Development of a lateral flow test to detect metabolic resistance in Bemisia tabaci mediated by CYP6CM1, a cytochrome P450 with broad spectrum catalytic efficiency

Rapid selection of a pyrethroid metabolic enzyme CYP9K1 by operational malaria control activities

“…S1 B ). CYP9K1 likewise metabolized pyriproxyfen, as observed by the NADPH-dependent depletion of this insecticide ( Table 2 ) and the formation of two metabolites, which eluted at 6.2 min and 7.4 min, respectively ( 24 ). In contrast, bendiocarb was not metabolized by CYP9K1, with no substrate depletion after a 20-min incubation, after which spontaneous, non–P450-specific, degradation occurred.…”

Insecticide resistance in Trialeurodes vaporariorum populations and novel diagnostics for kdr mutations

“…The neonicotinoids imidacloprid (98.6% technical, Bayer Crop-Science), clothianidin, dinotefuran, thiamethoxam, nitenpyram, thiacloprid, acetamiprid (99.4% Fluka, pestanal, analytical standard) and pymetrozine (98.4% Fluka, pestanal, analytical standard) were used for the metabolism assays and the incubation of the TvCYP6CM2 and TvCYP6CM3 were performed as described previously. 41,42 Briefly, 25 μM final concentration of insecticides was incubated with bacterial membranes containing 25 pmol recombinant P450 and 75 pmol b5, in 100 μl Tris-HCl buffer (0.2 M, pH 7.4) containing 0.25 mM MgCl 2. The incubation was performed in the presence or absence of NADPH generating system: 1 mM glucose-6-phosphate (Sigma-Aldrich, St. Louis, MO, USA), 0.1 mM NADP + (Sigma-Aldrich), 1 unit ml -1 glucose-6-phosphate dehydrogenase (G6PDH, Sigma-Aldrich).…”

Neonicotinoid resistance in adults and nymphs of Bemisia tabaci (Genn., 1889) (Hemiptera: Aleyrodidae) populations in tomato fields from Tokat, Turkey

“…These are target site resistance and metabolic resistance. It has been determined that especially monooxygenase activity (P450) is caused by neonicotinoid resistance (Karunker et al, 2008;Roditakis et al, 2011;Nauen et al, 2015;Bass et al, 2015;Satar et al, 2018). Continuous use of neonicotinoids has led to resistance in white flies.…”