Development of a HPLC/Tandem-MS Method for the Analysis of the Larvicides Methoprene, Hydroprene, and Kinoprene at Trace Levels Using Diels−Alder Derivatization

Aronov, Pavel A.; Dettmer, Katja; Christiansen, J. A.; Cornel, and Anthony J.; Hammock, Bruce D.

doi:10.1021/jf0485842

Cited by 9 publications

(4 citation statements)

References 8 publications

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“…These larvicides were used to prevent the invasion and subsequent spread of the mosquito‐borne West Nile virus in the USA. The derivatization improved the limit of detection 100‐fold and the developed method was applied to measure the methoprene concentrations in water from a treated site (Aronov, Dettmer, Christiansen, Cornel, & Hammock, ). PTAD was also applicable to the analysis of ergosterol (D 2 precursor) and exemestane (a drug used to treat breast cancer; Higashi, unpublished data).…”

Section: Application Of Cookson‐type Reagents To Analysis Of Compoundmentioning

confidence: 99%

Application of Cookson‐type reagents for biomedical HPLC and LC/MS analyses: a brief overview

Higashi

Shimada

2016

Biomedical Chromatography

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High-performance liquid chromatography (HPLC), LC/mass spectrometry (MS) and LC/tandem mass spectrometry (MS/MS) have been widely used for biomedical analyses, in which chemical derivatization is one of the most important methods to increase the sensitivity and selectivity. A Cookson-type reagent [4-substituted-1,2,4-triazoline-3,5-dione (4-substituted-TAD)] reacts with the compound bearing a conjugated diene, such as the vitamin D compound, to quantitatively form the stable Diels-Alder adduct. The reagent with a chromophore or fluorophore at the 4-position of TAD yields a highly responsive adduct for the UV or fluorescence detection, respectively. The Diels-Alder adduct with a Cookson-type reagent having a permanently charged, proton-affinitive or electron-affinitive moiety is sensitively detected by a specific MS analyzer. This paper is a brief overview of the applications of the reagents for biomedical analyses mainly using HPLC or LC/MS(/MS).

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Section: Application Of Cookson‐type Reagents To Analysis Of Compoundmentioning

confidence: 99%

Application of Cookson‐type reagents for biomedical HPLC and LC/MS analyses: a brief overview

Higashi

Shimada

2016

Biomedical Chromatography

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“…This was followed by chromatographic separation using ultra performance liquid chromatography (UPLC) with detection by tandem quatropole mass spectrometry using positive electrospray ionization. 17…”

Section: Mass Spectroscopymentioning

confidence: 99%

Vitamin D Enhances Corneal Epithelial Barrier Function

Yin

Pintea

Lin

et al. 2011

Invest. Ophthalmol. Vis. Sci.

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107

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“…However, the conjugated diene group of vitamin D metabolites makes them a specific target for Diels-Alder derivatization. In fact, several highly reactive 4-substituted 1,2,4-triazoline-3,5-diones (TADs or Cooksontype reagents) have been reported in the literature for the analysis of vitamin D metabolites, their analogs and other dienes, including derivatization reagents for ESI-MS [6,[17][18][19][20][21][22]. The derivatization reagents introduce polar groups and thus typically result in a 100-1000-fold increase in sensitivity over nonderivatized compounds.…”

Section: Introductionmentioning

confidence: 99%

Metabolic profiling of major vitamin D metabolites using Diels–Alder derivatization and ultra-performance liquid chromatography–tandem mass spectrometry

et al. 2008

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Biologically active forms of vitamin D are important analytical targets in both research and clinical practice. The current technology is such that each of the vitamin D metabolites is usually analyzed by individual assay. However, current LC-MS technologies allow the simultaneous metabolic profiling of entire biochemical pathways. The impediment to the metabolic profiling of vitamin D metabolites is the low level of 1alpha,25-dihydroxyvitamin D(3) in human serum (15-60 pg/mL). Here, we demonstrate that liquid-liquid or solid-phase extraction of vitamin D metabolites in combination with Diels-Alder derivatization with the commercially available reagent 4-phenyl-1,2,4-triazoline-3,5-dione (PTAD) followed by ultra-performance liquid chromatography (UPLC)-electrospray/tandem mass spectrometry analysis provides rapid and simultaneous quantification of 1alpha,25-dihydroxyvitamin D(3), 1alpha,25-dihydroxyvitamin D(2), 24R,25-dihydroxyvitamin D(3), 25-hydroxyvitamin D(3) and 25-hydroxyvitamin D(2) in 0.5 mL human serum at a lower limit of quantification of 25 pg/mL. Precision ranged from 1.6-4.8 % and 5-16 % for 25-hydroxyvitamin D(3) and 1alpha,25-dihydroxyvitamin D(3), respectively, using solid-phase extraction.

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Development of a HPLC/Tandem-MS Method for the Analysis of the Larvicides Methoprene, Hydroprene, and Kinoprene at Trace Levels Using Diels−Alder Derivatization

Cited by 9 publications

References 8 publications

Application of Cookson‐type reagents for biomedical HPLC and LC/MS analyses: a brief overview

Application of Cookson‐type reagents for biomedical HPLC and LC/MS analyses: a brief overview

Vitamin D Enhances Corneal Epithelial Barrier Function

Metabolic profiling of major vitamin D metabolites using Diels–Alder derivatization and ultra-performance liquid chromatography–tandem mass spectrometry

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