Development of a Duplex Digital PCR Method to Quantify Five Genetically Modified Soybean Events

Long, Likun; Wang, Yan; He, Yong; Li, Dong; Xing, Zhenjuan; Li, Cong-Cong; Xia, Wei; Li, Feiwu

doi:10.1007/s12161-021-02104-2

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Number Of Plants Needed To Construct a Saturated Watermelon ...1

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2024

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Cited by 11 publications

(2 citation statements)

References 22 publications

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“…All primers and probes (Supplemental Table 40) were synthesized at Sangon Biotech (Shanghai, China). For dPCR methods, refer to Long et al (2021), with modifications.…”

Section: Number Of Plants Needed To Construct a Saturated Watermelon ...mentioning

confidence: 99%

A telomere-to-telomere gap-free reference genome of watermelon and its mutation library provide important resources for gene discovery and breeding

Deng¹,

Liu²,

Zhang

et al. 2022

Molecular Plant

116

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“…All primers and probes (Supplemental Table 40) were synthesized at Sangon Biotech (Shanghai, China). For dPCR methods, refer to Long et al (2021), with modifications.…”

Section: Number Of Plants Needed To Construct a Saturated Watermelon ...mentioning

confidence: 99%

A telomere-to-telomere gap-free reference genome of watermelon and its mutation library provide important resources for gene discovery and breeding

Deng¹,

Liu²,

Zhang

et al. 2022

Molecular Plant

116

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“…The regular PCR instrument is used to amplify the individual sub-reactions and Poisson statistics are used to determine the concentration of the target sequence [ 19 ]. The use of primers and probes for one target and one reference gene in the same PCR (duplex dPCR) has been routinely used for the detection and quantification of GM events [ 20 , 21 , 22 ]. However, the detection and quantification of one GM event at a time is not efficient.…”

Section: Introductionmentioning

confidence: 99%

Increasing the Efficiency of Canola and Soybean GMO Detection and Quantification Using Multiplex Droplet Digital PCR

Demeke¹,

Lee²,

Eng³

2022

Biology

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The number of genetically modified (GM) events for canola, maize, and soybean has been steadily increasing. Real-time PCR is widely used for the detection and quantification of individual GM events. Digital PCR (dPCR) has also been used for absolute quantification of GM events. A duplex dPCR assay consisting of one reference gene and one GM event has been carried out in most cases. The detection of more than one GM event in a single assay will increase the efficiency of dPCR. The feasibility of detection and quantification of two, three, and four GM canola and soybean events at the same time was investigated at 0.1%, 1%, and 5% levels using the QX200 Droplet Digital PCR (ddPCR) system. The reference gene assay was carried out on the same plate but in different wells. For some of the assays, optimization of the probe concentrations and labels was needed for successful ddPCR. Results close to the expected result were achieved for duplex, triplex, and tetraplex ddPCR assays for GM canola events. Similar ddPCR results were also achieved for some GM soybean events with some exceptions. Overall, absolute quantification of up to four GM events at the same time improves the efficiency of GM detection.

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A high-throughput screening method for GM soybean events based on single universal primer multiplex PCR and capillary electrophoresis

Dong,

Li,

Xing

et al. 2024

J Consum Prot Food Saf

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Development of a Duplex Digital PCR Method to Quantify Five Genetically Modified Soybean Events

Cited by 11 publications

References 22 publications

A telomere-to-telomere gap-free reference genome of watermelon and its mutation library provide important resources for gene discovery and breeding

A telomere-to-telomere gap-free reference genome of watermelon and its mutation library provide important resources for gene discovery and breeding

Increasing the Efficiency of Canola and Soybean GMO Detection and Quantification Using Multiplex Droplet Digital PCR

A high-throughput screening method for GM soybean events based on single universal primer multiplex PCR and capillary electrophoresis

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