Development of a graphene oxide nanosheet and double-stranded DNA structure based fluorescent “signal off” aptasensor for ochratoxin A detection in malt

Zhang, Qing; Kang, Linzhi; Yue, Pengfei; Shi, Linchun; Wang, Meng; Zhou, Lidong; Zhao, Huimin; Kong, Weijun

doi:10.1016/j.fochx.2022.100308

Cited by 9 publications

(3 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There are numerous approaches where GO operates as a support nanomaterial for detecting DNA, including electrochemical [ 13 , 14 ], field-effect transistors [ 15 ], and fluorescent biosensor applications [ 16 , 17 ]. Among these, fluorescent base biosensors have been of particular interest due to their enhanced capabilities, such as low cost, simplicity, high sensitivity, superior selectivity, fast response, and multiple analyses [ 18 ]. In previous studies, GO has been utilized to retain low ssDNA concentrations through the fluorescence “turn off” quenching mechanism and as a platform for targeted ssDNA identifications in protein-containing media.…”

Section: Introductionmentioning

confidence: 99%

Graphene Oxide/Nitrocellulose Non-Covalent Hybrid as Solid Phase for Oligo-DNA Extraction from Complex Medium

Toader,

Nitu,

Ionita

2023

Molecules

View full text Add to dashboard Cite

A nitrocellulose–graphene oxide hybrid that consists of a commercially nitrocellulose (NC) membrane non-covalently modified with graphene oxide (GO) microparticles was successfully prepared for oligonucleotide extraction. The modification of NC membrane was confirmed by Fourier Transform Infrared Spectroscopy (FTIR), which highlighted the principal absorption bands of both the NC membrane at 1641, 1276, and 835 cm−1 (NO2) and of GO in the range of 3450 cm−1 (CH2-OH). The SEM analysis underlined the well-dispersed and uniform coverage of NC membrane with GO, which displayed thin spider web morphology. The wettability assay indicated that the NC–GO hybrid membrane exhibited slightly lower hydrophilic behavior, with a water contact angle of 26.7°, compared to the 15° contact angle of the NC control membrane. The NC–GO hybrid membranes were used to separate oligonucleotides that had fewer than 50 nucleotides (nt) from complex solutions. The features of the NC–GO hybrid membranes were tested for extraction periods of 30, 45, and 60 min in three different complex solutions, i.e., an aqueous medium, an α-Minimum Essential Medium (αMEM), and an αMEM supplemented with fetal bovine serum (FBS). The oligonucleotides were desorbed from the surface of the NC–GO hybrid membrane using Tris-HCl buffer with a pH of 8.0. Out of the three media utilized, the best results were achieved after 60 min incubation of the NC–GO membranes in αMEM, as evidenced by the highest fluorescence emission of 294 relative fluorescence units (r.f.u.). This value corresponded to the extraction of approximately 330–370 pg (≈7%) of the total oligo-DNA. This method is an efficient and effortless way to purify short oligonucleotides from complex solutions.

show abstract

Section: Introductionmentioning

confidence: 99%

Graphene Oxide/Nitrocellulose Non-Covalent Hybrid as Solid Phase for Oligo-DNA Extraction from Complex Medium

Toader,

Nitu,

Ionita

2023

Molecules

View full text Add to dashboard Cite

show abstract

“…The aptamer binds more strongly to the target molecule (OTA) than to the cDNA. 34,35 Upon addition of the target molecule, the cDNA with the fluorescent label is shed.…”

Section: Resultsmentioning

confidence: 99%

Highly sensitive and specific assessment of ochratoxin A in herbal medicines via activator regeneration by electron transfer ATRP

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Due to the π-stacking interaction between the π-ring of GO and the nucleobases of ssDNA, the fluorophore-label on the aptamer loses its fluorescence upon contact with GO [22,23]. Considering their stability and practicability, GO-based fluorescent biosensors have been widely applied in various fields [24][25][26].…”

mentioning

confidence: 99%

Selected ssDNA aptamers–graphene oxide‐based fluorescent biosensor to detect sulfameter in milk

Zhang

Tian

et al. 2023

Luminescence

View full text Add to dashboard Cite

The abuse of sulfameter (SME) in animal husbandry can cause drug resistance and toxic or allergic reactions in humans. Therefore, it is very important to establish a simple, inexpensive, and efficient method for detecting SME in food. In this work, we propose a single fluorescent aptamer/graphene oxide (GO)‐based biosensor to detect SME residues in milk. Aptamers that specifically bind to SME were screened using capture‐SELEX and a ssDNA library immobilized on magnetic beads. The 68 active candidate aptamers were chemically synthesized for specificity and affinity characterization. Among the aptamers, the aptamer sulf‐1 revealed the highest affinity (Kd = 77 ± 15 nM) to SME and was selected to construct a GO‐based fluorescent biosensor for real milk sample detection. Under optimal conditions, the single fluorescent aptasensor had a wide linear range (R2 was 0.997) from 7 to 336 ng/ml and a low detection limit of 3.35 ng/ml that was calculated with a 3SD/slope. The single fluorescent method was also validated using SME‐fortified milk samples, showing average recoveries ranging from 99.01% to 104.60% with a relative standard deviation of less than 3.88%. These results demonstrate that this novel aptamer sensor provides an opportunity for sensitive, convenient, and accurate detection of SME residues in milk.

show abstract

Development of a graphene oxide nanosheet and double-stranded DNA structure based fluorescent “signal off” aptasensor for ochratoxin A detection in malt

Cited by 9 publications

References 29 publications

Graphene Oxide/Nitrocellulose Non-Covalent Hybrid as Solid Phase for Oligo-DNA Extraction from Complex Medium

Graphene Oxide/Nitrocellulose Non-Covalent Hybrid as Solid Phase for Oligo-DNA Extraction from Complex Medium

Highly sensitive and specific assessment of ochratoxin A in herbal medicines via activator regeneration by electron transfer ATRP

Selected ssDNA aptamers–graphene oxide‐based fluorescent biosensor to detect sulfameter in milk

Contact Info

Product

Resources

About