Development of a hollow fiber membrane micro-reactor for biocatalytic production of formate from CO2

Chen

ACS Appl. Mater. Interfaces

et al. 2018

Enzymatic CO reduction can provide value-added chemicals from greenhouse gases at ambient temperature and pressure. However, poor solubility of CO results in a low conversion rate. In this work, polyethylenimine (PEI) was attached onto the surface of poly(acrylic acid)-grafted (PAA-grafted) polyethylene membranes, and then, the membranes were used in an integrated process of CO capture and in situ hydrogenation. Modification conditions were optimized with a surface amino group density of PEI-modified membranes as the characteristic parameter, and then, SEM, FTIR, and XPS analyses were conducted. The effect of PEI-modified membranes on enzyme-catalyzed CO conversion to formic acid, regeneration conditions, and reusability were studied. The results show that when the grafting ratio of PAA increased, surface amino group density of PEI-modified membranes increased up to 6.00 × 10 mol/cm and then kept constant. The optimum modification time, temperature, and PEI concentration were 40 min, 40 °C, and 0.3 wt %. With the same concentration, PEI-1800 could bring more amino groups than PEI-600. SEM, FTIR, and XPS results further confirmed PEI attachment. Introduction of membrane-supported PEI with 5.86 × 10 mol of amino groups facilitated greatly enzymatic CO hydrogenation, and the initial reaction rate increased from 0.280 to 6.90 μM/min. After being regenerated in ammonia, PEI-modified membranes could be reused, and the relative reaction rate was, respectively, 88.0% and 65.7% after 5 and 10 cycles.

Section: Methodsmentioning

confidence: 99%

Polyethylenimine-Modified Membranes for CO₂ Capture and in Situ Hydrogenation

Chen

ACS Appl. Mater. Interfaces

et al. 2018

“…Having a membrane-based system also renders these bioreactors particularly susceptive to fouling—as the capillary membrane pores clog, either due to the cells or other solids, mass transfer through the capillaries becomes increasingly difficult [ 91 ]. The high cell densities which can be obtained in a hollow fibre reactor, along with the resulting high product titres, have led this configuration to be attractive for the production of recombinant proteins, monoclonal antibodies, and viruses [ 92 , 93 , 94 ] while also seeing the use for wastewater treatment [ 95 , 96 ] and biocatalytic reactions [ 97 , 98 ]. Furthermore, hollow fibre bioreactors have already been applied for the bioproduction of stem cells for clinical trials [ 43 ].…”

Section: Single-use Bioreactors For Stem Cell Biomanufacturingmentioning

confidence: 99%

Single-Use Bioreactors for Human Pluripotent and Adult Stem Cells: Towards Regenerative Medicine Applications

Rodrigues

2021

Bioengineering

Research on human stem cells, such as pluripotent stem cells and mesenchymal stromal cells, has shown much promise in their use for regenerative medicine approaches. However, their use in patients requires large-scale expansion systems while maintaining the quality of the cells. Due to their characteristics, bioreactors have been regarded as ideal platforms to harbour stem cell biomanufacturing at a large scale. Specifically, single-use bioreactors have been recommended by regulatory agencies due to reducing the risk of product contamination, and many different systems have already been developed. This review describes single-use bioreactor platforms which have been used for human stem cell expansion and differentiation, along with their comparison with reusable systems in the development of a stem cell bioprocess for clinical applications.

“…Han等 [81] [88] , 如优化固定化酶/辅因子系统, 促进辅酶 NADH再生 [89] ; GelCSi微胶囊分区定位, 根据酶的活性来调节单个酶的量 [90] ; 通过中空纤维膜(HFMs)微反应器, 可将酶活性提高5倍 [91] . [84] ; (b) CO 2 •− 在扫描电子显微镜下的采集示意 [85] (网络版彩图)…”

Section: 度升高聚合离子液体动力学半径减小聚合离子液体unclassified

CO<sub>2</sub> chemical engineering: CO<sub>2</sub> green conversion enhanced by ionic liquid microhabitat

et al. 2019