Development of a highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) for the detection of phenylethanolamine A in tissue and feed samples and confirmed by liquid chromatography tandem mass spectrometry (LC–MS/MS)

Cao, Biyun; He, Guangzhao; Yang, Hong; Chang, Huafang; Li, Shuqun; Deng, Anping

doi:10.1016/j.talanta.2013.06.026

Cited by 62 publications

(33 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The concentrations of interfering species of 1 ng mL À1 , 25 ngmL À1 and 100 ng mL À1 were detected, respectively. Comparing with CLB (shown in Figure 5A), the effect of RAC and PA could be neglectable, while CLB exhibited cross-reactivity about 24.3 % with the SAL antibody, which is consistent with their analogous chemical structures [31]. Therefore, it can be concluded that the immunosensor possesses a satisfactory specificity to salbutamol.…”

Section: Reproducibility Stability and Specificity Of The Immunosensorsupporting

confidence: 62%

CdSe Quantum Dots Based Electrochemiluminescence Immunosensor with Simple Structure for Ultrasensitive Detection of Salbutamol

et al. 2014

Self Cite

View full text Add to dashboard Cite

A rapid and ultrasensitive electrochemiluminescence (ECL) competitive immunoassay based on CdSe quantum dots (QDs) and the shorter chain as possible (cysteamine and glutaraldehyde) has been designed for the detection of salbutamol (SAL). Cysteamine and glutaraldehyde made coating antigen immobilize well on the gold electrode surface through the reaction between functional groups, which brought about the simplicity of the immunosensor to some extent. Transmission electron microscopy image, dynamic light scattering, photoluminescence, ultraviolet‐visible absorption and electrochemical impedance spectra were used to characterize the prepared CdSe QDs and the cysteamine/glutaraldehyde/Ovalbumin‐SAL/anti‐SAL‐QDs immunosensor. In the air‐saturated PBS buffer containing 0.1 M K2S2O8 and 0.1 M KCl (pH 9.0), a strong ECL emission of QDs can be observed which depended linearly on the logarithm of the salbutamol concentration with a wide range from 0.05 ng mL−1 to 100 ng mL−1, and a detection limit of 0.0056 ng mL−1. The sensitivity, repeatability, and specificity of the ECL immunosensor have been evaluated. The sensor has been applied to real samples with satisfactory results. This work will open new ways of detecting food additive residue based on QDs ECL in immunoassays.

show abstract

Section: Reproducibility Stability and Specificity Of The Immunosensorsupporting

confidence: 62%

CdSe Quantum Dots Based Electrochemiluminescence Immunosensor with Simple Structure for Ultrasensitive Detection of Salbutamol

et al. 2014

Self Cite

View full text Add to dashboard Cite

show abstract

“…To demonstrate the advantages of our established mAb‐ELISA over other pAb‐ELISAs or mAb‐ELISAs for PA, the comparison of different ELISAs including our ELISA for the detection of PA is presented in Table . From Table , it can be seen that except one ultra‐sensitive pAb‐ELISA developed in our laboratory, the sensitivity of our mAb‐ELISA is 1.3 to 39 times higher (e.g. IC 50 value is 1.3 to 39 times lower) than those in other ELISAs, demonstrating the high sensitivity of our mAb‐ELISA.…”

Section: Resultsmentioning

confidence: 79%

Development of a highly sensitive and specific monoclonal antibody based enzyme‐linked immunosorbent assay for the detection of a new β‐agonist, phenylethanolamine A, in food samples

et al. 2016

Self Cite

View full text Add to dashboard Cite

show abstract

“…Chemometric analysis is necessary for extraction of the molecular signatures of adulterants present in the sample (Alves et al, 2003;Reid et al, 2004). This adds to the labor, expertise, and time required for analysis and is the most common drawback for this technique (Cao et al, 2013). The combination of gas chromatography and mass spectrometry (GC-MS) potentially reduces the time of analysis, but its application is greatly limited by the high cost of equipment and operation (Reid et al, 2004).…”

Section: Conventional Methods Used To Detect Adulterants In Cereal Fomentioning

confidence: 99%

“…More recently, ELISA has been incorporated into other techniques to enhance the performance of target detection. For example, Cao et al (2013) used liquid chromatography-tandem mass spectrometry (LC-MS/MS) to verify the analytical model they developed to detect phenylethanolamine-A using ELISA. Although ELISA has high, it is limited by prevalent background artifacts that reduce its net-specific signal output.…”

Section: Enzyme-linked Immunosorbent Assay (Elisa)mentioning

confidence: 99%

A Review of Technologies for Detection and Measurement of Adulterants in Cereals and Cereal Products

Ambrose¹,

Cho²

2014

Journal of Biosystems Engineering

View full text Add to dashboard Cite

Purpose:The continued increase in the world population has triggered an increased demand for food. Cereal grains, flour, and their products constitute the staple diet for most of the world's population. This high demand for food, particularly for cereal-based products, has been exploited for commercial gain through adulteration of food materials. We provide a thorough review of the current developments and limitations of modern, nondestructive analytical techniques used for detection of adulterants in cereals and their products and compare them with conventional methods. Results: Adulterated food poses a serious health risks to humans, animals, and the ecosystem in general. Over the last few decades, the adulteration industry has developed fraudulent practices that often outsmart conventional methods of detection and quality control. Therefore, technological advancements to aid in detection and measurement of adulterants in food products and to ensure food quality and safety are critically important to consumers worldwide. Conclusion: There is a continuous demand for development of nondestructive technology to improve the accuracy and efficiency of detection, measurement, and qualification of adulterants in cereals and other food materials.

show abstract

Development of a highly sensitive and specific enzyme-linked immunosorbent assay (ELISA) for the detection of phenylethanolamine A in tissue and feed samples and confirmed by liquid chromatography tandem mass spectrometry (LC–MS/MS)

Cited by 62 publications

References 22 publications

CdSe Quantum Dots Based Electrochemiluminescence Immunosensor with Simple Structure for Ultrasensitive Detection of Salbutamol

CdSe Quantum Dots Based Electrochemiluminescence Immunosensor with Simple Structure for Ultrasensitive Detection of Salbutamol

Development of a highly sensitive and specific monoclonal antibody based enzyme‐linked immunosorbent assay for the detection of a new β‐agonist, phenylethanolamine A, in food samples

A Review of Technologies for Detection and Measurement of Adulterants in Cereals and Cereal Products

Contact Info

Product

Resources

About