Development of a heptaplex PCR system to analyse X-chromosome STR loci from five Italian population samples. A collaborative study

Bini, Carla; Ceccardi, Stefania; Ferri, Gianmarco; Pelotti, Susi; Alù, Milena; Roncaglia, Enrica; Beduschi, Giovanni; Caenazzo, Luciana; Ponzano, E.; Tasinato, Paola; Turchi, Chiara; Mazzanti, Marta; Tagliabracci, Adriano; Toni, Cristina; Spinetti, Isabella; Domenici, Ranieri; Presciuttini, Silvano

doi:10.1016/s0531-5131(03)01720-5

Cited by 20 publications

(25 citation statements)

References 9 publications

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“…These factors facilitate the X chromosome in being a source of information in human evolution and migration studies [1]. However, a limited number of X chromosome STR polymorphisms have been reported [3][4][5][6][7][8][9][10][11][12]. Gomes et al have described an X-STR decaplex system [11], and based on the results of their report, we developed a 13 X-STR multiplex system that could be amplified in one single polymerase chain reaction (PCR).…”

Section: Introductionmentioning

confidence: 99%

Thirteen X-chromosomal short tandem repeat loci multiplex data from Taiwanese

Hwa

Chang²,

Lee

et al. 2009

Int J Legal Med

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Study results of variations in the X chromosome are useful tools in researching the genetic diversity of human populations and individual identification. We developed a 13 X chromosomal short tandem repeat (STR) multiplex system (DXS6807, DXS8378, DSX9902, DXS7132, DXS9898, DXS6809, DXS6789, DXS7424, DXS101, GATA172D05, HPRTB, DXS8377, DXS7423) amplified in one single polymerase chain reaction. DNA samples of 113 male and 108 female Taiwanese Han subjects were successfully analyzed using this 13 X-STR multiplex system. The distributions of allele frequencies were examined for independence. DXS8377, DXS101, DXS6789, and DXS6809 were found to be the most polymorphic markers in this study. High values of discrimination power and mean exclusion chance without significant evidence of association between these markers were obtained. In conclusion, this 13 X chromosomal STR multiplex system offers considerable forensic efficiency and may be useful in forensic identification casework.

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Section: Introductionmentioning

confidence: 99%

Thirteen X-chromosomal short tandem repeat loci multiplex data from Taiwanese

Hwa

Chang²,

Lee

et al. 2009

Int J Legal Med

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“…[1,4,9,10,15,17,27], used the allele designation according to Hearne and Todd [11] in order to meet exactly the ISFG nomenclature recommendations [2]. As a result, actually two different allele designation modes exhibiting one repeat difference are currently used, and the population data of different studies become comparable only after revision of the HPRTB data.…”

mentioning

confidence: 99%

Nomenclature discrepancies in the HPRTB short tandem repeat

et al. 2009

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“…The first multiplex study with respect to X-STRs analysed nine loci in three different multiplexes which included duplex PCR (DXS6789 and DXS6795), Triplex PCR (DXS7133, DXS9895 and DXS9898) and Quadruplex PCR (DXS6803, DXS8378, GATA164A09, and DXS7132) [60]. In a very short period of time, other studies came up with parallel amplification of three [61], four [62,60], five [63,64], six [65], seven [66], eight [67] ten [15], eleven [68], twelve [69], and thirteen [70,71], X-STR markers. Multiplexes with a greater number of markers are being developed to obtain a higher degree of discrimination.…”

Section: X-chromosomal Strs and Ministrs: Identity Testing And Beyondmentioning

confidence: 99%

Scope of X-Chromosomal MiniSTRs: Current Developments

Israr¹

2014

J Forensic

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The experience of using autosomal short-length amplicon STRs or miniSTRs in profiling of degraded DNA and mass disaster victims is extended into the realm of X-chromosomal (ChrX) STR miniaturization. About half of the total X-STRs are now short-length amplicons and the focus is shifting to using the mini versions of all of them. Joint multiplexing of these loci can be used for solving complex paternity cases and association of mass disaster victims with their families. This technology may herald a new dimension for research in population genetics and evolution. We present an overview of the progress made thus far and the future scope and prospects for X-miniSTRs.

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Development of a heptaplex PCR system to analyse X-chromosome STR loci from five Italian population samples. A collaborative study

Cited by 20 publications

References 9 publications

Thirteen X-chromosomal short tandem repeat loci multiplex data from Taiwanese

Thirteen X-chromosomal short tandem repeat loci multiplex data from Taiwanese

Nomenclature discrepancies in the HPRTB short tandem repeat

Scope of X-Chromosomal MiniSTRs: Current Developments

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