Development of a high-throughput enzyme-linked immunosorbent assay for the routine detection of the carcinogen acrylamide in food, via rapid derivatisation pre-analysis

Preston, Andrew J; Fodey, Terence L.; Elliott, Christopher T.

doi:10.1016/j.aca.2007.12.013

Cited by 41 publications

(25 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A study conducted by Preston et al (2008) reported an immunoassay capable of detecting AA in spiked water samples with enzyme-linked immunosorbent assay (ELISA)-based detection system. The LOD is 65.7 μg/kg.…”

Section: Immunoassaymentioning

confidence: 99%

A Review of Current Methods for the Determination of Acrylamide in Food Products

Tekkeli

Önal

2011

Food Anal. Methods

View full text Add to dashboard Cite

Acrylamide (AA) is a potentially carcinogenic substance which is formed during heating of food products containing carbohydrates and asparagine. It was first detected in food products in 2002. Since that time, several analytical methods have been made available for the quantification of AA in various foods. Starting from the announcement in 2002, occurrence, formation, chemistry, toxicology, and potential health risk in the human diet have been investigated and methods of analysis have been reviewed in many articles. In this paper, current information and analytical methods for the determination of AA have been reviewed.

show abstract

Section: Immunoassaymentioning

confidence: 99%

A Review of Current Methods for the Determination of Acrylamide in Food Products

Tekkeli

Önal

2011

Food Anal. Methods

View full text Add to dashboard Cite

show abstract

“…However, for a major acceptance and implementation of immunoassays, adequate immunoreagents covering relevant chemicals that can potentially be present in food commodities are required. During the last decades, many applications in the food safety area describing the development of immunoassays for the determination of pesticides, hormones, antibiotics, toxins, additives, or industrial residues have been reported [8][9][10][11][12].…”

mentioning

confidence: 99%

Development and validation of a direct competitive monoclonal antibody-based immunoassay for the sensitive and selective analysis of the phytoregulator forchlorfenuron

et al. 2012

View full text Add to dashboard Cite

Forchlorfenuron is a synthetic phytohormone with cytokinin-like activity used worldwide as a plant growth regulator to increase fruit size in a number of crops, mostly in kiwifruit and grape vines. A monoclonal antibody-based enzyme-linked immunosorbent assay (ELISA) for the determination of forchlorfenuron has been characterized and optimized. The selected immunoreagents afforded a highly selective assay with a limit of detection of 10 ng L −1 in buffer.This direct competitive ELISA was validated in terms of trueness, precision, and robustness using both commercial juice and whole fruit samples. Recoveries from fortified kiwifruit juices and white and red musts were between 97 and 131%, with relative standard deviations below 16%. When homogenized whole fruits were analysed after acetonitrile extraction, recoveries between 96 and 113% were found, with a limit of quantification of 5 µg kg −1. The proposed immunoassay was validated by comparison with a reference chromatographic method using fruits from in-field treated grape and kiwifruit vines. Linear regression analysis of ELISA and HPLC-UV determinationsshowed an excellent correlation (r 2 = 0.998), whereas analysis of the slope (0.99 ± 0.01) and of the intercept (−1 ± 3) clearly proved that the developed competitive immunoassay provided results that were statistically comparable to those obtained by the instrumental method for the analysis of CPPU in fruits at trace levels.

show abstract

“…Some methods including chromatography [16][17][18], LC or GC coupled with MS [1,17], CE [20], and ELISA [19] have been reported to be suitable for acrylamide detection, and are summarized in Table 3. Each method has its advantages and limitations in terms of specificity, sensitivity and interference of matrix compound.…”

Section: Merits Of the Developed Methodsmentioning

confidence: 99%

“…A great number of screening methods have been reported to quantitatively analyze acrylamide and its metabolites, such as gas chromatography (GC) with flame ionization or electron capture detectors [15][16][17], liquid chromatography with pulsed amperometric detector (LC-PAD) [18], enzymelinked immunosorbent assay (ELISA) [19], and capillary electrophoresis (CE) [20]. Among them, chromatography methods lack sensitivity and require a relatively complex sample pretreatment.…”

mentioning

confidence: 99%

Determination of Trace Acrylamide in Potato Chip and Bread Crust Based on SPE and HPLC

Zhang

Qiao

et al. 2012

Chromatographia

View full text Add to dashboard Cite

How to determine the quantities of acrylamide produced in foods is an important problem. Here, we report a sensitive method of high performance liquid chromatography based on a solid phase extraction using a reversed phase C 18 column, which was characterized by adsorption experiments with good adsorption ability and rapid adsorption dynamic toward acrylamide. Under optimal condition, the detection limit based on three times the signal-to-noise ratio of the baseline near the analyte peak was 66.0 ng L -1 . With a loading flow rate of 2.0 mL min -1 for loading 100 mL, an enrichment factor of 182 was obtained. The RSD for five replicate extractions of 50 lg L -1 acrylamide was 4.1%. Blank potato samples spiked with acrylamide at 0.125, 0.250, and 0.375 lg g -1 levels were analyzed with recoveries ranging from 88.9 to 89.5%. Moreover, this method was applied to quantitative detection of acrylamide in the crust and potato chip samples.

show abstract

Development of a high-throughput enzyme-linked immunosorbent assay for the routine detection of the carcinogen acrylamide in food, via rapid derivatisation pre-analysis

Cited by 41 publications

References 27 publications

A Review of Current Methods for the Determination of Acrylamide in Food Products

A Review of Current Methods for the Determination of Acrylamide in Food Products

Development and validation of a direct competitive monoclonal antibody-based immunoassay for the sensitive and selective analysis of the phytoregulator forchlorfenuron

Determination of Trace Acrylamide in Potato Chip and Bread Crust Based on SPE and HPLC

Contact Info

Product

Resources

About