“…MNBs were cryopreserved via vitrification as previously reported [ 9 , 10 , 12 ]. First, the MNBs were equilibrated in base medium (MEM α supplemented with 20% fetal bovine serum (FBS; SH30070.03, HyClone Laboratories, Inc., Logan, UT, USA) and 1% antibiotic–antimycotic solution [15,240,062; Thermo Fisher Scientific, Waltham, MA, USA]) with 7.5% ethylene glycol (EG; 055-00996; Wako, Osaka, Japan) and 7.5% dimethyl sulfoxide (DMSO; 317275-100ML; Millipore, Burlington, MA, USA) on ice for 15 min and soaked in base medium with 15% EG and 15% DMSO on ice for an additional 15 min.…”