Development of a gas chromatography/mass spectrometry method to quantify several urinary monohydroxy metabolites of polycyclic aromatic hydrocarbons in occupationally exposed subjects

Campo, Laura; Rossella, F.; Fustinoni, Silvia

doi:10.1016/j.jchromb.2008.10.017

Cited by 97 publications

(58 citation statements)

References 39 publications

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“…We determined 12 urinary PAH metabolites, including 1-, 2-hydroxynaphthalene, 2-, 9-hydroxyfluorene, 1-hydroxypyrene, 1-, 2-, 3-, 4-, 9-hydroxyphenanthrene, 6-hydroxychrysene, and 3-hydroxybenzo[a]pyrene by gas chromatography-mass spectrometry, which was reported in detail previously (32) with minor modifications as described in our previous study (33). We failed to detect 6-hydroxychrysene and 3-hydroxybenzo[a]pyrene because their concentrations were less than the limits of detection (LOD).…”

Section: Determination Of Urinary Pah Metabolitesmentioning

confidence: 99%

Associations between 25 Lung Cancer Risk–Related SNPs and Polycyclic Aromatic Hydrocarbon–Induced Genetic Damage in Coke Oven Workers

Dai

Deng

Wang

et al. 2014

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Background: Genome-wide association studies (GWAS) have identified multiple single-nucleotide polymorphisms (SNP) associated with lung cancer. However, whether these SNPs are associated with genetic damage, a crucial event in cancer initiation and evolution, is still unknown. We aimed to establish associations between these SNPs and genetic damage caused by the ubiquitous carcinogens, polycyclic aromatic hydrocarbons (PAH).Methods: We cross-sectionally investigated the associations between SNPs from published GWAS for lung cancer in Asians and PAH-induced genetic damage in 1,557 coke oven workers in China. Urinary PAH metabolites, plasma benzo[a]pyrene-r-7,t-8,c-10-tetrahydrotetrol-albumin (BPDE-Alb) adducts, urinary 8-hydroxydeoxyguanosine (8-OHdG), and micronuclei (MN) frequency were determined by gas chromatography-mass spectrometry, sandwich ELISA, high-performance liquid chromatography, and cytokinesis-block micronucleus assay, respectively.Results: 13q12.12-rs753955C was suggestively associated with elevated 8-OHdG levels (P ¼ 0.003). Higher 8-OHdG levels were observed in individuals with rare allele homozygotes (CC) than in TT homozygotes (b, 0.297; 95% confidence interval, 0.124-0.471; P ¼ 0.001). 9p21-rs1333040C, 10p14-rs1663689G, and 15q25.1-rs3813572G were significantly associated with lower MN frequency (P values were 0.002, 0.001, and 0.005, respectively). 10p14-rs1663689G polymorphism downregulated the relationship of the total concentration of PAH metabolites to 8-OHdG levels (P interaction ¼ 0.002). TERT-rs2736100G and VTI1A-rs7086803A aggravated the relationship of BPDE-Alb adducts to MN frequency, whereas BPTF-rs7216064G attenuated that correlation (all P interaction < 0.001).Conclusions: Lung cancer risk-associated SNPs and their correlations with PAH exposure were associated with 8-OHdG levels and MN frequency.Impact: Lung cancer risk-associated SNPs might influence one's susceptibility to genetic damage caused by PAHs. Cancer Epidemiol Biomarkers Prev; 23(6); 986-96. Ó2014 AACR.

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Section: Determination Of Urinary Pah Metabolitesmentioning

confidence: 99%

Associations between 25 Lung Cancer Risk–Related SNPs and Polycyclic Aromatic Hydrocarbon–Induced Genetic Damage in Coke Oven Workers

Dai

Deng

Wang

et al. 2014

Cancer Epidemiology, Biomarkers &Amp; Prevention

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“…Assays have been developed using gas chromatography-mass spectrometry or high performance liquid chromatography with fluorescence detection to simultaneously measure OHPAHs derived from multiple PAHs [36][37][38][39][40][41]. More recently, liquid chromatography-tandem mass spectrometry (LC-MS/MS) has been introduced to eliminate the need for derivatization of OHPAHs for gas chromatography and to overcome the lack of specificity in fluorescence detection-based methods [42].…”

Section: Introductionmentioning

confidence: 99%

Using liquid chromatography–tandem mass spectrometry to quantify monohydroxylated metabolites of polycyclic aromatic hydrocarbons in urine

Onyemauwa

Rappaport

Sobus

et al. 2009

Journal of Chromatography B

114

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“…liquid chromatography (HPLC) systems with fluorescence detection [7,8], liquid chromatography-mass spectrometry (LC-MS) [9] and gas chromatography-mass spectrometry (GC-MS) [10]. However, there are still some disadvantages, such as time-consuming pretreatment of the sample prior to chromatography analysis [11,12].…”

Section: Introductionmentioning

confidence: 99%

G-quadruplex based impedimetric 2-hydroxyfluorene biosensor using hemin as a peroxidase enzyme mimic

Liang

Liu

2015

Microchim Acta

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We describe a sensitive and selective biosensor for the environmental metabolite 2-hydroxyfluorene (2-HOFlu). It is based on electrochemical impedance spectroscopy and was obtained by assembling a thiolated single-stranded DNA on a gold electrode via S-Au covalent bonding. It is then transformed to a K + -stabilized G-quadruplex-hemin complex which exhibits peroxidase-like activity to catalyze the oxidation of 2-HOFlu by H 2 O 2 . This results in the formation of insoluble products that are precipitated on the gold electrode. As a result, the charge transfer resistance (R CT ) between the solution and the electrode surface is strongly increased within 10 min as demonstrated by using the ferro/ferricyanide system as a redox probe. The difference in the charge transfer resistances (ΔR CT ) before and after incubation of the DNA film with 2-HOFlu and H 2 O 2 serves as the signal for the quantitation of 2-HOFlu with a 1.2. nM detection limit in water of pH 7.4. The assay is highly selective over other selected fluorene derivatives. It was exploited to determine 2-HOFlu in spiked lake water samples where it displayed a detection limit of 3.6 nM. Conceivably, this method has a wide scope in that it may be applied to other analytes for which respective G-quadruplexes are available.

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Development of a gas chromatography/mass spectrometry method to quantify several urinary monohydroxy metabolites of polycyclic aromatic hydrocarbons in occupationally exposed subjects

Cited by 97 publications

References 39 publications

Associations between 25 Lung Cancer Risk–Related SNPs and Polycyclic Aromatic Hydrocarbon–Induced Genetic Damage in Coke Oven Workers

Associations between 25 Lung Cancer Risk–Related SNPs and Polycyclic Aromatic Hydrocarbon–Induced Genetic Damage in Coke Oven Workers

Using liquid chromatography–tandem mass spectrometry to quantify monohydroxylated metabolites of polycyclic aromatic hydrocarbons in urine

G-quadruplex based impedimetric 2-hydroxyfluorene biosensor using hemin as a peroxidase enzyme mimic

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