Development of a chemiluminescence-based quantitative lateral flow immunoassay for on-field detection of 2,4,6-trinitrotoluene

Mirasoli, Mara; Buragina, Angela; Dolci, Luisa Stella; Guardigli, Massimo; Simoni, Patrizia; Montoya, Ángel; Maiolini, Elisabetta; Girotti, Stefano; Roda, Aldo

doi:10.1016/j.aca.2012.01.036

Cited by 66 publications

(35 citation statements)

References 35 publications

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“…In this type of LFA, the antibody-antigen binding reactions and enzymatic reactions occur simultenously. The enzymatic reaction generated CL signal and this CL emissions are measured by using a CCD camera [223].…”

Section: Enzymesmentioning

confidence: 99%

Lateral flow assays: Principles, designs and labels

Bahadır

Sezgintürk

2016

TrAC Trends in Analytical Chemistry

498

315

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Section: Enzymesmentioning

confidence: 99%

Lateral flow assays: Principles, designs and labels

Bahadır

Sezgintürk

2016

TrAC Trends in Analytical Chemistry

498

315

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“…Their coupling with CL detec-tion has previously shown to improve analytical performance, including a higher sensitivity and the ability to provide quan-titative information [15][16][17][18]. This would therefore bring the range of applications as well as the analytical performance of LFIAs closer to that of conventional analytical instrumenta-tion.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, the intensity of illumination is also very important to achieve the best detect-ability, especially for fluorescence-based assays, and this requires a large light power that hampers portability [14]. To this end, light imaging systems based on a charge-coupled-device (CCD) camera [15][16][17] or even a smartphone's complemen-tary metal-oxide semiconductor (CMOS) sensor [18] was employed for photons detection in CL-LFIAs.…”

Section: Introductionmentioning

confidence: 99%

Chemiluminescence lateral flow immunoassay cartridge with integrated amorphous silicon photosensors array for human serum albumin detection in urine samples

et al. 2016

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A novel and disposable cartridge for chemilumi-nescent (CL)-lateral flow immunoassay (LFIA) with integrat-ed amorphous silicon (a-Si:H) photosensors array was devel-oped and applied to quantitatively detect human serum albu-min (HSA) in urine samples. The presented analytical method is based on an indirect competitive immunoassay using horse-radish peroxidase (HRP) as a tracer, which is detected by adding the luminol/enhancer/hydrogen peroxide CL cocktail. The system comprises an array of a-Si:H photosensors depos-ited on a glass substrate, on which a PDMS cartridge that houses the LFIA strip and the reagents necessary for the CL immunoassay was optically coupled to obtain an integrated analytical device controlled by a portable readout electronics. The method is simple and fast with a detection limit of 2.5 mg L −1 for HSA in urine and a dynamic range up to 850 mg L −1 , which is suitable for measuring physiological levels of HSA in urine samples and their variation in different diseases (micro-and macroalbuminuria). The use of CL de-tection allowed accurate and objective analyte quantification in a dynamic range that extends from femtomoles to pico-moles. The analytical performances of this integrated device were found to be comparable with those obtained using a charge-coupled device (CCD) as a reference off-chip detector. These results demonstrate that integrating the a-Si:H photosensors array with CL-LFIA technique provides com-pact, sensitive and low-cost systems for CL-based bioassays with a wide range of applications for in-field and point-of-care bioanalyses.

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“…25, 26 Similarly, alternate methods to modulate the flow of reporters through the strip have been proposed, including electrophoresis, 27 isotachophoresis, 28 and flow delay. 29 Finally, proposed ultrasensitive readouts include volumetric magnetization, 30, 31 near-infrared detection, 32 surface-enhanced Raman spectroscopy, 33 fluorescence, 34, 35 chemiluminescence, 9, 36 phosphorescence 37 , and thermal contrast. 38 In addition, much recent work has focused on smartphone-compatible readouts 39, 40 that are suitable for settings lacking sophisticated laboratory infrastructure or instrumentation.…”

Section: Introductionmentioning

confidence: 99%

Orientational binding modes of reporters in a viral-nanoparticle lateral flow assay

Kim

Poling-Skutvik

Trabuco

et al. 2017

Analyst

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Using microscopy and image analysis, we characterize binding of filamentous viral nanoparticles to a fibrous affinity matrix as models for reporter capture in a lateral flow assay (LFA). M13 bacteriophage (M13) displaying an in vivo-biotinylated peptide (AviTag) genetically fused to the M13 tail protein p3 are functionalized with fluorescent labels. We functionalize glass fiber LFA membranes with antibodies to M13, which primarily capture M13 on the major p8 coat proteins, or with avidin, which captures M13 at the biotin-functionalized tail, and compare orientational modes of reporter capture for the side- versus tip-binding recognition interactions. The number of captured M13 is greater for side-binding than for tip-binding, as expected from the number of recognition groups. Whereas two-thirds of side-bound M13 captured by an anti-M13 antibody bind immediately after colliding with the membrane, tip-bound M13 prominently exhibit three additional orientational modes that require M13 to reorient to enable binding. These results are consistent with the idea that the elongated M13 shape couples with the complex flow field in an open and disordered fibrous LFA membrane to enhance capture.

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Development of a chemiluminescence-based quantitative lateral flow immunoassay for on-field detection of 2,4,6-trinitrotoluene

Cited by 66 publications

References 35 publications

Lateral flow assays: Principles, designs and labels

Lateral flow assays: Principles, designs and labels

Chemiluminescence lateral flow immunoassay cartridge with integrated amorphous silicon photosensors array for human serum albumin detection in urine samples

Orientational binding modes of reporters in a viral-nanoparticle lateral flow assay

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