Development of a colorimetric assay for quantification of favipiravir in human serum using ferrihydrite

Moriiwa, Yukiko; Oyama, Natsu; Otsuka, Ryo; Morioka, Kazuhiro; Shoji, Atsushi; Yanagida, Akio

doi:10.1016/j.talanta.2022.123827

Cited by 8 publications

(3 citation statements)

References 34 publications

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“…The percentage recovery estimated accuracy in terms of closeness of means. The molar ratio method helped determine the reaction stoichiometry by calculating the ratio of moles of reactants needed to develop unit absorbance [12].…”

Section: Methods Validationmentioning

confidence: 99%

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Novel colorimetric approach for amikacin estimation in pure powder and its pharmaceutical formulations

Mangam¹,

Sarella²,

Siddhantapu³

et al. 2023

World J. Bio. Pharm. Health Sci.

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A sensitive and validated colorimetric method was developed and optimized for the estimation of amikacin sulfate in pure and pharmaceutical dosage forms. Ascorbic acid was used as a chromogenic reagent to produce a stable color complex with amikacin that absorbs strongly at 390nm and 540nm. Several experimental factors were evaluated to maximize color development and stability, including solvent, reagent concentration, reaction time, and temperature. Optimal conditions were found using DMSO solvent, 0.2% ascorbic acid, and 40 minutes reaction time at 25°C. Under these conditions, Beer's law was obeyed in the range of 40-200μg/mL with high correlation coefficients, indicating excellent linearity. The method was validated in terms of linearity, accuracy, precision, detection/quantitation limits, and application to actual samples as per ICH guidelines. Recovery studies showed nearly 101% accuracy. Low relative standard deviations reflected high precision. Limits of detection/quantitation were 19-57μg/mL, enabling reliable analysis even at low concentrations. The method quantified amikacin content in injections, giving 98-102% accuracy. Statistical comparisons to a validated method gave no significant differences, confirming this approach provides equivalent results.

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Section: Methods Validationmentioning

confidence: 99%

“…To each tube, 0.4 ml of ascobic acid solution (5.0 x 10 -3 M) and DMSO were added to make the final volume 10 ml. The reaction mixtures were heated for 40 minutes and absorbance measured at 540 nm and 390 nm against the blank [12].…”

Section: Stochiometry Of the Color Reactionmentioning

confidence: 99%

Novel colorimetric approach for amikacin estimation in pure powder and its pharmaceutical formulations

Mangam¹,

Sarella²,

Siddhantapu³

et al. 2023

World J. Bio. Pharm. Health Sci.

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show abstract

“…Finding the genes and pathways involved in angiogenesis can be done quickly and quantitatively using the tube formation assay [50]. When exposed to angiogenic signals, endothelial cells continue to divide and migrate at a high rate.…”

Section: Tube Forming Assaymentioning

confidence: 99%

Development and Evaluation of Potato Starch and Chitosan Modified Capecitabine Nanoparticles for Enhanced Colon Cancer Treatment: A Comprehensive Study on PhysicalProperties, In vitro Efficacy, and In vivo Targeting

Bhattacharya,

Page,

Shinde

2024

Preprint

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This study explores the innovative application of carbohydrate-based nanoparticles in aggressive colon cancer treatment. We synthesized Capecitabine (CTB) nanoparticles by conjugating them with potato starch and chitosan, utilizing the unique properties of these materials. CTB nanoparticles were meticulously fabricated through ultrasonication, hydrolysis with 0.1M NaOH, and ionotropic gelation. Characterization included drug loading, rheological studies, Surface-Enhanced Raman Spectroscopy (SERS), Fourier-Transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), X-ray Diffraction (XRD), and Thermogravimetric Analysis (TGA). Analysis of XRD and FTIR spectra revealed molecular disorder and crystallinity loss. Beyond physical characterization, we explored functional and antioxidant properties, showing promise with a mean particle size of 245±10.45nm, zeta potential +30.3±8.09 mV, leading efficacy 76.45±3.85%, and 92.25±5.78% drug release over 100 hours. Subsequently, we evaluated cytotoxicity against HT-29 cells in vitro and in vivo using N, N-dimethylhydrazine-induced male Balb/c mice. Capecitabine-loaded potato starch-chitosan (CTB-PS-CS) nanoparticles exhibited superior colon histology and lower tumour scores, highlighting their robust anticancer activity. CTB-PS-CS-NPs significantly reduced VEGD and CD31 compared to free CTB. We conducted various cellular assays, including tube-forming, wound-healing, crystal violet staining, and apoptosis studies, consistently demonstrating pronounced anticancer effects on HT-29 cells. Our study supports the enhanced anticancer activity of CTB-PS-CS-NPs, indicating their potential for assessing nanoparticle efficacy and compatibility across diverse cancer models.

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Switch from The Traditional RPLC Method to the Green RPLC Methods in The Determination of The Ionization Constant (pK_a) of Favipiravir

Daldal

2024

ChemistrySelect

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In this study, the ionization constant (pKa) of favipiravir was determined using both green and the traditional RPLC methods. To determine these values, acetonitrile‐water and methanol‐water mixtures were used in the traditional RPLC methods, while ethanol‐water and micellar solvent‐free mixtures were used in the green RPLC methods. The pKa value of favipiravir was calculated by evaluating the chromatographic data obtained in the investigated hydro‐organic and micellar solvent‐free mixtures using two different calculation methods. In the determination of their pKa values in water from the pKa values of the investigated compounds determined in hydro‐organic mixtures, the relationship between some macroscopic values of acetonitrile, methanol, ethanol and the values was used, while the values of the compounds could be determined directly in micellar solvent‐free mobile phases. It was found that the directly and indirectly calculated values were quite compatible with each other. Using the value calculated for favipiravir, the ionization percentages at different pH values (1–12) were calculated by the Henderson‐Hasselbalch equation. The environmental impact of the RPLC methods used in this study was assessed using the Green Metrics tools GAPI and AGREE.

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Development of a colorimetric assay for quantification of favipiravir in human serum using ferrihydrite

Cited by 8 publications

References 34 publications

Novel colorimetric approach for amikacin estimation in pure powder and its pharmaceutical formulations

Novel colorimetric approach for amikacin estimation in pure powder and its pharmaceutical formulations

Development and Evaluation of Potato Starch and Chitosan Modified Capecitabine Nanoparticles for Enhanced Colon Cancer Treatment: A Comprehensive Study on PhysicalProperties, In vitro Efficacy, and In vivo Targeting

Switch from The Traditional RPLC Method to the Green RPLC Methods in The Determination of The Ionization Constant (pK_a) of Favipiravir

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Development of a colorimetric assay for quantification of favipiravir in human serum using ferrihydrite

Cited by 8 publications

References 34 publications

Novel colorimetric approach for amikacin estimation in pure powder and its pharmaceutical formulations

Novel colorimetric approach for amikacin estimation in pure powder and its pharmaceutical formulations

Development and Evaluation of Potato Starch and Chitosan Modified Capecitabine Nanoparticles for Enhanced Colon Cancer Treatment: A Comprehensive Study on PhysicalProperties, In vitro Efficacy, and In vivo Targeting

Switch from The Traditional RPLC Method to the Green RPLC Methods in The Determination of The Ionization Constant (pKa) of Favipiravir

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Product

Resources

About

Switch from The Traditional RPLC Method to the Green RPLC Methods in The Determination of The Ionization Constant (pK_a) of Favipiravir