Development of a human corneal epithelium model utilizing a collagen vitrigel membrane and the changes of its barrier function induced by exposing eye irritant chemicals

Takezawa, Toshiaki; Nishikawa, Kazunori; Wang, Pichao

doi:10.1016/j.tiv.2011.05.021

Cited by 31 publications

(22 citation statements)

References 14 publications

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“…It exhibits excellent transparency and permeability for high‐molecular weight proteins and has, therefore, been successfully used as a cell culture scaffold in various studies. Human corneal models (Ko et al, ; McIntosh Ambrose et al, ; Takezawa, Nishikawa, & Wang, ; Chae et al, ; Yamaguchi et al, 2016; Yoshida et al, ; Yamaguchi & Takezawa, ) and human hepatocytes models (Oshikata‐Miyazaki & Takezawa, ; Watari et al, ) have been successfully reconstructed using the collagen vitrigel membrane. An artificial skin based on collagen vitrigel was shown to promote the epithelialization of regenerative skin and to inhibit scar formation and inflammation, whereas the existing products such as DuoACTIVEV ET of a hydrocolloid dressing and Pelnac of a collagen sponge was not able to sufficiently inhibit scar formation (Aoki et al, ).…”

Section: Discussionmentioning

confidence: 99%

Transplantation of multiciliated airway cells derived from human iPS cells using an artificial tracheal patch into rat trachea

Okuyama

Ohnishi

Nakamura

et al. 2019

J Tissue Eng Regen Med

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Tracheal resection is often performed for malignant tumours, congenital anomalies, inflammatory lesions, and traumatic injuries. There is no consensus on the best approach for the restoration of tracheal functionality in patients with tracheal defects. Artificial grafts made of polypropylene and collagen sponge have been clinically used by our group. However, 2 months are required to achieve adequate epithelialization of the grafts in humans. This study aimed to investigate the feasibility of transplantation therapy using an artificial trachea with human‐induced pluripotent stem cell (hiPSC)‐derived multiciliated airway cells (hiPSC‐MCACs). Collagen vitrigel membrane, a biocompatible and absorbable material, was used as a scaffold to cover the artificial trachea with hiPSC‐MCACs. Analyses of hiPSC‐MCACs on collagen vitrigel membrane were performed by immunocytochemistry and electron microscopy and by assessing ciliary beat frequency. Along with the artificial trachea, hiPSC‐MCACs were transplanted into surgically created tracheal defects of immunodeficient rats. The survival of transplanted cells was histologically evaluated at 1 and 2 weeks after the transplantation. The hiPSC‐MCACs exhibited motile cilia on collagen vitrigel membrane. The surviving hiPSC‐MCACs were observed in the endotracheal epithelium of the tracheal defect at 1 and 2 weeks after transplantation. These results suggest that hiPSC‐MCAC is a useful candidate for tracheal reconstruction.

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Section: Discussionmentioning

confidence: 99%

Transplantation of multiciliated airway cells derived from human iPS cells using an artificial tracheal patch into rat trachea

Okuyama

Ohnishi

Nakamura

et al. 2019

J Tissue Eng Regen Med

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“…We established a preparation method of a corneal epithelium model utilizing an air–liquid interface culture system that facilitates induction of layering rabbit corneal epithelial cells cultured on the CVM scaffold (Takezawa et al ., 2008). To overcome species differences between human and rabbit in sensitivity to exogenous chemicals, we developed a human corneal epithelium (HCE) model by three‐dimensionally culturing HCE‐T cells on the CVM scaffold (Takezawa et al ., 2011a). Here, the scaffold was fabricated on a polyethylene terephthalate (PET) membrane of a Millicell chamber appropriate for the transepithelial electrical resistance (TEER) assay of epithelial cells.…”

Section: Introductionmentioning

confidence: 99%

“…TEER is known as a suitable method for evaluating the integrity of the tight junction of corneal epithelium in vivo (Uematsu et al ., 2007). We examined four chemicals using the HCE model, and consequently demonstrated that the time‐dependent relative changes of TEER are useful indicators to assess ocular irritancy effects of chemicals even in the middle category (Takezawa et al ., 2011a). However, this model is inappropriate for immunohistological analyses due to difficulty in preparing its frozen sections, including the PET membrane.…”

Section: Introductionmentioning

confidence: 99%

Predictive performance of the Vitrigel‐eye irritancy test method using 118 chemicals

Yamaguchi

Kojima²,

Takezawa

2015

J of Applied Toxicology

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We recently developed a novel Vitrigel‐eye irritancy test (EIT) method. The Vitrigel‐EIT method is composed of two parts, i.e., the construction of a human corneal epithelium (HCE) model in a collagen vitrigel membrane chamber and the prediction of eye irritancy by analyzing the time‐dependent profile of transepithelial electrical resistance values for 3 min after exposing a chemical to the HCE model. In this study, we estimated the predictive performance of Vitrigel‐EIT method by testing a total of 118 chemicals. The category determined by the Vitrigel‐EIT method in comparison to the globally harmonized system classification revealed that the sensitivity, specificity and accuracy were 90.1%, 65.9% and 80.5%, respectively. Here, five of seven false‐negative chemicals were acidic chemicals inducing the irregular rising of transepithelial electrical resistance values. In case of eliminating the test chemical solutions showing pH 5 or lower, the sensitivity, specificity and accuracy were improved to 96.8%, 67.4% and 84.4%, respectively. Meanwhile, nine of 16 false‐positive chemicals were classified irritant by the US Environmental Protection Agency. In addition, the disappearance of ZO‐1, a tight junction‐associated protein and MUC1, a cell membrane‐spanning mucin was immunohistologically confirmed in the HCE models after exposing not only eye irritant chemicals but also false‐positive chemicals, suggesting that such false‐positive chemicals have an eye irritant potential. These data demonstrated that the Vitrigel‐EIT method could provide excellent predictive performance to judge the widespread eye irritancy, including very mild irritant chemicals. We hope that the Vitrigel‐EIT method contributes to the development of safe commodity chemicals. Copyright © 2015 The Authors. Journal of Applied Toxicology published by John Wiley & Sons Ltd.

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“…81,82 The total reconstruction of the cornea in rabbits was carried out by impregnation of supports of fibrin agarose with corneal epithelial, stromal, and stem cells, precultured in vitro. 83 The collagen hydrogel glazing technique, developed by Takezawa et al, 84 resulted in a membrane having optical transparency, and adequate mechanical resistance, similar to that of connective tissue from Bowman's membrane.…”

Section: Scaffolds: Physical and Substrate Porous Supports For Seedinmentioning

confidence: 99%

Scaffolds and tissue regeneration: An overview of the functional properties of selected organic tissues

et al. 2015

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Tissue engineering plays a significant role both in the re-establishment of functions and regeneration of organic tissues. Success in manufacturing projects for biological scaffolds, for the purpose of tissue regeneration, is conditioned by the selection of parameters such as the biomaterial, the device architecture, and the specificities of the cells making up the organic tissue to create, in vivo, a microenvironment that preserves and further enhances the proliferation of a specific cell phenotype. To support this approach, we have screened scientific publications that show biomedical applications of scaffolds, biomechanical, morphological, biochemical, and hemodynamic characteristics of the target organic tissues, and the possible interactions between different cell matrices and biological scaffolds. This review article provides an overview on the biomedical application of scaffolds and on the characteristics of the (bio)materials commonly used for manufacturing these biological devices used in tissue engineering, taking into consideration the cellular specificity of the target tissue. © 2015 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 104B: 1483-1494, 2016.

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Development of a human corneal epithelium model utilizing a collagen vitrigel membrane and the changes of its barrier function induced by exposing eye irritant chemicals

Cited by 31 publications

References 14 publications

Transplantation of multiciliated airway cells derived from human iPS cells using an artificial tracheal patch into rat trachea

Transplantation of multiciliated airway cells derived from human iPS cells using an artificial tracheal patch into rat trachea

Predictive performance of the Vitrigel‐eye irritancy test method using 118 chemicals

Scaffolds and tissue regeneration: An overview of the functional properties of selected organic tissues

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