Development of a diploid cell line from fetal rhesus monkey lung for virus vaccine production

Wallace, Roslyn E.; Vasington, Paul J.; Petricciani, John C.; Hopps, Hope E.; Lorenz, Douglas E.; Kadaňka, Zdeněk

doi:10.1007/bf02619056

Cited by 36 publications

(11 citation statements)

References 26 publications

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“…Reassortment with a human rotavirus VP7 serotype 3 strain was not necessary because RRV belongs to this serotype. Each of these strains was plaque purified three times in AGMK cells, and vaccine lots were subsequently prepared in DBS-FRhL 2 cells, a semicontinuous diploid cell strain developed as a suitable vaccine substrate by the predecessor of the Office of Biologics of the U.S. Food and Drug Administration (144).…”

Section: Human-rhesus Rotavirus Reassortant Vaccinesmentioning

confidence: 99%

Rotavirus vaccines: an overview

1996

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Rotavirus vaccine development has focused on the delivery of live attenuated rotavirus strains by the oral route. The initial "Jennerian" approach involving bovine (RIT4237, WC3) or rhesus (RRV) rotavirus vaccine candidates showed that these vaccines were safe, well tolerated, and immunogenic but induced highly variable rates of protection against rotavirus diarrhea. The goal of a rotavirus vaccine is to prevent severe illness that can lead to dehydration in infants and young children in both developed and developing countries. These studies led to the concept that a multivalent vaccine that represented each of the four epidemiologically important VP7 serotypes might be necessary to induce protection in young infants, the target population for vaccination. Human-animal rotavirus reassortants whose gene encoding VP7 was derived from their human rotavirus parent but whose remaining genes were derived from the animal rotavirus parent were developed as vaccine candidates. The greatest experience with a multivalent vaccine to date has been gained with the quadrivalent preparation containing RRV (VP7 serotype 3) and human-RRV reassortants of VP7 serotype 1, 2, and 4 specificity. Preliminary efficacy trial results in the United States have been promising, whereas a study in Peru has shown only limited protection. Human-bovine reassortant vaccines, including a candidate that contains the VP4 gene of a human rotavirus (VP4 serotype 1A), are also being studied.

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Section: Human-rhesus Rotavirus Reassortant Vaccinesmentioning

confidence: 99%

Rotavirus vaccines: an overview

1996

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“…3T3 mouse fibroblastic cells, clone D152, from BALB/e mice [8] were obtMned from g. Bassin (NCI, Bethesda, MD), The fetal rhesus lung cell line,'DBS-FRHL [21] was a kind gift of G. Todaro (NCI, Bethesda, ND), and the epithelial AV3 human cell line [4] was purchased from American Type Cellular Collection (Rockville, MD). The three cell lines were propagated in Me Coy 5A medium suplemented with 10% fetal calf serum…”

Section: Cellsmentioning

confidence: 99%

Studies on in vitro Interferon induction capacity and Interferon sensitivity of Simian Foamy viruses

Rhodes-Feuillette

Saal

Lasneret

et al. 1987

Archives of Virology

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We demonstrate that Simian Foamy viruses (SFV) types 1, 2, 4 and 10 do not induce Interferon (IFN) production in mouse and primate (simian and human) cell lines, but that their cytopathogenic effect is blocked by this viral inhibitor. The mechanisms of action of IFN seems to be different from that of other Retroviridae. No trapping of virions appears in treated cells examined by ectron microscopy. Moreover, neither precursor nor mature virus particles were observed in infected cultures submitted to IFN treatment.

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“…Final passage in fetal rhesus lung (FRhL) cells is frequently used for virus seed and vaccine lot production (8). The FRhL cell strain is a normal diploid cell suitable for the production of vaccines for human use (55). These cells have been shown to support the replication of all four DENV serotypes to high titers (9,31).…”

mentioning

confidence: 99%

Passage of Dengue Virus Type 4 Vaccine Candidates in Fetal Rhesus Lung Cells Selects Heparin-Sensitive Variants That Result in Loss of Infectivity and Immunogenicity in Rhesus Macaques

Áñez

Men

Eckels

et al. 2009

J Virol

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Dengue virus (DENV) infects humans via the bite of infected mosquitoes, principally Aedes aegypti. DENV infections can be asymptomatic or cause a spectrum of illnesses that range from mild dengue fever to a severe, life-threatening disease characterized by dengue hemorrhagic fever/dengue shock syndrome (13, 38). The four DENV serotypes (DENV type 1 [DENV-1] to DENV-4) are the most important members of the genus Flavivirus in terms of morbidity, geographic distribution, and socioeconomic burden (1, 12). Several other members of the flaviviruses, including yellow fever virus (YFV), Japanese encephalitis virus (JEV), West Nile virus, and tickborne encephalitis virus, are also important human pathogens.The flavivirus virion is a spherical enveloped particle with icosahedral symmetry. It has a relatively simple structure, consisting of an inner nucleocapsid-virus RNA core and an outer lipid bilayer membrane into which a small ϳ9-kDa membrane protein (M) and a larger ϳ54-kDa envelope protein (E) are embedded. The E protein, which is approximately 500 amino acids in length, is the major antigen responsible for attachment to the cell surface, viral entry mediated by endocytosis, fusion with endosomal membranes, and the eliciting of host immune responses. There are 180 copies of E in the form of homodimers arranged in a tight array on the smooth virion surface without major spikes (21,37,48). Structural analysis indicates that each E monomer is folded into three structurally distinct domains, termed domains I, II, and III (DI, DII, and DIII, respectively). DIII has an immunoglobulin-like fold, a structural feature shared by many cell-adhesive molecules and receptor-binding proteins. DIII has been proposed to be responsible for binding interaction with cell surface receptors (16,48). A number of mosquito-borne flavivirus E proteins contain a sequence motif in DIII that is recognized by integrin receptors. Mutations affecting cell attachment that cluster in this region are associated with attenuation of virulence and cell tropism (26,27,29,43,53).A specific cell surface receptor has not been clearly identified for DENV or any other flavivirus. Studies focusing on the mechanisms of viral binding and entry in mosquito C6/36 cells (42, 56) or mammalian cells (35,41) have suggested a number of proteins of various sizes that are capable of binding the DENV virion. Recently, the C-type lectin DC-SIGN was found to be capable of facilitating DENV infection of dendritic cells (51,52). It has been proposed that flaviviruses could also utilize other less specific molecules on the cell surface as coreceptors for initial adsorption and infection. Infection of DENV-2 was first found to depend on heparan sulfate (HS), a major constituent of the extracellular matrix and a surface component of most mammalian cells, for binding interaction and infectivity of cultured cells (6). In that study the authors identified sequences of two HS binding sites in E, one in DIII and the other in the junction between DI and DIII. Although HS is essential ...

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Development of a diploid cell line from fetal rhesus monkey lung for virus vaccine production

Cited by 36 publications

References 26 publications

Rotavirus vaccines: an overview

Rotavirus vaccines: an overview

Studies on in vitro Interferon induction capacity and Interferon sensitivity of Simian Foamy viruses

Passage of Dengue Virus Type 4 Vaccine Candidates in Fetal Rhesus Lung Cells Selects Heparin-Sensitive Variants That Result in Loss of Infectivity and Immunogenicity in Rhesus Macaques

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