Development of a Cyclone-Based Aerosol Sampler with Recirculating Liquid Film: Theory and Experiment

Sigaev, Gennady I.; Tolchinsky, Alexander D.; Sigaev, Vladimir I.; Soloviev, Konstantin G.; Varfolomeev, A. N.; Chen, Bean T.

doi:10.1080/02786820600596891

Cited by 22 publications

(13 citation statements)

References 6 publications

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“…Its operating principle is similar to the system developed by Sigaev et al (2006). The commercially available BWWC is designed to sample air at a flow rate of 400 l/min and concentrate the particles into a liquid volume of 12 ml.…”

Section: Introductionmentioning

confidence: 99%

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Collection characteristics of a batch-type wetted wall bioaerosol sampling cyclone

et al. 2009

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The collection efficiency and sample retention of a batch-type wetted wall bioaerosol sampling cyclone (BWWC) were experimentally characterized. The BWWC is designed to sample air at 400 l/min and concentrate the particles into 12 ml of water. Aerosol is transported into a cylindrically-shaped axial flow cyclone through a tangential slot and the particles are impacted on the inner wall, which is wetted by air shear acting on a liquid pool at the base of the cyclone. The retention of collected particles and the aerosol collection efficiency of the BWWC were evaluated with polystyrene latex beads (PSL), sodium fluorescein/oleic acid droplets, and Bacillus atrophaeus (aka BG) spores. The retention of particles was determined by adding hydrosol directly into the device, running the BWWC for a pre-set period of time, and then determining the amount of particulate matter recovered relative to the initial amount. For 1-lm diameter PSL, 90% of the particles were recoverable from the cyclone body immediately after their introduction; however, only 10% were retained in the collection liquid after 8 h of operation. The aerosol sampling efficiency was determined by comparing the amount of particulate matter collected in the liquid with that collected by a reference filter. The collection efficiency was 50-60% for 1-and 3-lm polystyrene (PSL) particles, and 1.5% for 10-lm oleic acid particles. The efficiency for 3-lm oleic acid droplets was 35%. Explanations are provided for the difference between liquid and solid particle behavior, and for the low efficiency for the large liquid particles. The collection efficiency for single spore BG was slightly lower than that for 1-lm PSL.

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Section: Introductionmentioning

confidence: 99%

“…A batch-type wetted wall cyclone (BWWC), which samples at a flow rate of 60 l/min and concentrates the particulate matter into 10 ml of liquid, was developed by Sigaev et al (2006). Aerosol is accelerated as it enters the cyclone body, and larger particles are impacted on the inner wall, which is continuously wetted by liquid.…”

Section: Introductionmentioning

confidence: 99%

Collection characteristics of a batch-type wetted wall bioaerosol sampling cyclone

et al. 2009

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“…16 The PAS-5 sampler is an improved version of its predecessor. 13,17 As shown in Fig. 1b, the PAS-5 sampler has a column-like body that is 9.6 cm in height by 3.8 cm in width.…”

Section: Test Samplersmentioning

confidence: 99%

Evaluation of physical sampling efficiency for cyclone-based personal bioaerosol samplers in moving air environments

Tolchinsky²,

Chen

et al. 2012

J. Environ. Monit.

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The need to determine occupational exposure to bioaerosols has notably increased in the past decade, especially for microbiology-related workplaces and laboratories. Recently, two new cyclone-based personal bioaerosol samplers were developed by the National Institute for Occupational Safety and Health (NIOSH) in the USA and the Research Center for Toxicology and Hygienic Regulation of Biopreparations (RCT & HRB) in Russia to monitor bioaerosol exposure in the workplace. Here, a series of wind tunnel experiments were carried out to evaluate the physical sampling performance of these two samplers in moving air conditions, which could provide information for personal biological monitoring in a moving air environment. The experiments were conducted in a small wind tunnel facility using three wind speeds (0.5, 1.0 and 2.0 m s −1 ) and three sampling orientations (0°, 90°, and 180°) with respect to the wind direction. Monodispersed particles ranging from 0.5 to 10 μm were employed as the test aerosols. The evaluation of the physical sampling performance was focused on the aspiration efficiency and capture efficiency of the two samplers. The test results showed that the orientation-averaged aspiration efficiencies of the two samplers closely agreed with the American Conference of Governmental Industrial Hygienists (ACGIH) inhalable convention within the particle sizes used in the evaluation tests, and the effect of the wind speed on the aspiration efficiency was found negligible. The capture efficiencies of these two samplers ranged from 70% to 80%. These data offer important information on the insight into the physical sampling characteristics of the two test samplers.

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“…A comparison of the two methods indicated that collection e$ciencies for filtration systems are generally greater than that of impingement devices, especially with respect to particles ῍0.2 mm 1), 16), 25) . Filtrations systems with appropriate filters have been shown to remove ῍98῎ of ῍0.3 mm particles and collect 74῎ of airborne Gram negative bacteria (1), whereas impingement technology collected 50ῌ90῎ of ῍0.3 mm particles 1), 9) and demonstrate a similar decrease in the number of Gram negative bacteria collected.…”

Section: Aerosol Samplingmentioning

confidence: 99%

“…Filtrations systems with appropriate filters have been shown to remove ῍98῎ of ῍0.3 mm particles and collect 74῎ of airborne Gram negative bacteria (1), whereas impingement technology collected 50ῌ90῎ of ῍0.3 mm particles 1), 9) and demonstrate a similar decrease in the number of Gram negative bacteria collected. Additionally, filtration systems were demonstrated to be capable of collecting significantly larger volume samples (up to 2 orders of magnitude) than impingement technology 16), 21), 25) . Given the presumed low biomass of spacecraft assembly facilities, the volume of air which can be sampled and the e$ciency of sampling make filtration technology the best currently available option for the microbial detection in spacecraft assembly facilities and other clean-rooms.…”

Section: Aerosol Samplingmentioning

confidence: 99%

Overview of Methodologies to Sample and Assess Microbial Burden in Low Biomass Environments

Bruckner

Venkateswaran

2007

Nihon Shokuhin Biseibutsu Gakkai zasshi

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The detection and evaluation of microbial contamination is the goal of many programs in the medical, food, pharmaceutical, and environmental monitoring/biothreat detection industries. Similarly, NASA's Planetary Protection program strives to assess, as well as prevent, microbial contamination in spacecraft assembly facilities and on spacecraft themselves. The development of improved methods and technologies designed to facilitate these common goals is therefore important. This review will focus on instrumentation and techniques currently being utilized or evaluated for use by the Planetary Protection program in the clean room environments of spacecraft assembly facilities. Current sampling technologies (novel sampling instrumentation and di#erent collection materials) were compared with NASA standard protocols under field and controlled conditions. A brief literature review was presented to augment the comparisons. Additionally, as the integrity of sample analyses is directly dependent on both the e$ciency of sample collection and the quality of sample processing, a novel nucleic acid extraction instrument was also evaluated. The impact of these new technologies will be discussed with respect to sample collection, processing, and analyses from the low biomass environments present in NASA clean rooms, although the findings may be applied in a broader context to other industries and programs as well as to the development of future integrated biosensors.

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Development of a Cyclone-Based Aerosol Sampler with Recirculating Liquid Film: Theory and Experiment

Cited by 22 publications

References 6 publications

Collection characteristics of a batch-type wetted wall bioaerosol sampling cyclone

Collection characteristics of a batch-type wetted wall bioaerosol sampling cyclone

Evaluation of physical sampling efficiency for cyclone-based personal bioaerosol samplers in moving air environments

Overview of Methodologies to Sample and Assess Microbial Burden in Low Biomass Environments

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