Development of a histopathological index for skeletal muscle analysis in Rattus norvegicus (Rodentia: Muridae)

Zazula, Matheus Felipe; Andrade, Bárbara Zanardini de; Boaro, Carolina De Toni; Kirsch, Christian Bergmann; Reginato, Aline; Peretti, Ana Luiza; Brancalhão, Rose Meire Costa; Bertolini, Gladson Ricardo Flôr; Naliwaiko, Katya; Guimarães, Ana Tereza Bittencourt; Ribeiro, Lucinéia de Fátima Chasko

doi:10.1016/j.acthis.2022.151892

Cited by 5 publications

(5 citation statements)

References 60 publications

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“…This study evaluated the histopathological changes in the tibialis anterior muscle of Wistar rats after remobilization using therapeutic ultrasound for 3 weeks, in order to confirm its effectiveness. The histopathological index presented by Zazula et al (22), was used for this purpose. This approach allows a detailed assessment of changes and lesions in muscle tissue, providing relevant information for understanding the damage that occurred in the context of this study, and an index with a significantly lower value was observed in the ultrasound-treated group, indicating a better restoration of tissue patterns within normality, i.e.…”

Section: Discussionmentioning

confidence: 99%

“…We used 21 male Wistar rats, aged 10 weeks, obtained from the Central Bioterium of the State University of Western Paraná. They were kept in polypropylene boxes, with access to water and food at will, room temperature maintained at 22…”

Section: Methodsmentioning

confidence: 99%

“…With a view to the striated skeletal muscle, evaluations of infiltrates and hypercellularity have been analyzed in the literature (21). The changes caused by both immobilization and remobilization can be assessed quantitatively by a histopathological index proposed by Zazula et al (22) which analyses different degrees of tissue damage, with good replicability and parsimony. In view of the gap in this type of analysis in remobilization aided by the effects of therapeutic ultrasound, the aim of this study was to evaluate the histopathological changes in the tibialis anterior muscle of Wistar rats after remobilization using therapeutic ultrasound.…”

Section: Introductionmentioning

confidence: 99%

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Effect of Therapeutic Ultrasound on the Recovery of the Tibialis Anterior Muscle in Remobilized Wistar Rats after a Period of Immobilization: An Analysis Using the Pathological Index

Kufner Meassi,

de Souza Oliveira,

Pedroso de Oliveira

et al. 2024

Muscle Ligaments and Tendons J

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effect of Therapeutic Ultrasound on the Recovery of the Tibialis Anterior Muscle in Remobilized Wistar Rats after a Period of Immobilization: An Analysis Using the Pathological Index

Kufner Meassi,

de Souza Oliveira,

Pedroso de Oliveira

et al. 2024

Muscle Ligaments and Tendons J

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“…For the characterization of the structural alterations of the muscular tissue, five patterns of reaction (23) were used. Based on the previously described reaction patterns, the slides were evaluated blindly, classifying the reaction patterns and their extensions in the sampling areas.…”

Section: Histopathological Index Analysismentioning

confidence: 99%

Peripubertal Exposure to Glyphosate-Based Herbicides Promotes Histopathological Impairment in the Structure of the Diaphragm Muscle of C57BL/6 Mice

Zazula,

Marca,

de Campos Oliveira

et al. 2024

Muscle Ligaments and Tendons J

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Introduction.Glyphosate is an organophosphate herbicide most used in Latin America, with multisystemic effects, including the respiratory system. In this sense, the objective of this research was to analyze the muscle fibers and neuromuscular junctions (NMJs) of the diaphragm muscle of adult mice exposed to the glyphosate-based herbicide in the peripubertal period. Methods. Twelve male mice were used, divided into a control group (CTL, n = 6), which received water and a glyphosate-based herbicide group (GBH, n = 6), which received 50 mg/Kg/day of Roundup ® , both by gavage from 30 to 60 days. At 150 days, the animals were euthanized, and the diaphragm was collected for analysis of the muscle fibers through hematoxylin-eosin, Masson's trichrome and Picrossirius Red and the NMJs through the nonspecific esterases reaction. Results. Rounded fibers, hypereosinophilic sarcoplasm and enlarged nuclei were found predominantly in GBH. A reduction in body weight, an increase in muscle fiber morphometry, an increase in type III collagen, a decrease in the overlapping of type I and III collagen, and an increase in the area and greater diameter of the NMJs in GBH were observed. Conclusions. Peripubertal exposure to glyphosate-based herbicides showed morphological changes characteristic of muscle degeneration and altered the morphometry of muscle fibers and NMJs of the diaphragm of adult mice.

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“…For the characterization of the structural alterations of the muscular tissue, ve patterns of reaction (Zazula et al 2022) were used. Based on the previously described reaction patterns, the slides were evaluated blindly, classifying the reaction patterns and their extensions in the sampling areas.…”

Section: Histopathological Index Analysismentioning

confidence: 99%

Peripubertal Exposure to Glyphosate-Based Herbicides Promotes Histopathological Impairment in the Structure of the Diaphragm Muscle of c57bl/6 Mice

Zazula

Marca

Oliveira

et al. 2023

Preprint

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Introduction: Glyphosate is an organophosphate herbicide most used in Latin America, with multisystemic effects, including the respiratory system. In this sense, the objective of this research was to analyze the muscle fibers and neuromuscular junctions (JNMs) of the diaphragm muscle of adult mice exposed to the glyphosate-based herbicide in the peripubertal period. Methods: Twelve male mice were used, divided into a control group (CTL, n=6), which received water and a glyphosate-based herbicide group (GBH, n=6), which received 50 mg/Kg/day of Roundup®, both by gavage from 30 to 60 days. At 150 days, the animals were euthanized and the diaphragm was collected for analysis of the muscle fibers through hematoxylin-eosin, Masson's trichrome and Picrossirius Red and the JNMs through the nonspecific esterases reaction. Results: Rounded fibers, hypereosinophilic sarcoplasm and enlarged nuclei were found predominantly in GBH. A reduction in body weight, an increase in muscle fiber morphometry, an increase in type III collagen, a decrease in the overlapping of type I and III collagen, and an increase in the area and greater diameter of the JNMs in GBH were observed. Conclusion: Peripubertal exposure to glyphosate-based herbicides showed morphological changes characteristic of muscle degeneration and altered the morphometry of muscle fibers and JNMs of the diaphragm of adult mice.

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Development of a histopathological index for skeletal muscle analysis in Rattus norvegicus (Rodentia: Muridae)

Cited by 5 publications

References 60 publications

Effect of Therapeutic Ultrasound on the Recovery of the Tibialis Anterior Muscle in Remobilized Wistar Rats after a Period of Immobilization: An Analysis Using the Pathological Index

Effect of Therapeutic Ultrasound on the Recovery of the Tibialis Anterior Muscle in Remobilized Wistar Rats after a Period of Immobilization: An Analysis Using the Pathological Index

Peripubertal Exposure to Glyphosate-Based Herbicides Promotes Histopathological Impairment in the Structure of the Diaphragm Muscle of C57BL/6 Mice

Peripubertal Exposure to Glyphosate-Based Herbicides Promotes Histopathological Impairment in the Structure of the Diaphragm Muscle of c57bl/6 Mice

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