Development of a highly efficient, repetitive system of organogenesis in soybean (Glycine max (L.) Merr).

Zhang, Shan; Raemakers, Krit; Tzitzikas, Emmanouil N.; Ma, Zhengqiang; Visser, R.G.F.

doi:10.1007/s00299-005-0971-7

Cited by 37 publications

(29 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It was observed that exogenous cytokinin application alters axillary meristem development, promotes shoot proliferation and regeneration of the cell in meristemic tissues. This phenomenon has been reported previously in cytokinin pretreated seedlings (Shan et al, 2005). The results reported in this study are also in agreement with previous studies suggesting soybean organogenesis by continuous culture on BAP containing medium (Wright et al, 1987) or by culturing in alternative cycle of BAP and TDZ (Barwale and Widholm, 1990) or combination of BAP with auxin mainly 2,4-D or NAA (Tripathi and Tiwari, 2003;Radhakrishnan and Ranjithakumari, 2007) or ZTR for plant regeneration (Kamenicka et al, 1998;Steinitz et al, 2006;Barik et al, 2007) either through organogenesis, callogenesis or protoplast culture.…”

Section: Iv) Rooting (Stage Iv)supporting

confidence: 89%

“…They reported that by the treatment of BAP, the cells in embryonic axes do not remain quiescent and are reprogrammed to produce multiple somatic foci. However, addition of thidiazuron (TDZ) (Franklin et al, 2004;Shan et al, 2005) or kinetin (Ma and Wu, 2008) with BAP has been reported as better combination for embryoid formation from cotyledonary node explants. Radhakrishnan and Ranjithakumari (2007) produced callus from mature half seed's nodal segment using different combinations of BAP and 2,4 dichloro phenoxy acetic acid (2,4-D).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Short communication. Micropropagation of two Pakistani soybean (Glycine max L.) cultivars from cotyledon nodes

Zia

Rizvi

Rehman

et al. 2010

Span. j. agric. res.

View full text Add to dashboard Cite

Soybean is an important oilseed crop throughout the world and there are continuous efforts to improve it through various techniques from field to laboratory. Although soybean has been grown in Pakistan since a long period, there are no or limited factors involving its improvement through biotechnological techniques in Pakistan. This study aimed to optimize a regeneration protocol for two soybean cultivars, NARC-4 and NARC-7, using cotyledonary nodes as explant. Cultivar NARC-4 showed higher percentage of regeneration (88%) and mean number of shoots per explant (7.3 shoot per explant) compared to cv. NARC-7 with maximum frequency of 82% shoot regeneration and maximum mean number of 6.4 shoots per explant. However, variants of cyotkinins in the media had variable effects on regeneration and shoot length. Generally 6-benzylamino purine was better compared to zeatin riboside and kinetin. The results showed that half cotyledon could be effectively used as explant for direct micropropagation in soybean. The results could also be exploited positively for Agrobacterium-mediated genetic transformation.

show abstract

Section: Iv) Rooting (Stage Iv)supporting

confidence: 89%

mentioning

confidence: 99%

Short communication. Micropropagation of two Pakistani soybean (Glycine max L.) cultivars from cotyledon nodes

Zia

Rizvi

Rehman

et al. 2010

Span. j. agric. res.

View full text Add to dashboard Cite

show abstract

“…The highest mean number (7.93) of shoots per explant was obtained from double cotynode explant treated with 2 mg L -1 BA. Shan et al (2005) obtained a maximum of 6 shoots per explant when explants were treated with 0.1 mg L -1 TDZ. Their explants were the cotyledonary nodes, with the epicotyl, hypocotyl and cotyledons removed.…”

Section: Discussionmentioning

confidence: 99%

“…Some reports show the effect of PGRs such as (GA 3 ) on seed germination, seedling growth and crop yield (Malik and Saxena, 1992;Zhang et al, 1999;Hamayun et al, 2010). Few link seed hormonal treatment to multiple shoot induction (Shan et al, 2005;Ma and Wu, 2008). According to our study, although the presence of cytokinins in the germination medium had no effect on the in vitro soybean germination; seedling growth was affected by higher concentrations of BA (2-4.00 mg L -1 ) resulting in development of thicker seedlings which proved to be a better source for excisions of explants for multiple shoot induction in comparison to seedlings developed on PGR free-medium.…”

Section: Discussionmentioning

confidence: 99%

In vitro Multiple Shoot Induction in Soybean

Mangena¹,

Mokwala²,

Nikolova³

2015

IJAB

View full text Add to dashboard Cite

Genetic transformation is a crucial tool for cultivar improvement; however, soybean is regarded as highly recalcitrant and the success of transformation is affected by the explant source and the rate of in vitro shoot regeneration. Multiple shoot induction on soybean (Glycine max L.) cultivar LS 677 was carried out using single and double coty-node explants from soybean seedlings derived from seeds germinated on Murashige and Skoog (MS) culture media containing different concentrations of N 6 -benzyladenine (BA) and BA in combination with kinetin (KIN). Seedlings obtained from cytokinin treatments (2-4 mg L -1 BA) were stronger with thick hypocotyls, reduced epicotyls and primary roots with few or no lateral root formation. These seedlings showed a better potential as a source for excision of explants than the thin, elongated control seedlings grown on MS medium without cytokinins. The highest number of multiple shoots, from both explant types, was obtained on MS media supplemented with 1.57 and 2.00 mg L -1 BA. The induced shoots were efficiently elongated and rooted on MS medium without plant growth regulators (PGRs). Rooted plantlets were acclimatised under controlled conditions with 70% success and produced viable seeds. The results of this study indicate that the soybean double coty-node explants derived from cytokinin treated seedlings could be a better explant source for in vitro shoot multiplication than the single coty-node explants.

show abstract

“…They also reported that BAP, the cells in embryonic axes do not remain quiescent and are reprogrammed to produce multiple somatic foci. In addition of TDZ (thidiazuron) or Kn + BAP showed better performance on embryoids formation from cotyledonary node in soybean (Shan et al 2005, Ma andWu 2008).…”

Section: Introductionmentioning

confidence: 99%

Embryogenic Callus Induction and Efficient Plant Regeneration in Three Varieties of Soybean (Glycine max)

Islam¹,

Islam²,

Hossain³

et al. 2017

Plant Tissue Cult. & Biotech.

View full text Add to dashboard Cite

An efficient callus induction and plant regeneration system has been developed using three local soybean (Glycine max L.) varieties. All the varieties showed good callusing (78.30 -88.80%) from shoot tip (ST) in MS + 3.0 mg/l 2,4-D + 1.0 mg/l BAP. Best callus induction and embryo formation were recorded in T5 for BS-6 as 88.80 and 81.20%, respectively from ST. In case of cotyledonary node (CN) the maximum callus induction (82.40%) and embryo formation (74.20%) were recorded also in T5 for BS-6. Highest frequency (79.40%) of plant regeneration was recorded where ST were used and cultured in MS supplemented with 2.5 mg/l BAP + 1.0 mg/l NAA as well as 76.30% from CN in BS-6. The length of shoot was observed 5.32 and 4.62 cm, respectively from ST and CN for BS-6 with the same medium composition. It was observed that half strength of MS + 2.0 mg/l IBA showed best rooting (9.04). Among the genotypes BS-6 proved to be best explants than ST that exhibited better performance on callus induction and green plant regeneration for all parameters.

show abstract

Development of a highly efficient, repetitive system of organogenesis in soybean (Glycine max (L.) Merr).

Cited by 37 publications

References 23 publications

Short communication. Micropropagation of two Pakistani soybean (Glycine max L.) cultivars from cotyledon nodes

Short communication. Micropropagation of two Pakistani soybean (Glycine max L.) cultivars from cotyledon nodes

In vitro Multiple Shoot Induction in Soybean

Embryogenic Callus Induction and Efficient Plant Regeneration in Three Varieties of Soybean (Glycine max)

Contact Info

Product

Resources

About