Development of a HPLC-UV method for determination of meloxicam in human plasma and pharmaceutical dosage forms

Abstract

Objectives: A simple, quick and low cost HPLC-UV method for assay of meloxicam in plasma and pharmaceutical dosage forms was developed. Methods: Separation and assay of meloxicam, using a simple reverse phase HPLC-UV method was achieved using an Agilent Zorbax SB C18 column, with methanol and 1% aqueous solution of glacial acetic acid as mobile phase. Elution was performed with composition gradient, meloxicam being detected at 355 nm with a 5 minutes analysis time. The method was tested on human plasma and pharmaceutical dosage forms. Results: The retention time of the meloxicam was 3,7 minutes. Regression analysis showed good linearity, with correlation coefficient R= 0,9997; linear regression equation: y = 206,1x -77,5 over the 20-2000 ng/ml concentration range. Limit of detection was determined to be 5 ng/ml and limit of quantification was set at 15 ng/ml. The recovery of the analyte in human plasma was low: 30,50%, however it was reproducible, with a coefficient of variation of 4,83%. The analysis of the tablets resulted in a 85,82% of meloxicam compared to the declared concentration. Conclusions: The method proposed is quick, simple and adequate for detecting the meloxicam in human plasma. Although the recovery rate was low, it was reproducible, which leads to the fact, that improving extraction procedure can optimize the method.

The effect of surgery (Ovariohysterectomy) on the plasma disposition of meloxicam following intravenous administration in dogs

“…The parent compounds of MLX in dog plasma were analysed using validated high performance liquid chromatography (HPLC) following a liquid-liquid phase extraction procedure. Plasma concentrations of MLX were measured by minor modifications of the methods described by Eniko et al [ 10 ].…”

Development and Validation of Analytical Method for Simultaneous Estimation of Bupivacaine and Meloxicam in Human Plasma Using UPLC-MS/MS

“…shorter runtime, less sample volume and can estimate both compounds in a single run. In the current method Bupivacaine-d9 (BPd 9 ) and 16 c, d 3 Meloxicam (ME 13 cd 3 ) were used as internal standards, the chemical structure of BP is shown in Figure 1. This method can be applied to pharmacokinetic study of plasma BP and ME concentration after oral administration.…”

Effect of Andrographolide Co-Administration on Pharmacokinetics of Meloxicam in Rats

“…Meloxicam concentration in plasma samples was determined by reverse-phase High Performance Liquid Chromatography (HPLC) after extraction, using reported assays (Eniko et al, 2014;Gondaliya et al, 2015;Patel et al, 2016) with minor modifications. The high performance liquid chromatography apparatus of Shimadzu comprising binary gradient delivery pump (model LC -20AP) and UV detector (model CBM-20A) was used for assay.…”