Development of a highly sensitive MIP based-QCM nanosensor for selective determination of cholic acid level in body fluids

Abstract

Determination of cholic acid is very important and necessary in body fluids due to its both pharmaceutical and clinical significance. In this study, a quartz crystal microbalance (QCM) nanosensor, which is imprinted cholic acid, has been developed for the assignation of cholic acid. The cholic acid selective memories have been generated on QCM electrode surface by using molecularly imprinted polymer (MIP) based on methacryloylamidohistidine-copper (II) (MAH-Cu(II)) pre-organized monomer. The cholic acid imprinted nanosensor was characterized by atomic force microscopy (AFM) and then analytical performance of the cholic acid imprinted QCM nanosensor was studied. The detection limit was found to be 0.0065μM with linear range of 0.01-1,000 μM. Moreover, the high value of Langmuir constant (b) (7.3*10(5)) obtained by Langmuir graph showed that the cholic acid imprinted nanosensor had quite strong binding sites affinity. At the last step of this procedure, cholic acid levels in body fluids were determined by the prepared imprinted QCM nanosensor.

Staphylococcus aureus exotoxin detection using potentiometric nanobiosensor for microbial electrode approach with the effects of pH and temperature

“…To produce a suitable polymer pattern around the exotoxin and to ensure better distribution, more aprotic solvent was used to dilute the template. [12] Compared with the bulk method, the sedimentation-polymerization method used a higher volume of solvent, thereby creating a nucleus regeneration opportunity, which led to the production of nanoparticles. In this method, 38 mL of acetonitrile solvent was used, which was gently stirred with 11.32 mL of transverse binding agent and ethylene glycol dimethacrylate.…”

“…The remaining polymer pattern was used as potentiometric sensors in the next stage of the experiment. [3,12] A 1:10 ratio of methanol to acetic acid (as an alcohol-acid solution) was used to extract the exotoxin molecules from the polymer pattern. The mechanism of interaction resulted in the elimination of the hydrogen bonds between the amino acids and the MAA monomer units in the polymer.…”

Development of molecularly ımprınted polymer based quartz crystal mıcrobalance nanosensor for the determınatıon of tryptophan

“…Thanks to this event, conjugate structure to the target molecule occurs. The polymers formed as a result of these processes recognised the structure, size and physicochemical properties of the target molecule [25]. MIPs have a unique selectivity for the target molecule and are resistant to mechanical action, heat, acid, base, water and organic solvents [26].…”

“…In our previous works, we used different metal-chelating monomers via metal coordination-chelation interactions with different molecules such as folic acid, cholic acid and caffeic acid and applied in selective sensing detection. In all of these studies we obtained short response time, wide linear range, low determination limit, high selectivity and suitability to real samples [21,25,27]. Herein we report that designing and synthesising a nanosensor based on MIPs in QCM using a metal-chelate monomer, namely, 2-methacryloamidohistidine-Cu (II) [MAH-Cu (II)] to determine the amount of tryptophan in different foods.…”

Development and application of a quartz crystal microbalance sensor based on molecularly imprinted sol-gel polymer for rapid detection of patulin in foods

“…The increased mass caused by the binding reaction results in a decrease of the frequency response [11,12]. Although QCM exhibits high sensitivity, the lack of selectivity limits the application of the QCM sensor [13]. Molecularly imprinted polymer (MIP) mimic the antigen-antibody binding mode by forming cavities that complement a template molecule's shape and size [14].…”