Development of a DNA microarray-based multiplex assay of avian influenza virus subtypes H5, H7, H9, N1, and N2

Shi, Lisong; Sun, Jui Sheng; Zp, Yang; Hm, Bao; Jiang, Yongping; Yz, Xiong; Cao, Deliang; Xw, Yu; Hl, Chen; Sm, Zheng; Xr, Wang

doi:10.4149/av_2014_01_14

Cited by 5 publications

(3 citation statements)

References 14 publications

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“…These assays typically rely on a single amplified target as an indicator of the presence of a pathogen. While numerous microarray-based assays utilizing multiple probes per target for the detection of human and animal-origin influenza have appeared (Mukherjee and Chakrabarti, 2012;Heydarov et al, 2017;Paulin et al, 2014;Shi et al, 2014;Ryabinin et al, 2011;Gall et al, 2009;Li et al, 2009), none are broadly commercially available. Next generation sequencing of influenza viruses provides the ability for complete characterization and is now routine in the three National Influenza Surveillance Reference Centers and at the CDC.…”

Section: Introductionmentioning

confidence: 99%

Analytical evaluation of the microarray-based FluChip-8G Influenza A+B Assay

Taylor

Dawson

Blair

et al. 2019

Journal of Virological Methods

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A B S T R A C TBackground: Influenza causes a significant annual disease burden, with characterization of the infecting virus important in clinical and public health settings. Rapid immunoassays are fast but insensitive, whereas real-time RT-PCR is sensitive but susceptible to genetic mutations and often requires multiple serial assays. The FluChip-8G Influenza A+B Assay provides type and subtype/lineage identification of influenza A and B, including nonseasonal A viruses, in a single microarray-based assay with same day turnaround time. Objective: To evaluate key analytical performance characteristics of the FluChip-8G Influenza A+B Assay. Study design: Analytical sensitivity, cross-reactivity, and multi-site reproducibility were evaluated. Results: The limit of detection (LOD) for the FluChip-8G influenza A+B Assay ranged from 5.8 × 10 2 -1.5 × 10 5 genome copies/mL, with most samples ∼2 × 10 3 genome copies/mL (∼160 genome copies/reaction). Fifty two (52) additional strains were correctly identified near the LOD, demonstrating robust reactivity. Two variant viruses (H1N1v and H3N2v) resulted in dual identification as both "non-seasonal influenza A" and A/ H1N1pdm09. No reproducible cross-reactivity was observed for the 34 organisms tested, however, challenges with internal control inhibition due to crude growth matrix were observed. Lastly, samples tested near the LOD showed high reproducibility (97.0% (95% CI 94.7-98.7)) regardless of operator, site, reagent lot, or testing day. Conclusion: The FluChip-8G Influenza A+B Assay is an effective new method for detecting and identifying both seasonal and non-seasonal influenza viruses, as revealed by good sensitivity and robust reactivity to 52 unique strains of influenza virus. In addition, the lack of cross-reactivity to non-influenza pathogens and high lab-to-lab reproducibility highlight the analytical performance of the assay as an alternative to real-time RT-PCR and sequencing-based assays. Clinical validation of the technology in a multi-site clinical study is the subject of a separate investigation.

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Section: Introductionmentioning

confidence: 99%

Analytical evaluation of the microarray-based FluChip-8G Influenza A+B Assay

Taylor

Dawson

Blair

et al. 2019

Journal of Virological Methods

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“…It was found that this method had great potential for the rapid typing of high-pathogenicity AIV. In the study conducted by Shi et al [93], a gene chip is established; the method uses the RT-PCR amplification of the H5 H7, and H9 genes and the N1, N2, and M genes, marking it on the aldehyde slides on DNA chips. This method can simultaneously detect many kinds of AIV, including the H5N1 subtype.…”

Section: Gene Chipmentioning

confidence: 99%

Advances in Detection Techniques for the H5N1 Avian Influenza Virus

Fu,

Wang,

et al. 2023

IJMS

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Avian influenza is caused by avian influenza virus infection; the H5N1 avian influenza virus is a highly pathogenic subtype, affecting poultry and human health. Since the discovery of the highly pathogenic subtype of the H5N1 avian influenza virus, it has caused enormous losses to the poultry farming industry. It was recently found that the H5N1 avian influenza virus tends to spread among mammals. Therefore, early rapid detection methods are highly significant for effectively preventing the spread of H5N1. This paper discusses the detection technologies used in the detection of the H5N1 avian influenza virus, including serological detection technology, immunological detection technology, molecular biology detection technology, genetic detection technology, and biosensors. Comparisons of these detection technologies were analyzed, aiming to provide some recommendations for the detection of the H5N1 avian influenza virus.

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“…With the rapid development of microarray technology, an increasing number of veterinary medicine studies have investigated host gene transcriptional responses to infection by various avian viruses[ 11 – 14 ]. REV, avian leucosis virus (ALV), and Marek’s disease virus (MDV) are the main causes of neoplastic diseases in avian hosts.…”

Section: Introductionmentioning

confidence: 99%

Transcriptional Profiling of Host Gene Expression in Chicken Embryo Fibroblasts Infected with Reticuloendotheliosis Virus Strain HA1101

Jiang

Bao

et al. 2015

PLoS ONE

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Reticuloendotheliosis virus (REV), a member of the Gammaretrovirus genus in the Retroviridae family, causes an immunosuppressive, oncogenic and runting-stunting syndrome in multiple avian hosts. To better understand the host interactions at the transcriptional level, microarray data analysis was performed in chicken embryo fibroblast cells at 1, 3, 5, and 7 days after infection with REV. This study identified 1,785 differentially expressed genes that were classified into several functional groups including signal transduction, immune response, biological adhesion and endocytosis. Significant differences were mainly observed in the expression of genes involved in the immune response, especially during the later post-infection time points. These results revealed that differentially expressed genes IL6, STAT1, MyD88, TLRs, NF-κB, IRF-7, and ISGs play important roles in the pathogenicity of REV infection. Our study is the first to use microarray analysis to investigate REV, and these findings provide insights into the underlying mechanisms of the host antiviral response and the molecular basis of viral pathogenesis.

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Development of a DNA microarray-based multiplex assay of avian influenza virus subtypes H5, H7, H9, N1, and N2

Cited by 5 publications

References 14 publications

Analytical evaluation of the microarray-based FluChip-8G Influenza A+B Assay

Analytical evaluation of the microarray-based FluChip-8G Influenza A+B Assay

Advances in Detection Techniques for the H5N1 Avian Influenza Virus

Transcriptional Profiling of Host Gene Expression in Chicken Embryo Fibroblasts Infected with Reticuloendotheliosis Virus Strain HA1101

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