Development of a chimeric aptamer and an AuNPs aptasensor for highly sensitive and specific identification of Aflatoxin B1

Recent Progress on Techniques in the Detection of Aflatoxin B1 in Edible Oil: A Mini Review

“…Yang et al [ 226 ] first devised a selection method based on rational truncation and post-splicing and developed a bivalent anti-AFB 1 chimeric aptamer (B72) that was measured by micro-thermophoresis (MST) compared to the initial selection. The affinity of the anti-AFB 1 aptamer (B50) increased by 188-fold, and the study also found that B72 has a dual binding site for AFB 1 , which is consistent with the experimental results obtained by isothermal titration calorimetry (ITC) and molecular docking simulations.…”

A highly sensitive competitive aptasensor for AFB1 detection based on an exonuclease-assisted target recycling amplification strategy

“…The secondary structure of the AFB 1 aptamer was predicted by the Mfold web server 23 and then its tertiary structures were obtained from Xiao Lab (http://biophy.hust.edu.cn/new/). The binding energy of the aptamer with AFB 1 was estimated using the AutoDockTools (ADT) 1.5.7 package.…”

“…Molecular docking could simulate aptamer-ligand interactions and completely characterize complexes, 23 allowing for generating accurate three-dimensional (3D) models and the elucidation of detailed binding sites. 24 Ma et al 25 used molecular docking to predict the critical bases of aptamer binding to chloramphenicol, and then trimmed the 80-base aptamer to 30 bases for designing the biosensor.…”

A Novel Electrochemical Method for the Sensitive Determination of Aflatoxin B1 Using a Bivalent Binding Aptamer‐cDNA Structure

“…The limit of detection (LOD) toward AFB 1 was considered to be 0.1 ng/mL (0.32 nM) (S/N = 3). In comparison to other methods for AFB 1 detection, the proposed sensing approach has better or comparable features (Table 1) [24][25][26][27][28][29][30][31][32][33].…”