Development of a Diagnostic DNA Probe for the Fruit Flies &lt;I&gt;Ceratitis capitata&lt;/I&gt; and &lt;I&gt;Ceratitis rosa&lt;/I&gt; (Diptera: Tephritidae) Using Amplified Fragment-Length Polymorphism

Kakouli‐Duarte, Thomais; Casey, Dáire; Burnell, Ann M.

doi:10.1603/0022-0493-94.4.989

Cited by 18 publications

(11 citation statements)

References 36 publications

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“…For this method, a repetitive DNA sequence was isolated from the genome of C. capitata and then used as a probe. It identified C. capitata and C. rosa fast and reliably among a collection of other fruit fly species and other insects (Kakouli-Duarte et al, 2001).…”

Section: Laboratory Testing and Pest Identificationmentioning

confidence: 97%

Pest survey card on Ceratitis rosa and Ceratitis quilicii

Schrader¹,

Camilleri²,

Diakaki³

et al. 2019

EFS3

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This pest survey card was prepared in the context of the mandate on plant pest surveillance (EFSA-Q-2017-00831), upon request by the European Commission. The purpose of this document is to assist the Member States in planning annual survey activities of quarantine organisms using a statistically sound and risk-based pest survey approach, in line with the current international standards. The data requirements for such activity include the pest distribution, its host range, its biology, risk factors as well as available detection and identification methods. This document is part of a toolkit that consists of pest-specific documents, such as the pest survey cards and generic documents relevant for all pests to be surveyed, including, the general survey guidelines and statistical software such as RiBESS+.

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Section: Laboratory Testing and Pest Identificationmentioning

confidence: 97%

Pest survey card on Ceratitis rosa and Ceratitis quilicii

Schrader¹,

Camilleri²,

Diakaki³

et al. 2019

EFS3

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“…Markers have been derived from mitochondrial and nuclear genes such as cytochrome oxidase I (COI) 6 , the ribosomal internal transcribed spacer region 2 (ITS 2) 7 , and 28S rDNA 8 . Also diverse methods such as amplified fragment length polymorphisms 9 , single-stranded conformational polymorphisms 10 , and simple sequence repeats or microsatellites have been employed for these analyses 11,12 . Among the markers and methods used, microsatellites have a number of advantages for population genetic analysis in that they tend to be highly polymorphic and exhibit co-dominant inheritance.…”

Section: Introductionmentioning

confidence: 99%

Microsatellite marker variation in populations of the melon fly parasitoid, Psyttalia fletcheri

Homchan¹,

Haymer²,

Kitthawee³

2014

ScienceAsia

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The parasitoid Psyttalia fletcheri (Silvestri) is an important natural enemy of the melon fly, Bactrocera cucurbitae (Coquillett). Melon fly infestations are responsible for extensive losses of cucurbit production worldwide, and P. fletcheri has been used for some time in biological control programmes attempting to deal with this pest. However, there is a general lack of knowledge of the genetic structure of populations of P. fletcheri, and the development of this information is key to the effective use of this parasitoid. In this study, we isolated several novel microsatellite loci to investigate the genetic structure of P. fletcheri populations from six locations in Thailand. All the loci analysed here were polymorphic, and the mean number of alleles per locus ranged from 4.2-8.6. Heterozygote deficiencies were noticed in most populations. Overall FST estimates showed moderate genetic differentiation among P. fletcheri populations with a jackknife mean of 0.084. However, pairwise FST calculations revealed that 11 out of 15 population comparisons showed genetic differentiation. The greatest level of differentiation was also found for the population that had the lowest value for genetic diversity. In contrast, populations with high levels of genetic variation did not show significant genetic differentiation, nor did they show significant isolation by distance. An unrooted dendrogram constructed from Nei's genetic distance values also confirmed that one population from the south of Thailand can be separated from the others.

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“…Although the AFLP technique is widely used in plant research, its use in insects is so far limited. Recently the AFLP technique has been used to understand genetic differentiation of insects (Kakouli-Duarte et al, 2001;Salvato et al, 2002). The study presented here concerns two aims by AFLP analysis: (i) to identify the two sibling species; (ii) to evaluate the interspecific genetic divergence and intraspecific genetic variation.…”

Section: Introductionmentioning

confidence: 99%

Genetic differentiation of Helicoverpa armigera (Hübner) and H. assulta (Guenée) (Lepidoptera: Noctuidae) based on AFLP markers

Ming

Wang

2006

Insect Science

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Here we use amplified fragment length polymorphism (AFLP) to assess genetic differentiation of Helicoverpa armigera and H. assulta. The results indicated that both species‐specific fingerprints and cluster analysis showed the ability of AFLP technique to discriminate the two sibling species; among a total 1963 AFLP markers amplified from nine primer combinations: 777 (39.6%) were H. armigera‐specific, 602 (30.7%) were H. assulta‐specific, and 584 (29.7%) were common bands. The mean number of H. armigera‐specific bands was significantly more than that of H. assulta‐specific bands for nine primer combinations (P < 0.05); the intraspecific distance of H. armigera and H. assulta was 0.123 0 and 0.110 7 respectively, and the interspecific distance was 0.178 3. In addition, the percentage of polymorphic loci and estimated average heterozygosity were used to estimate genetic diversity of the two species. This study therefore demonstrates that AFLP analysis is a sensitive and reliable technique to study genetic differentiation and genetic relationships between species and provides sufficient molecular markers for future linkage map construction, location and eventual cloning of genes involved in traits differentiation.

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Development of a Diagnostic DNA Probe for the Fruit Flies <I>Ceratitis capitata</I> and <I>Ceratitis rosa</I> (Diptera: Tephritidae) Using Amplified Fragment-Length Polymorphism

Cited by 18 publications

References 36 publications

Pest survey card on Ceratitis rosa and Ceratitis quilicii

Pest survey card on Ceratitis rosa and Ceratitis quilicii

Microsatellite marker variation in populations of the melon fly parasitoid, Psyttalia fletcheri

Genetic differentiation of Helicoverpa armigera (Hübner) and H. assulta (Guenée) (Lepidoptera: Noctuidae) based on AFLP markers

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