Development of a gold immunochromatographic strip for the rapid detection of 3-amino-5-morpholinomethyl-2-oxazolidinone (AMOZ) in catfish

Abstract

In this study, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a gold immunochromatographic assay were developed to detect the furaltadone metabolite, 3-amino-5morpholinomethyl-2-oxazolidinone (AMOZ). The detection of AMOZ was based on the AMOZ derivative 3-([2-nitrophenyl] methyleneamino)-5-morpholinomethyl-2-oxazolidinone (2-NPAMOZ). The monoclonal anti-2-NPAMOZ antibody 4G11, was prepared and exhibited a 50%-inhibitory concentration (IC 50) of 0.049 ng/mL. Its cross-reactivity with structurally related analogues was less than 3%. This immunochromatographic assay was assembled for the rapid screening of AMOZ in catfish samples. The strip assay had a cutoff value of 0.1 μg/kg and a limit of detection (LOD) of 0.009 μg/kg. Analysis of AMOZ in catfish indicated that the results from the strip assay were highly comparable to results obtained using ic-ELISA and LC-MS/MS. Therefore, the colloidal gold (CG) immunoassay is a sensitive screening method for the detection of AMOZ residues in catfish samples.

Immunological quantitative detection of dicofol in medicinal materials

“…Next, the colloidal gold labeled mAb was prepared according to earlier reports. [25][26][27][28] Briefly, 10 mL of the colloidal gold particle solution was adjusted using K 2 CO 3 (0.1 mol L −1 ) to pH 8.0 and mixed well. Then, an appropriate amount of purified mAb was diluted to 0.2 mg mL −1 with boric acid buffer (0.02 mol L −1 , pH 8.0), 50 μL of this was added dropwise to the colloidal gold particle solution and fully mixed, and the mixture was placed at room temperature for 40 min.…”

Quantum dot nanobeads as multicolor labels for simultaneous multiplex immunochromatography detection of four nitrofuran metabolites in aquatic products

“…All samples were packaged foods and stored at a frozen condition at the time of purchase. The sample preparation method is according to a previously reported method (Lu et al, 2020). The muscle tissues of the testing samples were minced and hydrolyzed with HCl, and 2-NBA was added for derivatization.…”

“…To date, commercially available test strips have been developed based on colloidal gold nanoparticles as reporters for colorimetric detection. However, gold nanoparticle test strips have limited detection sensitivity and lack quantitative detection capability (Lu et al, 2020). Recently, uorescent nanomaterials have attracted signi cant research interest in the eld of analysis and detection because of their high luminescence (Nguyen et al, 2020;You et al, 2019).…”

Quantitative and rapid detection of spinosad and spinetoram by a gold nanoparticle-based immunostrip

“…These methods, including indirect competitive enzyme-linked immunosorbent assay (ic-ELISA), immunochromatographic strips, and immunomagnetic beads, have been successfully developed for the detection of pesticides, antibiotics, and heavy metals. [24][25][26] Lee et al 27 reported a uorescence excitation transfer immunoassay for the analysis of spinosyn A in wastewater, with a limit of detection (LOD) of 0.01 ng g À1 . Young et al 28 detected SPI residues in 34 matrices using a magnetic particle-based immunoassay test kit, with a limit of quantitation of 0.1-50 ng g À1 .…”