2002
DOI: 10.1046/j.1537-2995.2002.00248.x
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Development of a DNA‐based genotyping method for the Diego blood group system

Abstract: A simple, accurate, and inexpensive DNA-based PCR-SSP method was established for Di genotyping. The typing results can be visualized on a single photograph within 3 hours, making this reliable method suitable for large-scale typing of potential blood donors without serologic backup.

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Cited by 16 publications
(27 citation statements)
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“…The genotype distributions of DI loci were consistent with Hardy–Weinberg equilibrium ( P > 0·05). Moreover, the frequencies of the DI1 and DI2 alleles in the Chinese Han population were 0·0247 and 0·9753, respectively (Table 2), which were similar to that reported previously [10]. Partial DNA sequencing chromatograms from these samples were shown in Fig.…”
Section: Resultssupporting
confidence: 87%
See 1 more Smart Citation
“…The genotype distributions of DI loci were consistent with Hardy–Weinberg equilibrium ( P > 0·05). Moreover, the frequencies of the DI1 and DI2 alleles in the Chinese Han population were 0·0247 and 0·9753, respectively (Table 2), which were similar to that reported previously [10]. Partial DNA sequencing chromatograms from these samples were shown in Fig.…”
Section: Resultssupporting
confidence: 87%
“…The alleles of DI1 and DI2 are based on an SNP in the DNA sequence. Although a simple and rapid PCR‐ASP genotyping method for Diego antigens had been established [10], the identification of new variants could not be determined by this method. In view of that it is essential to establish a reliable and accurate genotyping method for Diego blood group antigens.…”
Section: Discussionmentioning
confidence: 99%
“…In contrast to the Caucasians, the antigens of the Lutheran (Lu a ), Kell (K), Colton (Co b ), LW (LW b ) and Knops (Kn b ) blood group systems are not found in the Chinese population. In contrast, Di a antigen of Diego blood group and Mur and St a antigens of MNS blood group are found with a higher frequency of 4·54–7% , 0·5–24·7% and 2·6% (26/1004, Z Wang, YN Liang, JZ Wen, L Wei, GP Luo, YL Ji our unpublished data), respectively, in the population from the mainland of China. These distribution studies indicate that antigen positive individuals following transfusion are different in the Chinese population compared with the Caucasians, which could produce different clinically significant alloantibodies.…”
Section: The Distribution Study Of Blood Group Systems In the Chinesementioning
confidence: 83%
“…The Di a and Di b allele were determined with PCR SSP method according to Wu and colleagues 21 . Jk a and Jk b genotyping was performed with the commercially available test KKD‐SSP (Innotrain Diagnostik, Kronberg, Germany) according to the manufacturer's instructions.…”
Section: Methodsmentioning
confidence: 99%