Development of a Detector for Liquid Chromatography Based on Aerosol Chemiluminescence on Porous Alumina

Lv, Yi; Zhang, Sichun; Liu, Guo-Hong; Huang, Minwen; Zhang, Xinrong

doi:10.1021/ac048816w

Cited by 51 publications

(44 citation statements)

References 30 publications

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“…A novel aerosol CTL detector based on porous alumina was developed according to the generated CTL emission from catalytic oxidation of the analytes [30]. This aerosol CTL-based detector has three main processes: nebulization of solution; CTL emission on surface of porous alumina material; and optical detection.…”

Section: Recognition Of Organic Compounds In Aqueous Solutions By Chementioning

confidence: 99%

Assembly of Nanostructures for Taste Sensing

Zhang

Wang

2011

NanoBiosensing

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Section: Recognition Of Organic Compounds In Aqueous Solutions By Chementioning

confidence: 99%

Assembly of Nanostructures for Taste Sensing

Zhang

Wang

2011

NanoBiosensing

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“…Poly(ethylene glycol) or its copolymers have been analyzed by various analytical methods involving LC/MS, 15 sizeexclusion chromatography hyphenated with pyrolysis-GC, 16 two-dimensional LC, 17 reversed-phase LC, 18 LC with aerosol chemiluminescence-based detection, 19 etc. Most of these methods are qualitative and sensitive.…”

Section: Retention Behavior Of Ethylene Glycol Oligomersmentioning

confidence: 99%

Indirect Detection of Ethylene Glycol Oligomers Using a Contactless Conductivity Detector in Capillary Liquid Chromatography

2009

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Ethylene glycol oligomers were visualized by indirect conductimetric detection based on dilution of the mobile phase due to the analytes. A high electrical conductivity background was maintained by the addition of 5 mM sodium nitrate in the mobile phase, and the analytes were visualized by decreases in the background when they eluted. A capacitively coupled contactless conductivity detector was convenient to monitor effluents from the microcolumn with minimum extra-column band broadening. The signals as negative peaks were linear to the concentration of the analytes, and a concentration detection limit of 0.025% was achieved for tetraethylene glycol at S/N=3, corresponding to the mass detection limit of 38 ng for 0.15 μl injection. The logarithm of the retention factor of ethylene glycol oligomers was linear to the degree of polymerization (DP) as well as to the acetonitrile composition in the mobile phase. These situations allowed us to estimate the DP of eluted ethylene glycol oligomers by using a few oligomers with known DP. The dynamic reserve, defined as the ratio of the background to its noise level achieved under the present conditions, was 2.3 × 10 5 which was much larger than that achieved by UV absorption detection. The present method was applied to profile ethylene glycol oligomers contained in commercially available PEG reagents.

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“…The ELSD is suitable to detect nonvolatile compounds such as lipids, carbohydrates, and pharmaceutical compounds, which show weak optical absorbance in the UV-Visible region. However, the ELSD is unable to be applied to the measurement of volatile analytes [25].…”

Section: Hplc Detectorsmentioning

confidence: 99%

Suspended Microchannel Resonators for Ultralow Volume Universal Detection

et al. 2008

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Universal detectors that maintain high sensitivity as the detection volume is reduced to the sub-nanoliter scale can enhance the utility of miniaturized total analysis systems (ji-TAS). Here the unique scaling properties of the suspended microchannel resonator (SMR) are exploited to show universal detection in a 10 pL analysis volume with a density detection limit of -1 [gg/cm 3 (10 Hz bandwidth) and a linear dynamic range of six decades. Analytes with low UV extinction coefficients such as polyethylene glycol (PEG) 8 KDa, glucose, and glycine are measured with molar detection limits of 0.66 RM, 13.5 gM, and 31.6 JIM, respectively. To demonstrate the potential for real-time monitoring, gel filtration chromatography was used to separate different molecular weights of PEG as the SMR acquired a chromatogram by measuring the eluate density. This work suggests that the SMR could offer a simple and sensitive universal detector for various separation systems from liquid chromatography to capillary electrophoresis. Moreover, since the SMR is itself a microfluidic channel, it can be directly integrated into g-TAS without compromising overall performance. AcknowledgementsFirst of all, I wish to thank Professor Scott Manalis for the opportunity to do my thesis research in his laboratory. Scott has given me invaluable guidance that fosters creativity, teamwork, enthusiasm, and handson research. I also want to thank all group members who contributed to this work: Thomas Burg originally developed the SMR and related setups which I used for my research inline with HPLC system. He also gave me countless advices regarding experiment. Will Grover was an indispensable resource for everything from the knowledge involving HPLC to experimental details. Michel Godin was a good consultant who gave me an insight to research in general. Scott Knudsen helped me dealing with problems involving chemistry and biology with great passion. Yao-Chung (Wesley) Weng gave me a great help in dealing with electronics such as oscillating circuits. Furthermore, the passion of Rumi Chunara, diligence of Andrea Bryan, optimism of Marcio von Muhlen, and discretion of Phillp Dextras has been constant sources of motivation for me. I also thank Ken Babcock and Alison Skelley for valuable discussions.

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Development of a Detector for Liquid Chromatography Based on Aerosol Chemiluminescence on Porous Alumina

Cited by 51 publications

References 30 publications

Assembly of Nanostructures for Taste Sensing

Assembly of Nanostructures for Taste Sensing

Indirect Detection of Ethylene Glycol Oligomers Using a Contactless Conductivity Detector in Capillary Liquid Chromatography

Suspended Microchannel Resonators for Ultralow Volume Universal Detection

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