Development of a fast capillary electrophoresis method for determination of carbohydrates in honey samples

Abstract

In this study, the determination of fructose, glucose and sucrose by capillary electrophoresis (CE) was investigated. The tendency of the analyte to undergo electromigration dispersion and the buffer capacity were evaluated using the Peakmaster(®) software and considered in the optimization of the background electrolyte, which was composed of 20 mmol L(-1) sorbic acid, 0.2 mmol L(-1) CTAB and 40 mmol L(-1) NaOH at pH 12.2. Under optimal CE conditions, the separation of the substances investigated was achieved in less than 2 min. The detection limits for the three analytes were in the range of 0.022 and 0.029 g L(-1) and precision measurements within 0.62-4.69% were achieved. The proposed methodology was applied in the quantitative analysis by direct injection of in honey samples to determine the main sugars presents. The samples were previously dissolved in deionized water and filtered with no other sample treatment. The mean values for fructose, glucose and sucrose were in the ranges of 33.65-45.46 g 100g(-1), 24.63-35.06 g 100g(-1) and <0.22-1.32 g 100g(-1), respectively. The good analytical performance of the method makes it suitable for implementation in food laboratories for the routine analysis of honey samples.

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

“…As a result, levels of the three saccharides were quantified in all samples. Same saccharides were determined in seven honey samples . The quantitative results revealed that fructose was the major sugar in honey samples (33.65–45.46 g /100 g) followed by glucose (22.34–35.39 g/100 g).…”

Recent advances in the application of capillary electromigration methods for food analysis and Foodomics

“…An option to solve the lack of chromophores is the use of indirect UV detection. For instance, Rizelio et al developed a CE methodology for the determination of fructose, glucose, and sucrose in seven multifloral honey samples. A satisfactory and rapid separation (less than 2 min) was achieved using a BGE comprised of 20 mM sorbic acid, 0.2 mM CTAB, and 40 mM sodium hydroxide (pH 12.2).…”

Double-chained cationic surfactant modification of SU-8/Pyrex® microchips for electrochemical sensing of carboxylic ferrocene after reverse electrophoresis

“…Cetyltrimethylammonium bromide (CTAB), a single-chain surfactant, has been extensively studied as dynamic modifier to obtain a reversal of the EOF in CE [38][39][40] and ME [41][42][43]. Thus, borosilicate glass microchips were modified with CTAB for the determination of inorganic ions [41] , or arsenic species [42] as well as a PMMA microchip for the determination of urine markers [43].…”

“…Reversed EOF has been used in capillary and microchip electrophoresis for the separation of small inorganic and organic anions [33], acidic plant hormones [34], basic proteins [35], and peptides [36]. Apart from changing the polarity of the high-voltage electrodes, a positive charge should be generated on the wall and dynamic coating with cationic surfactants has been employed with this aim [37].Cetyltrimethylammonium bromide (CTAB), a single-chain surfactant, has been extensively studied as dynamic modifier to obtain a reversal of the EOF in CE [38][39][40] and ME [41][42][43]. Thus, borosilicate glass microchips were modified with CTAB for the determination of inorganic ions [41] , or arsenic species [42] as well as a PMMA microchip for the determination of urine markers [43].…”