Development of a disperse dye immunochromatographic test for the detection of antibodies against infectious bursal disease virus

Wang, Shwu-Jinng; Chang, Wei-Feng; Wang, Min-Ying; Hsiung, Kuang-Pin; Liu, Yung-Chuan

doi:10.1016/j.vetimm.2008.05.022

Cited by 6 publications

(1 citation statement)

References 24 publications

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“…On the other hand, at a higher dye concentration the epitopes of the antibody might be shielded by dye molecules which cause antibody inactivation. Also, in the higher dye concentrations, the aggregation of dyed-antibody may occur, which could result in background of NC membrane during the assay process [28]. It is also observed that higher color intensities were obtained when dyeing was conducted with a longer incubation time.…”

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confidence: 99%

Development of a Disperse Dye Immunoassay Technique for Detection of Antibodies against Neospora caninum in Cattle

et al. 2013

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In this study a disperse dye immunoassay method was standardized and evaluated for detection of antibodies against Neospora caninum in cattle. Sera from 150 cattle with a recent history of abortion were collected and tested by commercial ELISA kit and a standardized in-house dye immunoassay system. The positivity rate for the sera used in this study was 34.6% for the disperse dye immunoassay (DDIA) compared to 32% obtained by ELISA kit. This study showed no significant difference between DDIA and ELISA. The results indicated that the DDIA provide an economic, simple, rapid and robust test for detection of N. caninum infection in cattle.

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confidence: 99%