Development of a Homologous Fluorescence Polarization Immunoassay for Diisobutyl Phthalate in Romaine Lettuce

Chen, Yingshan; Cui, Xiping; Wu, Panpan; Jiang, Zhengyun; Jiao, Luoying; Hu, Qingqing; Eremin, Sergei A.; Zhao, Suqing

doi:10.1007/s12161-016-0596-1

Cited by 12 publications

(5 citation statements)

References 34 publications

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“…In one case it is branched, in the other, it is linear with an equal number of carbon atoms. A comparison with the works of predecessors [14,17,18], which obtained antibodies to DiBP for fluorescent polarization immunoassay, showed the following results when compared with six phthalic acid esters. Chen et al [18] observed the cross-reactivities of 7.37, 1.62, 1.53, and 1.07% for DCHP, BBP, DMP, DBP, DEP, and DnOP, respectively.…”

Section: Characterization Of Antisera By Colorimetric Elisamentioning

confidence: 87%

“…antibodies to DiBP for fluorescent polarization immunoassay, showed the following results when compared with six phthalic acid esters. Chen et al [18] observed the crossreactivities of 7.37, 1.62, 1.53, and 1.07% for DCHP, BBP, DMP, DBP, DEP, and DnOP, respectively. Authors suggested that increasing cross-reactivity is related to a decrease in antibody recognition ability when the side radical takes a cyclic form and creates steric hindrance.…”

Section: The Use Of Methanol In the Working Buffer During Competitive...mentioning

confidence: 99%

“…after pretreatment [16]. The previously described developments for the determination of DiBP by immunoassay techniques are limited by the study on polarization fluorescent immunoassays (FPIAs) [14,17,18].…”

Section: Introductionmentioning

confidence: 99%

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Development of Chemiluminescent ELISA for Detection of Diisobutyl Phthalate in Water, Lettuce and Aquatic Organisms

et al. 2023

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The use of plasticizers to improve the quality of plastics widely used for household purposes inevitably leads to an increase in their pollution of food and environmental objects. Diisobutyl phthalate (DiBP) is one of the ortho-substituted phthalic acid esters that negatively affect human health and ecosystems. This work is directed to the development of a chemiluminescent enzyme immunoassay (CL-ELISA) for the determination of diisobutyl phthalate in water and food. Luminol, which is oxidized with hydrogen peroxide in the presence of p-iodophenol as an enhancer, was chosen as the substrate for horseradish peroxidase used as a label in the analysis. For this development, rabbit anti-DiBP polyclonal antibodies were generated and tested with the synthesized hapten–protein conjugate. The developed chemiluminescent ELISA has a detection limit of 1.8 ng/mL; the operating range was 5.0–170.8 ng/mL at a content of 10% methanol in the assay medium. The assay was successfully applied to detect diisobutyl phthalate in lettuce leaves, seafood, and water. When using extraction with methanol and hexane, the recovery of DiBP in samples varies in the range of 76.9–134.2%; for assays in natural waters, the recovery rates are from 79.5 to 113.4%.

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Section: Characterization Of Antisera By Colorimetric Elisamentioning

confidence: 87%

Section: The Use Of Methanol In the Working Buffer During Competitive...mentioning

confidence: 99%

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Development of Chemiluminescent ELISA for Detection of Diisobutyl Phthalate in Water, Lettuce and Aquatic Organisms

et al. 2023

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“…We added 500 µL antibody working solution into the tube, then 500 µL OTA-EDF (or OTA-BDF/OTA-AMF/OTA-HDF) working solution and mixed. The FP value was measured after 10 min of oscillate incubation at ambient temperature; the relative FP mean values (mP/mP 0 ) were used in the inhibition curve to standardize the FP value, where mP is the current FP value of different OTA concentrations and mP 0 is the value of blank-control (50 µL methanol-BB solution was used as the blank-control) [41]. The values of mP/mP 0 were plotted against OTA concentration [37].…”

Section: Methods Of Fluorescence Polarization Immunoassaymentioning

confidence: 99%

Development of an Ultrasensitive and Rapid Fluorescence Polarization Immunoassay for Ochratoxin A in Rice

Huang

Tang

Jallow

et al. 2020

Toxins

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Ochratoxin A (OTA) is a known food contaminant that affects a wide range of food and agricultural products. The presence of this fungal metabolite in foods poses a threat to human health. Therefore, various detection and quantification methods have been developed to determine its presence in foods. Herein, we describe a rapid and ultrasensitive tracer-based fluorescence polarization immunoassay (FPIA) for the detection of OTA in rice samples. Four fluorescent tracers OTA-fluorescein thiocarbamoyl ethylenediamine (EDF), OTA-fluorescein thiocarbamoyl butane diamine (BDF), OTA-amino-methyl fluorescein (AMF), and OTA-fluorescein thiocarbamoyl hexame (HDF) with fluorescence polarization values (δFP = FPbind-FPfree) of 5, 100, 207, and 80 mP, respectively, were synthesized. The tracer with the highest δFP value (OTA-AMF) was selected and further optimized for the development of an ultrasensitive FPIA with a detection range of 0.03–0.78 ng/mL. A mean recovery of 70.0% to 110.0% was obtained from spiked rice samples with a relative standard deviation of equal to or less than 20%. Good correlations (r2 = 0.9966) were observed between OTA levels in contaminated rice samples obtained by the FPIA method and high-performance liquid chromatography (HPLC) as a reference method. The rapidity of the method was confirmed by analyzing ten rice samples that were analyzed within 25 min, on average. The sensitivity, accuracy, and rapidity of the method show that it is suitable for screening and quantification of OTA in food samples without the cumbersome pre-analytical steps required in other mycotoxin detection methods.

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“…With the heterologous tracer 5-OH-DCF-AMF, approximately the same sensitivity as with the homologous tracer DCF-Ahx-AMF was obtained (compare Table 2). According to some studies [27][28][29], heterologous tracers may improve the sensitivity.…”

Section: Sensitivity and Measurement Rangementioning

confidence: 99%

Fluorescence polarization immunoassay for the determination of diclofenac in wastewater

et al. 2020

Self Cite

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Pharmacologically active compounds are often detected in wastewater and surface waters. The nonsteroidal anti-inflammatory drug diclofenac (DCF) was included in the European watch list of substances that requires its environmental monitoring in the member states. DCF may harmfully influence the ecosystem already at concentrations ≤ 1 μg L−1. The fast and easy quantification of DCF is becoming a subject of global importance. Fluorescence polarization immunoassay (FPIA) is a homogeneous mix-and-read method which does not require the immobilization of reagents. FPIA can be performed in one phase within 20–30 min, making it possible to analyse wastewater without any complicated pre-treatment. In this study, new tracer molecules with different structures, linking fluorophores to derivatives of the analyte, were synthesized, three homologous tracers based on DCF, two including a C6 spacer, and one heterologous tracer derived from 5-hydroxy-DCF. The tracer molecules were thoroughly assessed for performance. Regarding sensitivity of the FPIA, the lowest limit of detection reached was 2.0 μg L−1 with a working range up to 870 μg L−1. The method was validated for real wastewater samples against LC-MS/MS as reference method with good agreement of both methods.

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Development of a Homologous Fluorescence Polarization Immunoassay for Diisobutyl Phthalate in Romaine Lettuce

Cited by 12 publications

References 34 publications

Development of Chemiluminescent ELISA for Detection of Diisobutyl Phthalate in Water, Lettuce and Aquatic Organisms

Development of Chemiluminescent ELISA for Detection of Diisobutyl Phthalate in Water, Lettuce and Aquatic Organisms

Development of an Ultrasensitive and Rapid Fluorescence Polarization Immunoassay for Ochratoxin A in Rice

Fluorescence polarization immunoassay for the determination of diclofenac in wastewater

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