodriguez et al 156 Essentially a standard addition principle was used in mixing a known isotope ratio solution with the sample solution, both of known Cu concentration. Resulting mixed d 65 Cu values for the sample agreed with the Cu isotope ratio determined by conventional bracketing techniques at higher sample concentration.…”

“…LODs in the range of hundreds of ng g À1 were achieved. Another approach to internal standardisation and calibration for bioimaging using LA-ICP-MS was proposed by Hoesl et al 65 The procedure was based on printing metal spiked inks onto the top of thin layer samples using an ink-jet printer. The ink was spiked with indium as an internal standard and homogenously printed over the entire membrane prior to LA-ICP-MS detection.…”

“…Cu and Fe isotopes were separated in a single-step chromatographic procedure by Liu et al 157 prior to MC-ICP-MS analysis. The authors documented a long-term reproducibility of better than AE0.05& for both d 65 Cu and d 56 Fe and used the procedure to evaluate the Fe and Cu isotopic characteristics of igneous rock standards. Fe isotopes of these rocks were found to be relatively uniform whereas d 65 Cu had a range of 0.4&.…”

A new method for the SI-traceable quantification of element contents in solid samples using LA-ICP-MS

“…Furthermore, Hoesl et al presented the alternative to print some spiked inks onto the sample and the matrix-matched standards, respectively. 22 With this method an internal standardisation and a calibration can take place in combination whereat a homogeneous printing is a main condition in this concept. 22 The calibration with a matrix-matched standard shows a lot of different ways and offers a good opportunity for a quantitative analysis, but oen a suitable CRM is not available and due to the complexity of the sample it is not possible to prepare a perfect reference material.…”

Quantitative aspects of inductively coupled plasma mass spectrometry

“…Probably owing to this feature, the literature reports many custom-developed matrix-matched calibration approaches, which should fit to various applications of LA-ICP-MS in quantitative elemental analysis [54][55][56][57][58]. The most often reported calibration approach is based on the use of Standard Reference Material (SRM) NIST 610 glass, which is available from the National Institute of Standards and Technology (NIST).…”