Development of a flow-free magnetic actuation platform for an automated microfluidic ELISA

Coarsey, Chad; Coleman, Benjamin; Kabir, Alamgir; Sher, Mazhar; Asghar, Waseem

doi:10.1039/c8ra07607c

Cited by 45 publications

(32 citation statements)

References 34 publications

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“…The DEP module was integrated into a microfluidic chip to detect DENV with fluorescence immunosensing (Iswardy et al, 2017). 3C) (Coarsey et al, 2019). Also, an automated magnetic bead-based microfluidic ELISA method for detection of HIV-1 based on p24 capsid antigen detection was developed (Fig.…”

Section: On-chip Immunoassaymentioning

confidence: 99%

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Recent advances in lab-on-a-chip technologies for viral diagnosis

Zhu

Fohlerová

Pekárek

et al. 2020

Biosensors and Bioelectronics

195

146

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A B S T R A C TThe global risk of viral disease outbreaks emphasizes the need for rapid, accurate, and sensitive detection techniques to speed up diagnostics allowing early intervention. An emerging field of microfluidics also known as the lab-on-a-chip (LOC) or micro total analysis system includes a wide range of diagnostic devices. This review briefly covers both conventional and microfluidics-based techniques for rapid viral detection. We first describe conventional detection methods such as cell culturing, immunofluorescence or enzyme-linked immunosorbent assay (ELISA), or reverse transcription polymerase chain reaction (RT-PCR). These methods often have limited speed, sensitivity, or specificity and are performed with typically bulky equipment. Here, we discuss some of the LOC technologies that can overcome these demerits, highlighting the latest advances in LOC devices for viral disease diagnosis. We also discuss the fabrication of LOC systems to produce devices for performing either individual steps or virus detection in samples with the sample to answer method. The complete system consists of sample preparation, and ELISA and RT-PCR for viral-antibody and nucleic acid detection, respectively. Finally, we formulate our opinions on these areas for the future development of LOC systems for viral diagnostics.

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Section: On-chip Immunoassaymentioning

confidence: 99%

“…(B) 3D bead-based microfluidic chip for infection disease detection (Krejcova et al, 2014). (C) A flow-free magnetic actuation platform for microfluidic ELISA test (Coarsey et al, 2019). (D) Paper-based microfluidic test for ZIKV NS1 and DENV detection (Bedin et al, 2017).…”

Section: Na Amplification In Microfluidic Systemsmentioning

confidence: 99%

Recent advances in lab-on-a-chip technologies for viral diagnosis

Zhu

Fohlerová

Pekárek

et al. 2020

Biosensors and Bioelectronics

195

146

View full text Add to dashboard Cite

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“…We fabricated a three-layer microchip using a cheaper non-lithographic method by laser cutting (Universal Laser) polymethylmethacrylate (PMMA) and double-sided adhesive (DSA) materials using an optimized design previously published [20]. The top layer (750 µm thick) has 0.4 mm diameter inlets and outlets to fit the pipette tips to allow easy sample loading.…”

Section: Microfluidic Chip Designmentioning

confidence: 99%

“…An Arduino-controlled in-house-built magnetic actuation platform [20] was used to facilitate the automation of the developed assay (Figure 1a,b). The platform consisted of two parts including an Arduino controlling unit and the 3D printed platform, which could accommodate the microfluidic chip.…”

Section: Automated M-elisa On Chipmentioning

confidence: 99%

“…This can be beneficial in certain parts of the world where accessibility and cost are significant factors. The reduction in time is achieved through the increase of surface area with the magnetic beads [20]. The developed device is also automated, which can create a significant advantage and allow an individual to run multiple assays at a time.…”

Section: Introductionmentioning

confidence: 99%

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Development of a Flow-Free Automated Colorimetric Detection Assay Integrated with Smartphone for Zika NS1

Kabir

Zilouchian²,

Sher

et al. 2020

Diagnostics

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The Zika virus (ZIKV) is an emerging flavivirus transmitted to humans by Aedes mosquitoes that can potentially cause microcephaly, Guillain–Barré Syndrome, and other birth defects. Effective vaccines for Zika have not yet been developed. There is a necessity to establish an easily deployable, high-throughput, low-cost, and disposable point-of-care (POC) diagnostic platform for ZIKV infections. We report here an automated magnetic actuation platform suitable for a POC microfluidic sandwich enzyme-linked immunosorbent assay (ELISA) using antibody-coated superparamagnetic beads. The smartphone integrated immunoassay is developed for colorimetric detection of ZIKV nonstructural protein 1 (NS1) antigen using disposable chips to accommodate the reactions inside the chip in microliter volumes. An in-house-built magnetic actuator platform automatically moves the magnetic beads through different aqueous phases. The assay requires a total of 9 min to automatically control the post-capture washing, horseradish peroxidase (HRP) conjugated secondary antibody probing, washing again, and, finally, color development. By measuring the saturation intensity of the developed color from the smartphone captured video, the presented assay provides high sensitivity with a detection limit of 62.5 ng/mL in whole plasma. These results advocate a great promise that the platform would be useful for the POC diagnosis of Zika virus infection in patients and can be used in resource-limited settings.

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Emerging Trends and Future Prospects in Microfluidic Systems for Prevention and Diagnosis of Infection Pathogens

Dabbagh Moghaddam,

Rafiee,

Setayeshfar

et al. 2024

Adv Materials Technologies

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Infectious diseases caused by bacteria, viruses, fungi, and parasites pose a significant societal challenge. In response, scientists are developing advanced technology to enhance the prevention, diagnosis, and treatment of such diseases. One such promising technology is microfluidic systems, which are utilized in organ‐on‐a‐chip systems to replicate the microenvironments of organs. These systems have potential applications in drug screening, disease modeling, and personalized medicine. This review provides an overview of recent advances in organ‐on‐a‐chip platforms and their potential for preventing and diagnosing various infections. After discussing traditional techniques employed in studying infectious diseases, the role of microfluidic platforms in detecting infections is delved in. It is expound on advanced microfluidic‐based strategies for infection diagnosis, such as the polymerase chain reaction‐based microfluidic devices, enzyme linked immunosorbent assay‐based microfluidic devices, hierarchical nanofluidic molecular enrichment systemand µWestern blotting‐based microfluidic devices, and smartphone‐based microfluidic devices. Additionally, future research challenges and perspectives are discussed on microfluidic systems in biomedical and regenerative medicine applications. Consequently, microfluidic platforms have the potential to serve as fundamental frameworks for understanding infectious diseases, thereby leading to personalized regenerative medicine. hierarchical nanofluidic molecular enrichment system

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Development of a flow-free magnetic actuation platform for an automated microfluidic ELISA

Abstract: A flow-free device is developed for automated and rapid ELISA testing at the point-of-care settings.

Cited by 45 publications

References 34 publications

Recent advances in lab-on-a-chip technologies for viral diagnosis

Recent advances in lab-on-a-chip technologies for viral diagnosis

Development of a Flow-Free Automated Colorimetric Detection Assay Integrated with Smartphone for Zika NS1

Emerging Trends and Future Prospects in Microfluidic Systems for Prevention and Diagnosis of Infection Pathogens

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