Development of a Flow Cytometric Method To Analyze Subpopulations of Bacteria in Probiotic Products and Dairy Starters

Bunthof, C.J.; Abee, Tjakko

doi:10.1128/aem.68.6.2934-2942.2002

Cited by 128 publications

(99 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…[3,23] In vitro survival studies recommended 10 9 -10 10 CFU daily dose for probiotic effects since 10 6 CFU/ml might be insufficient as not all bacteria reach the intestines alive. [24] The present study identified a range of 2.2-3.0 × 10 10 CFU per 80-ml bottle of Philippine Yakult monitored for a period of 31 days, which is comparable with the identified approximately 3.0 × 10 10 CFU per 65-ml recommended dose bottle of Yakult [25] containing at least 1.0 × 10 8 CFU/ml at the expiry date. [11] Plate counting has been employed in most researches for the assessment of probiotic viability.…”

Section: Discussionsupporting

confidence: 55%

Viability kinetics of Lactobacillus casei Shirota strain in a commercial fermented milk drink during refrigerated storage

Sumalapao¹,

Mesina²,

Cabrera³

et al. 2017

Natl J Physiol Pharm Pharmacol

View full text Add to dashboard Cite

Background: One of the most important parameters in a commercial fermented milk drink containing probiotics is the viability of the microorganisms. Aims and Objectives: This study described the viability kinetics of Lactobacillus casei Shirota strain in a commercial fermented milk drink during refrigerated storage using nonlinear models. Materials and Methods: Viability of L. casei Shirota strain was monitored during refrigerated storage using standard bacterial plate count method. Several nonlinear mathematical models such as the zero-order, first-order, and second-order kinetic equations were employed in describing the population dynamics. Best-fit models were selected based on prescribed criteria including sum of squares of the error, p-value, and coefficient of determination. Results: The viable counts of L. casei Shirota strain in the fermented milk samples stored under refrigerated conditions decreased from 3.73 × 10 8 colony forming units per ml (CFU/ml) to 2.70 × 10 8 CFU/ml when monitored every 3-4 days interval over 10 different monitoring points within the product's indicated shelf life. The counts significantly differed between the monitoring points that were at least 14 days apart. The lowest viable count was still within the recommended therapeutic dose. The decrease in the bacterial population behaved under a second-order kinetic relationship. Conclusion: The viability of L. casei Shirota strain in a commercial fermented milk product during refrigerated storage is governed in accordance with the second-order kinetic mechanism.

show abstract

Section: Discussionsupporting

confidence: 55%

Viability kinetics of Lactobacillus casei Shirota strain in a commercial fermented milk drink during refrigerated storage

Sumalapao¹,

Mesina²,

Cabrera³

et al. 2017

Natl J Physiol Pharm Pharmacol

View full text Add to dashboard Cite

show abstract

“…This technique has demonstrated its potential as a means to assess the physiological state of damaged cells (Ueckert et al 1997). FCM has been successfully used in several studies to assess the viability of microbial cells in probiotic products and dairy starters (Bunthof and Abee 2002), starved cultures Kaprelyants et al 1996), cells having been exposed to antibiotics (Novo et al 2000) or high hydrostatic pressure (Ritz et al 2001).…”

Section: Introductionmentioning

confidence: 99%

Viability and physiological state transitions of Rhizopus oligosporus sporangiospores in tempe starter culture

Thành

Rombouts

Nout

2006

Antonie van Leeuwenhoek

View full text Add to dashboard Cite

The viability and various physiological characteristics of individual sporangiospores of Rhizopus oligosporus in tempe starter cultures that had been stored for 8, 10, 16 and 30 months were examined by flow cytometry in combination with fluorescent dyes. Besides live, dead, and dormant spores we distinguished a category of sublethally damaged spores. Results indicated that the shelf-life of tempe starters was not limited by the death of spores, but by sublethal damage to spores as well as by dormancy which can be overcome by resuscitation, respiratory activation. During storage, the number of dormant and sublethally damaged spores increased: the longer the starter cultures were stored, the less dormant spores could still be activated. In contrast, the transition from sublethally damaged (spores that are not able to transform cFDA and emit green fluorescence except by activation treatment) to activated spores did not decrease with longer storage. However, after very long (30 months) storage, sublethally damaged spores could still be activated but could not germinate anymore. The shelf-life of spores in tempe starter is related to the physiological state of spores being sublethally damaged; a mechanism of physiological state transitions of R. oligosporus sporangiospores is proposed.

show abstract

“…Such DNA-intercalating dyes are able to bind upon exposure to bright visible light to DNA and, consequently, to inhibit PCR amplification of the DNA which is free or inside the bacterial cells with the damaged membrane. Although probiotic bacteria in the products are represented in different stages not only as viable or dead (Bunthof and Abee, 2002), the most important criterion for distinguishing between viable and irreversibly damaged cells is membrane integrity. The treatment of bacteria with EMA as a promising tool of DNA-based differentiation between viable and dead pathogenic bacteria was first proposed by Nogva et al in 2003(Nogva et al, 2003.…”

Section: Viability Determination Of Probiotics By Pcr-based Methodsmentioning

confidence: 99%

Application of PCR-Based Methods to Dairy Products and to Non-Dairy Probiotic Products

Monnet¹,

Matijašić²

2012

Polymerase Chain Reaction

View full text Add to dashboard Cite

Development of a Flow Cytometric Method To Analyze Subpopulations of Bacteria in Probiotic Products and Dairy Starters

Abstract: Flow cytometry (FCM) is a rapid and

Cited by 128 publications

References 38 publications

Viability kinetics of Lactobacillus casei Shirota strain in a commercial fermented milk drink during refrigerated storage

Viability kinetics of Lactobacillus casei Shirota strain in a commercial fermented milk drink during refrigerated storage

Viability and physiological state transitions of Rhizopus oligosporus sporangiospores in tempe starter culture

Application of PCR-Based Methods to Dairy Products and to Non-Dairy Probiotic Products

Contact Info

Product

Resources

About