Development of a canine model of pulmonary emphysema and imaging of the emphysematous lung with infrared thoracoscopy

Gotoh, Masashi; Okamoto, Taku; Yamamoto, Yasumichi; Liu, Dage; Kameyama, Kotaro; Hayashi, Eiichi; Huang, Cheng‐Long; Yokomise, Hiroyasu

doi:10.1016/s0022-5223(03)00807-9

Cited by 18 publications

(14 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…4 Briefly, a bronchoscope was inserted into the bronchial system of each of three experimental beagle dogs weighing 10-11 kg that had been anesthetized by intramuscular injection of 125 mg/kg ketamine (Ketalar®, Sankyo Co., Ltd., Tokyo, Japan) and 5.0 mg/kg xylazine (Selactal®, Byer Ltd., Tokyo, Japan). A balloon catheter (Edwards Swan-Ganz, Baxter Health care Co., CA) was inserted through the channel port of the bronchofiberscope into the left lingular segmental bronchus, and the bronchus was occluded by inflating the balloon for 15 minutes.…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, the emphysematous lung did not contain ICG because of its poor blood supply. 4 After intramuscular injection of 125 mg/kg ketamine and 5.0 mg/kg xylazine, an endotracheal tube with a bronchial blocker (Univent tube, Fuji System Co., Tokyo, Japan) was inserted into the trachea of each of the three emphysema model dogs. Anesthesia was maintained during surgery by inhalation anesthesia with sevoflurane, oxygen, and dinitrogen monoxide.…”

Section: Methodsmentioning

confidence: 99%

“…We then administered 2.5 mg/kg ICG (Diagnogreen, Daiichi Pharmaceutical Co., Ltd., Tokyo, Japan) by bolus intravenous injection. 4 The thoracoscopic image was recorded digitally throughout the surgery, and static images were captured from the digital video when the time of sharpest color contrast, determined subjectively by the naked eye, occurred between the emphysematous and normal regions.…”

Section: Methodsmentioning

confidence: 99%

“…3 Previously, we reported that infrared thoracoscopy (IRT) with intravenous injection of indocyanine green (ICG) was useful for detecting emphysematous regions. 4 Here, we analyzed IRT images, quantified the degree of emphysema, and examined whether we could use such analyzed images as guides for accurately removing target areas.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Real Time Imaging and Quantitative Evaluation of the Emphysematous Lung by Infrared Thoracoscopy in Experimental Dogs

et al. 2005

Self Cite

View full text Add to dashboard Cite

To accurately excise emphysematous lung tissue in volume reduction surgery, we projected a digitally analyzed excision line on the real time image obtained by infrared thoracoscopy (IRT) with indocyanine green (ICG) intravenous injection. Emphysema was created in the canine lung by intrabronchial injection of elastase. We examined the emphysematous lung by IRT after intravenous injection of ICG. A digitized static image was obtained and analyzed in real time during the surgery. The color densities on the image were measured and the color density ratios (CDRs) calculated. We resected lung areas where the CDR was 1.0 or less. Resected and residual lung areas were examined microscopically. Microscopically, areas displayed as white by IRT with intravenous injection of ICG were emphysematous, and areas displayed as blue were normal. Areas with a CDR of 1.0 or less were emphysematous, and we were able to determine an appropriate excision line by connecting sample points with a CDR of 1.0. Use of digital image analysis combined with IRT after ICG injection enabled us to remove emphysematous regions accurately.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 2 more Smart Citations

Real Time Imaging and Quantitative Evaluation of the Emphysematous Lung by Infrared Thoracoscopy in Experimental Dogs

et al. 2005

Self Cite

View full text Add to dashboard Cite

show abstract

“…Failure to detect all the bullous lesions maybe one of the reasons for recurrence. Gotoh et al developed a method to observe pulmonary emphysematous lesions in a canine model (15). ICG was injected through peripheral vein.…”

Section: Detection Of Bullous Lesionsmentioning

confidence: 99%

Applications of indocyanine green based near-infrared fluorescence imaging in thoracic surgery

et al. 2016

View full text Add to dashboard Cite

Near-infrared (NIR) fluorescence guided surgery is an emerging technique. This technique uses the combination of dyes and NIR imaging devices to expand the visible spectrum. Thus it can provide more anatomic and functional information, and may facilitate a more complete resection of cancer, or better protection of important normal structures. Recently, significant progress has been made in the field of NIR fluorescence guided thoracic surgery. This may lead to better prognosis and health-economic outcomes.In this article, the current studies of indocyanine green (ICG) based NIR fluorescence guided thoracic surgeries are reviewed. The applications are classified into four categories, which are applications based on blood supply, lymphatic drainage, the enhanced permeability and retention (EPR) effect, and the other mechanisms.Keywords: Near-infrared fluorescence; indocyanine green (ICG); blood supply; lymphatic drainage; enhanced permeability and retention effect (EPR effect)

show abstract

Wingless signaling in a large insect, the blowfly Lucilia sericata: A beautiful example of evolutionary developmental biology

Mellenthin

Fahmy

Ali

et al. 2005

Developmental Dynamics

View full text Add to dashboard Cite

Blowflies are the primary facultative agent in causing myiasis of domestic sheep in the whole world and, at the same time, it is an important tool for forensic medicine. Surprisingly, and in contrast to its importance, almost no data regarding the embryology and molecular markers are known for this insect. In this report, we present a detailed description of the blowfly Lucilia sericata embryogenesis and of imaginal disc development. The embryogenesis of Lucilia strongly resembles that of Drosophila, despite their apparent size difference. Moreover, imaginal disc development appears to be equally well conserved. Through cloning, expression, and functional studies, we show that the Lucilia Wingless (Wg) protein is highly conserved between the two species. We further show that parasegments are established in Lucilia, however, engrailed expression shows a more dynamic expression pattern than expected in comparison to Drosophila. Over-expression of Lucilia Wingless in Drosophila shows wingless-like wing phenotypes, suggesting that Lucilia Wingless blocks the signalling activity of Drosophila Wingless. Upon injection of wg dsRNA, we observe a "lawn of denticle" phenotype, closely resembling that of Drosophila. Due to the large size of the insect, the distance over which Wingless exerts signalling activity is up to three times larger than in Drosophila, yet the consequences are very similar. Our data demonstrate long-range wingless signaling mechanisms adapted for patterning large domains of naked cuticle and suggest signaling properties of Lucilia Wingless that are distinct from those of Drosophila Wingless. Developmental Dynamics 235:347-360, 2006.

show abstract

Development of a canine model of pulmonary emphysema and imaging of the emphysematous lung with infrared thoracoscopy

Abstract: We developed a successful canine model of pulmonary emphysema by injecting porcine pancreatic elastase. Infrared thoracoscopic examination revealed that the staining of emphysematous lung using indocyanine green injection was significantly greater than of normal lung.

Cited by 18 publications

References 11 publications

Real Time Imaging and Quantitative Evaluation of the Emphysematous Lung by Infrared Thoracoscopy in Experimental Dogs

Real Time Imaging and Quantitative Evaluation of the Emphysematous Lung by Infrared Thoracoscopy in Experimental Dogs

Applications of indocyanine green based near-infrared fluorescence imaging in thoracic surgery

Wingless signaling in a large insect, the blowfly Lucilia sericata: A beautiful example of evolutionary developmental biology

Contact Info

Product

Resources

About