Development of a cellular biosensor for the detection of 2,4,6-trichloroanisole (TCA)

Biosensor-Based Approaches for Detecting Ochratoxin A and 2,4,6-Trichloroanisole in Beverages

“…In turn, this change can be measured with established electrophysiological techniques, in particular the practically applicable bioelectric recognition assay (BERA) [72][73][74], a method based on the measurement of the bioelectric properties of a cluster of cells, usually gel-immobilized. Under the experimental conditions applied in [68], a very low limit of detection (0.1 ppt) was achieved for TCA, a value that is lower than the human sensory threshold concentration. It was shown that the response of membrane-engineered cells to increasing TCA concentrations was partly due to the depletion of their intracellular Ca 2+ stores, while the overall assay was completed in just five minutes.…”

“…Consequently, this achievement could represent a disruptive change in cork-quality screening. However, a possible limitation of the BERA- The same PAb developed by Sanvicens et al has been utilized in yet another, disruptive biosensor-based approach, originally developed by Varelas et al [68]. In this case, cellular biorecognition elements with a highly selective response against TCA were created by the osmotic insertion of PAb 76 into the membrane of immortalized hamster adult kidney (HaK) cells, immobilized in an alginate gel matrix.…”

“…A considerable improvement of the BERA-based approach was reported by Apostolou et al [75] who modified the protocol of Varelas et al [68] by electroinserting (instead of osmotically handling) Monkey African green kidney (Vero) cells with the same antibody (PAb 76 ). In addition, cells were non-immobilized (i.e., in suspension) so that direct contact with the potential measuring electrode was established (Figure 4).…”

Biosensors Applications in Agri-food Industry

“…This is the case of the 2,4,6-trichloroanisole, a compound (Varelas et al, 2011) related to corks in wine bottles causing considerable losses to the wine industry. Varelas et al (2011) developed a rapid novel biosensor system based on a bioelectric recognition assay.…”

“…This is the case of the 2,4,6-trichloroanisole, a compound (Varelas et al, 2011) related to corks in wine bottles causing considerable losses to the wine industry. Varelas et al (2011) developed a rapid novel biosensor system based on a bioelectric recognition assay. The sensor measured the electric response of cultured membraneengineered fibroblast cells suspended in an alginate gel matrix due to the change of their membrane potential in the presence of the analyte.…”