Development of a disposable and easy-to-fabricate microfluidic PCR device for DNA amplification

Mashouf, H.; Talebjedi, Bahram; Tasnim, Nishat; Tan, Maia; Alousi, Sahar; Pakpour, Sepideh; Hoorfar, Mina

doi:10.1016/j.cep.2023.109394

Cited by 8 publications

(1 citation statement)

References 51 publications

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“…Usually, these small devices require the production of micro and nano-scale patterns with different shapes and geometry. For instance, microfluidic devices with micro to nanopattern structures had been used to maximize the sensitivity in detecting analytes [7][8][9]. On the other hands, micro lenses are utilized to enhance camera image quality [10] and light guide plate (LGP) also contain microstructure pattern on the surface of the material to distribute light evenly [11,12].…”

Section: Introductionmentioning

confidence: 99%

Fabrication of Polymer Light Guide Plate (LGP) using Direct CO2 Laser Structuring

Norfazilasari Yasman,

Raja Murfiqah Raja Mohamad Fouzy,

Mohd Zairulnizam Mohd Zawawi

2024

ARAM

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Light guide plates (LGPs) are an integral component that help distribute illumination from the light source in a variety of applications. Therefore, the design and quality of the microstructure pattern in LGPs play a significant role in achieving high luminous efficiency and light uniformity. This study investigates the relationship between laser power and laser scanning speed to the formation of micro-dots by using CO2 direct laser structuring on PMMA. Furthermore, the effect of different micro-dots pitch to the luminance was also evaluated using the luminance meter. Our finding shows that increase of laser power and decrease of laser scanning speed resulted in larger micro-dots diameter and deeper micro-dots. The results also showed that the smaller the pitch, the higher the luminance reading. Overall, the low-cost CO2 direct laser structuring demonstrated in the study able to produce consistent micro-dots pattern diameter and height which is suitable for fabrication of LGPs in the mass production.

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Section: Introductionmentioning

confidence: 99%

Fabrication of Polymer Light Guide Plate (LGP) using Direct CO2 Laser Structuring

Norfazilasari Yasman,

Raja Murfiqah Raja Mohamad Fouzy,

Mohd Zairulnizam Mohd Zawawi

2024

ARAM

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An Integrated Micro‐Heating System for On‐Chip Isothermal Amplification of African Swine Fever Virus Genes

Sun,

Shi,

Tan

et al. 2024

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The loop‐mediated isothermal amplification (LAMP) is widely used in the laboratory to facilitate rapid DNA or RNA detection with a streamlined operational process, whose properties are greatly dependent on the uniformity and rise rate of temperature in the reaction chambers and the design of the primers. This paper introduces a planar micro‐heater equipped with an embedded micro‐temperature sensor to realize temperature tunability at a low energy cost. Moreover, a control system, based on the Wheatstone bridge and proportional, integral, and derivative (PID) control, is designed to measure and adjust the temperature of the micro‐heater. The maximum temperature rise rate of the designed micro‐heater is ≈8 °C s−1, and it only takes ≈60 s to reach the target temperature. Furthermore, a designed plasmid, containing the B646L gene of African Swine Fever Virus (ASFV), and a set of specific primers, are used to combine with the designed micro‐heating system to implement the LAMP reaction. Finally, the lateral flow assay is used to interpret the amplification results visually. This method can achieve highly sensitive and efficient detection of ASFV within 40 min. The sensitivity of this on‐chip gene detection method is 8.4 copies per reaction, holding great potential for applications in DNA and RNA amplification.

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A role of integrated microheaters in a microfluidics based point-of-care-testing and beyond for healthcare applications

Kulkarni,

Yashas,

Vyas

2024

Applied Materials Today

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Development of a disposable and easy-to-fabricate microfluidic PCR device for DNA amplification

Cited by 8 publications

References 51 publications

Fabrication of Polymer Light Guide Plate (LGP) using Direct CO2 Laser Structuring

Fabrication of Polymer Light Guide Plate (LGP) using Direct CO2 Laser Structuring

An Integrated Micro‐Heating System for On‐Chip Isothermal Amplification of African Swine Fever Virus Genes

A role of integrated microheaters in a microfluidics based point-of-care-testing and beyond for healthcare applications

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