Development of a Hybrid Biomimetic Enamel-Biocomposite Interface and a Study of Its Molecular Features Using Synchrotron Submicron ATR-FTIR Microspectroscopy and Multivariate Analysis Techniques

Abstract

Using a biomimetic strategy and bioinspired materials, our work proposed a new technological approach to create a hybrid transitional layer between enamel and dental biocomposite. For this purpose, an amino acid booster conditioner based on a set of polar amino acids (lysine, arginine, hyaluronic acid), calcium alkali, and a modified adhesive based on BisGMA and nanocrystalline carbonate-substituted hydroxyapatite are used during dental enamel restoration. The molecular properties of the hybrid interface formed using the proposed strategy were understood using methods of multivariate statistical analysis of spectral information collected using the technique of synchrotron infrared microspectroscopy. The results obtained indicate the possibility of forming a bonding that mimics the properties of natural tissue with controlled molecular properties in the hybrid layer. The diffusion of the amino acid booster conditioner component, the calcium alkali, and the modified adhesive with nanocrystalline carbonate-substituted hydroxyapatite in the hybrid interface region creates a structure that should stabilize the reconstituted crystalline enamel layer. The developed technology can form the basis for an individualized, personalized approach to dental enamel restorations.

Synchrotron X-ray Studies of the Structural and Functional Hierarchies in Mineralised Human Dental Enamel: A State-of-the-Art Review

“…Molecular analysis using FTIR and attenuated total reflection (ATR)-FTIR is suitable for studying the interface of different materials, as well as to evaluate their interactions. This approach was utilised to study biomimetic composites, dental materials, dentine and enamel interface, and phosphate and amide groups (corresponding to mineral and organic components respectively [ 324 , 325 , 326 , 337 , 347 , 352 , 562 ], Figure 9 m), to develop hybrid interfaces. In another study, the distribution of organic and carbonate contents was determined from ancient and modern teeth [ 341 ].…”

A Study of the Association between Primary Oral Pathologies (Dental Caries and Periodontal Diseases) Using Synchrotron Molecular FTIR Spectroscopy in View of the Patient’s Personalized Clinical Picture (Demographics and Anamnesis)

“…The combination of synchrotron radiation and FTIR spectroscopy methods allows one to achieve the best spectral and lateral resolution exceeding (by an order of magnitude) the level of laboratory FTIR instruments, which is extremely important for the analysis of samples collected in macro quantities [ 42 , 43 , 44 , 45 , 46 ].…”

A Study of the Effects of Medical Dental Laser and Diamond Drill on Dentin Tissue during Dental Restoration Based on Spectral Imaging and Multivariate Analysis of Synchrotron FTIR Microspectroscopy Data

“…Using the characteristic signatures of molecular spectroscopy (position, width and shape of the spectral line) chemical/molecular images of the micro/nano region of the sample being diagnosed can be collected and its nature established [8,9]. Subsequent application of multivariate analysis techniques to the large spectral dataset collected allows for the extraction of principle information useful for accurate and rapid monitoring of molecular specific distribution in biotissues, or, for example, the bacterial status in the analyzed area [10][11][12].…”