Development of a chemiluminescent enzyme‐linked immunosorbent assay for five sulfonamide residues in chicken muscle and pig muscle

Abstract

BACKGROUND: An enzyme-linked immunosorbent assay (ELISA) based on polyclonal antibodies with enhanced chemiluminescent (ECL) detection of sulfonamides in food samples has been optimised and characterised. The specificity of the assay was assessed by determining cross-reactivities with a set of 16 sulfonamides. The aim of this study was to develop a method for determining sulfonamides with high sensitivity.

The matrix interference to the immunoassay of food samples: The effect of some proteins in aquatic products

“…The complex and variable chemical components in food samples might greatly interfere with the immunological reactions, resulting in significantly reduced accuracy and sensitivity, and even false results (Mitchell & Lowe, 2009;Tiwari et al, 2010;Zhang et al, 2007). In past years such matrix interference has been reported in a wide range of food samples, including vegetable, tea, fruit, chocolate, dairy, nuts, confectionery, shellfish, animal muscles and fishes (Liu, Fang, Zhang, Zheng, & Wang, 2009;Naar et al, 2002;Tate & Ward, 2004). However, due to very limited information about the mechanism of the matrix effect, till now it still seems difficult to develop reasonable and simple techniques to solve the problem.…”

Highly broad-specific and sensitive direct competitive enzyme-linked immunosorbent assay for screening multi-antibacterial synergists: assay optimization and application to animal-derived food

“…Matrix effects are a common challenge for immunoassays in food analysis and can be removed in a number of ways. Dilution is a commonly adopted and effective method to remove matrix effects (Liu, Fang, Zhang, Zheng, & Wang, 2009). To study possible matrix effects from milk, egg, chicken and pork, standard curves were prepared with these samples and with assay buffer as a control.…”

A sensitive, rapid chemiluminescence ELISA for the detection of 1, 4-bisdesoxycyadox residue in chicken muscle and liver

“…Therefore, the extracts of the fortied samples were diluted at least 10-fold prior to analysis, indicating that the proposed method can provide accurate quantication for those samples needing dilution prior to analysis. 30…”